百萤课堂之荧光素-dT亚磷酰胺

1.荧光素-dT亚磷酰胺基本参数

CAS             289712-99-8

Ex(nm)          498

Em(nm)          517

分 子 量        1425.58

溶    剂        MeCN

存储条件        保存(＜-15℃)，避免光照

保 质 期        6个月

2.荧光素-dT亚磷酰胺概述

荧光素-dT 亚磷酰胺含有一个 DMT 基团，可以对偶联进行量化。 它可以作为 dT 残基的替代物插入到目标序列中。 它可以将荧光素标签添加到寡核苷酸序列的所需位置。 它与常用的 6-FAM 亚磷酰胺互补，不含 DMT 基团，只能在 5'-末端添加，从而终止合成。我们还提供 HEX-dT 亚磷酰胺、TET-dT 亚磷酰胺、生物素-dT 亚磷酰胺、生物素-dT CPG 和荧光素-dT CPG。

                                      图1.荧光素dT亚磷酰胺的化学结构

3.荧光素-dT亚磷酰胺光谱特性

吸光度(nm)         487

校正因子(260nm)    0.32

校正因子(280nm)    0.35

消光系数(cm^-1M^-1)  80000¹ 

Ex(nm)             498

Em(nm)             517

量子产率           0.7900¹，0.95²

                                         图2.荧光素-dT亚磷酰胺光谱图

4.荧光素-dT亚磷酰胺参考文献

A Chiral-Nanoassemblies-Enabled Strategy for Simultaneously Profiling Surface Glycoprotein and MicroRNA in Living Cells.
Authors: Ma, Wei and Sun, Maozhong and Fu, Pan and Li, Si and Xu, Liguang and Kuang, Hua and Xu, Chuanlai
Journal: Advanced materials (Deerfield Beach, Fla.) (2017)

Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.
Authors: Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun
Journal: Journal of nanoscience and nanotechnology (2011): 7587-92

Identification of unexpected modifications of fluorescein-labeled oligodeoxynucleotides by nuclease P1 digestion and mass spectrometric techniques.
Authors: Wu, H and Skrzypczynski, Z and Cornwell, M J and Aboleneen, H
Journal: Rapid communications in mass spectrometry : RCM (2000): 26-32

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
Authors: Ramírez-Vick, J E and García, A A and Lee, J
Journal: Preparative biochemistry & biotechnology (1998): 243-60