Total GLP-1 ELISA Kit
产品报价:询价
更新时间:2018/7/26 9:03:19
产地:浙江
品牌:EDI
型号:96t
厂商性质: 生产型,
公司名称: 嘉兴行键生物科技有限公司
Farnk ou : (13967333273) (0573-82809169)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2018/7/26 9:03:19
产地:浙江
品牌:EDI
型号:96t
厂商性质: 生产型,
公司名称: 嘉兴行键生物科技有限公司
Farnk ou : (13967333273) (0573-82809169)
(联系我时,请说明是在来宝网上看到的,谢谢!)
Total GLP-1 ELISA Kit
Catalog Number: KT-876
This ELISA (enzyme-linked immunosorbent assay) kit is produced for the quantitative determination of the total value of glucagon-like peptide-1 (7-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. This kit is for research purpose only.
This ELISA is designed, developed and produced for the quantitative measurement of GLP-1 (7-36) and (9-36) in plasma sample. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 antibodies.
Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidate (HRP) conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36)/(9-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.
REAGENTS: Preparation
1. Streptavidin Coated Microplate:96 wells
2. Total GLP-1 Tracer Antibody:1vial× 0.6 mL
3. Total GLP-1 Capture Antibody:1vial× 0.6 mL
4. ELISA Wash Concentrate,30X:1 bottle× 20mL
5. ELISA HRP Substrate :1 bottle× 24mL
6. ELISA Stop Solution:1 bottle× 12mL
7. Total GLP-1 Standards:5 vials
8. GLP-1 Controls:2 vials
9. Tracer Antibody Diluent:1vials× 12mL
Short Assay Protocol:
- Add 100 μl/well of standards, control and patient sample
- Add 100 μl of Antibody Mixture
- Incubate 20 - 24 hour at 2-8°C, static
- Wash strips with diluted wash buffer
- Add 200 μl/well of TMB substrate
- Incubate 20 min at RT, static
- Add 50 μl stop solution
- Read strips at OD 450 nm/620 nm or 450 nm/650 nm
Frank Ou
Regional Sales Manager
Epitope Biotechnology Co.,Ltd.
599 Yazhong Rd.1-4F,jiaxing,zhejiang
PM: 13967333273
Tel: 0573-82809169
Fax: 0573-82809206
Email: zou@xjbiotech.com
http://www.xjbiotech.com
http://www.epitopediagnostics.com