APMSF hydrochloride BioChemica

A0998

Order No.	Quantity
A0998,0010	10	mg
A0998,0050	50	mg
A0998,0100	100	mg

产品参数

Synonym	4-Amidinophenylmethanesulfonyl fluoride hydrochloride, p-APMSF
Melting point	190 - 192°C (dec.)
Formula	C₈H₉FN₂O₂S · HCl
M	252.69 g/mol
CAS-No.	74938-88-8
HS-No.	29252900
Storage	-20°C
LGK	10 - 13
Specification
Assay (HPLC)	min. 97 %

产品介绍

(1) Laura, R. et al. (1980) Biochemistry 19, 4859-4864
APMSF, ein irreversibler Inhibitor von Serin-Proteasen.
(2) Sueyoshi, T. et al. (1995) Mol. Cell. Biol. 15, 4158-4166
Untersuchung von ''Nuclear Factor NF2d9''.

Hinweis

APMSF is an irreversible inhibitor of plasma serine proteases (trypsin-like proteases), which are specific for the basic amino acid side chains lysine and arginine. If applied in equimolar ratios of enzyme and inhibitor, immediate, complete inhibition of trypsin and thrombin occurs. At a 5 to 10fold excess of inhibitor, factor Xa (bovine) and plasmin (human) will be inhibited. Acetylcholine esterase is insensitive.
APMSF is a more potent inhibitor than PMSF. Its effective concentration is 10 - 50 μM or 2.5 μg/ml, respectively (e. g. ref. 2). Stock solutions are prepared in water at concentrations of 1 - 5 mM. It is stable, when stored aliquoted at -20°C.