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Bicine Buffer grade（N,N-双（2-羟乙基）甘氨酸）
Bicine Buffer grade（N,N-双（2-羟乙基）甘氨酸）
价 格：询价
产 地：德国更新时间：2025/10/14 11:19:37
品 牌：AppliChem型 号：A1024
状 态：正常点击量：2183
联 系 人：方丽初
电 话：010-88597838
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等 级： (第 19年)
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A1024
| Order No. |
Quantity |
| A1024,0100 |
100 |
g |
| A1024,0250 |
250 |
g |
| A1024,0500 |
500 |
g |
| A1024,1000 |
1 |
kg |
产品参数
| Synonym |
N,N-Bis-(2-hydroxyethyl)-glycine |
| Solubility (20°C) |
100 g/L (H2O) |
| useful pH range |
pH 7.6 - 9.0 |
| pKa (20°C) |
8.35 |
| Melting range |
185 - 192°C |
| Formula |
C6H13NO4 |
| M |
163.17 g/mol |
| CAS-No. |
150-25-4 |
| HS-No. |
29225000 |
| EC-No. |
205-755-1 |
| Storage |
RT |
| LGK |
10 - 13 |
| Disposal |
4 |
| WGK |
1 |
| Specification |
|
| Assay (titr.) |
min. 99 % |
| pH (1 %; H2O; 20°C) |
4.0 - 5.0 |
| Heavy metals (as Pb) |
max. 0.0005 % |
| Water |
max. 1 % |
| A (1 cm/0.1M in H2O) |
|
| 260 nm |
max. 0.08 |
| 280 nm |
max. 0.05 |
产品介绍
(1) Good, N.E. et al. (1966) Biochemistry 5, 467-477
Hydrogen ion buffers for biological research.
(2) Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68
Hydrogen ion buffers.
(3) Werb, Z. et al. (1974) Biochem. J. 139, 359-368
Interaction of α2-Macroglobulin with Proteinases.
(4) Vodicka, P. et al. (1995) Carcinogenesis 16, 1473-1481
DNA damage by styrene.
(5) Eide, I. et al. (1995) Carcinogenesis 16, 1603-1609
Uptake of hemoglobin and DNA adducts after inhalation of C2-C8 1-Alkanes (Olefines) in rats.
(6) Peterkofsky, A. et al. (1995) Biochemistry 34, 8950-8959
The E. coli adenylate cyclase complex: Requirements of the PTS protein for the stimulation by nucleotides.
Bicine is a versatile buffer. Its applications last from enzyme reaction buffers (T4 polynucleotide kinase ref. 4; Micrococcus nuclease ref. 5; adenylate cyclase ref. 6) to electrophoresis buffers (3). Bicine interferes with the BCA and Lowry protein assay. Ferricyanide slowly oxidizes bicine. In addition, its strong binding of copper ions may be disturbing. The working concentration lasts from 3 to 100 mM.
