产品报价：询价
更新时间：2012/11/9 12:07:44
产地：北京
品牌：北京正旦国际科技有限责任公司
型号：ZDK-018L
厂商性质： 生产型,服务型,政府或其他机构,
公司名称： 北京正旦国际科技有限责任公司-蛋白质药物国家工程研究中心
李先生 : () (010-80705573)
（联系我时，请说明是在来宝网上看到的，谢谢！）
Mouse Monoclonal Antibody to HA-tag
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General Information |
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Immunogen: |
HA peptide conjugated with KLH |
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Clone ID: |
Mouse mAb |
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Ig Type: |
IgG1 |
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Applications: |
WB , IHC , IP |
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Specificity: |
Specifically detected HA tag fused protein. |
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Formulation: |
0.2µm filtered solution in PBS with 50％ glycerol |
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Storage: |
<-20℃ |
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with peptide YPYDVPDYA conjugated with KLH. The ascites was purified by ion exchange chromatography.
Applications
1) Western blot (1:1000) : This antibody can be used at 2μg with appropriate secondary reagents to detect HA-tagged proteins.
M 1 2 3 4 Lane 1, 2: 293T control Lane 3, 4: 293T transfected with HA plasmid M: protein ladder kDa
Immunoprecipitation (1:500)：This antibody can be used at 2μg to precipitate HA-tagged proteins.
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Lane 1: HA mAb IP with 293T transfected with HA plasmid Lane2: HA mAb IP with 293T transfected without HA plasmid M: protein ladder |
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M 1 2 |
Immunofluorescence (1:100): This antibody can be used at 2μg to detect HA-tagged proteins.
293T transfected with HA plasmid 1:100 dilution 293T control，1:100 dilution
Specificity
Mouse mAb specifically detects over-expressed proteins containing the HA epitope tag.
Storage
Store at -20℃, and stable for one year from the date of shipment.
