人TNF alpha ELISA试剂盒(ab181421)

Key features and details

One-wash 90 minute protocol

Sensitivity: 4.32 pg/ml

Range: 15.63 pg/ml - 1000 pg/ml

Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Pl

人TNF alpha ELISA试剂盒(ab181421)

• 存放说明

  Store at +4°C. Please refer to protocols.

• 组件	1 x 96 tests	10 x 96 tests	1 x 384 tests
  10X Wash Buffer PT (ab206977)	1 x 20ml	1 x 200ml	1 x 20ml
  384 well CaptSure? microplates	0 x 0 unit	0 x 0 unit	1 unit
  Antibody Diluent 4BR	1 x 6ml	10 x 6ml	1 x 6ml
  Human TNFa Capture Antibody	1 x 600μl	10 x 600μl	1 x 600μl
  Human TNFa Detector Antibody	1 x 600μl	10 x 600μl	1 x 600μl
  Human TNFa Lyophilized Recombinant Protein	2 vials	2 x 10 vials	2 vials
  Plate Seal	1 unit	10 units	1 unit
  Sample Diluent NS (ab193972)	1 x 50ml	2 x 250ml	2 x 50ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit	10 units	0 x 0 unit
  Stop Solution	1 x 12ml	1 x 120ml	2 x 12ml
  TMB Development Solution	1 x 12ml	1 x 120ml	2 x 12ml

• 人TNF alpha ELISA试剂盒(ab181421)

  
  

• 检测方法

  Colorimetric

• 精确度

  批次内

  样品	n	Mean	SD	CV%
  PBMC media	8			2.5%

  批次间

  样品	n	Mean	SD	CV%
  PBMC media	3			3.1%

• 样品类型

  Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid

• 检测类型

  Sandwich (quantitative)

• 灵敏度

  4.32 pg/ml

• 范围

  15.63 pg/ml - 1000 pg/ml

• 回收率

  
  

  特定样本回收率

  样品类型	平均%	范围
  Cell culture supernatant	110	105% - 113%
  Serum	88	83% - 93%
  Hep Plasma	98	89% - 110%
  EDTA Plasma	100	95% - 104%
  Cit plasma	91	86% - 97%
  Cerebral Spinal Fluid	83	80% - 88%

•  人TNF alpha ELISA试剂盒(ab181421)

• 检测时间

  1h 30m

• 实验步骤

  One step assay

• 种属反应性

  与反应: Human
  

• 产品概述

  Human TNF alpha ELISA kit (ab181421) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF alpha protein in human serum, plasma and culture media. It uses our proprietary SimpleStep ELISA? technology. Quantitate human TNFa with 4.32 pg/mL sensitivity.
  
  SimpleStep ELISA? technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA? plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich compl in a single step, significantly reducing assay time. See the SimpleStep ELISA? protocol summary in the image section for further details. Our SimpleStep ELISA? technology provides several benefits:
  
          -Single-wash protocol reduces assay time to 90 minutes or less
          -High sensitivity, specificity and reproducibility from superior antibodies
          -Fully validated in biological samples
          -96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA? microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA? kits.

  
  

  ASSAY SPECIFICITY This kit recognizes both native and recombinant human TNF alpha protein in serum, plasma, and cell culture supernatant samples only.

  
  

  CROSS REACTIVITY Recombinant proteins were prepared at 1000 pg/mL and assayed for cross reactivity. No cross reactivity was found for the following targets: - Human IL-2 - Human IL-4 - Human IL-1 alpha - Human IFN gamma - Human TNF beta - Human TNF R1.
  
  INTERFERENCE Recombinant Human TNF R1 was prepared at 1000 pg/mL and tested for interference. No interference with was observed.
  
  SPECIES REACTIVITY This kit recognizes human TNF alpha protein. Other species reactivity was determined by measuring 1000 pg/mL recombinant proteins of various species, interpolating the protein concentrations from the human standard curve, and pressing the interpolated concentrations as a percentage of the protein concentration assayed at the same dilution. Reactive species: Primate
  Reactivity < 3% was determined for the following species: Mouse/Rat

  
  

•  人TNF alpha ELISA试剂盒(ab181421)

• CALIBRATION This immunoassay is calibrated against a highly purified human TNF alpha. The NIBSC/WHO unclassified purified human TNF alpha preparation 12/154 was evaluated in this kit. The dose response curve of the unclassified standard TNF alpha parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human TNF alpha kit to approximate NIBSC 12/154 International units, use the equation below.

  
  

  NIBSC (12/154) approximate value (IU/mL) = 0.094 x SimpleStep Human TNF alpha value (pg/mL).

• 说明

  TNF-alpha, also known as cachectin or TNFSF1A, is the prototypic ligand of the TNF superfamily which plays a central role in inflammation, apoptosis, proliferation, invasion, angiogenesis, metastasis and morphogenesis. It is pressed on macrophages, endothelial, epithelial and tumor cells as a 26kDa transmembrane protein. TNF-alpha is cleaved by proteolytic processing into six chains: (1) TNF membrane form, (2) Intracellular domain 1, (3) Intracellular domain 2, (4) C-domain 1, (5) C-domain 2 and (6) TNF soluble form. Signaling from TNF-alpha differs depending on the type of ligand initiating the signaling event (intracellular, membrane or soluble). As an ample, the membrane form of TNF-alpha appears to mediate anti-tumorigenic therapeutic responses whereas the soluble ligand is linked to inflammation and proliferation.