脂肪酶测试盒

产品名称 : 脂肪酶测试盒 | QuantiChrom™ Lipase Assay Kit

分类名称 : 酶活性测定,血液尿液化学

产品介绍 : 测定方法  比色法（412nm）定量测定脂肪酶活性。

产品说明  脂肪酶可催化水解脂类中甘油主链上的酯键。在人体消化系统中分解脂肪的关键酶是胰脂肪酶，它可将脂肪中甘油三酯分解为甘油一酯与游离脂肪酸。人体的胰脂肪酶和与之相关的蛋白2，主要是由胰腺分泌的。急性胰腺炎发作时，脂肪酶含量可在24到48小时内上升5到10倍。脂肪酶活性的增强可能与胰管阻塞，胰腺癌，肾脏疾病，唾液腺炎症，肠梗阻和其他胰腺疾病等有关。脂肪酶活性不足则可能是因为胰腺中分泌脂肪酶的细胞遭受了永久性的损伤。方便、直接、自动化的脂肪酶活性检测手段是非常理想的。本公司研制的脂肪酶检测试剂盒使用改良试剂，通过脂肪酶裂解生成黄色物质，在波长为412nm下检测，其颜色强度与样本的酶活性成正比。

产品用途  直接检测血清、血浆、唾液，尿液以及其他生物样本中的脂肪酶活性。

产品特点  敏感、准确：96孔板测试中，其检测范围在40-1600 U/L。简便、高通量：整个检测过程只需加入一种工作试剂，在室温或37℃下，分别于10分钟和20分钟时读取吸光度即可。每天可自动测试上千份样本。

试剂盒规格  15 mL 缓冲液, 530 mg 显色剂，1 mL 试剂, 2 mL 校准液.

储藏条件  可在室温下运输。将所有试剂置于4℃环境下保存，保质期6个月.

DESCRIPTION  LIPASE catalyzes the hydrolysis of ester bonds on the glycerol backbone of a lipid substrate. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. Human pancreatic lipase and its related protein 2 are the main lipases secreted by the pancreas. In acute pancreatitis, lipase levels can rise 5 to 10-fold within 24 to 48 hours. Increased activities have also been associated with pancreatic duct obstruction, pancreatic cancer, kidney disease, salivary gland inflammation, bowel obstruction, and other pancreatic diseases. Decreased levels may indicate permanent damage to lipase-producing cells in the pancreas. Simple, direct and automation-ready procedures for measuring lipase activity are very desirable. Our Lipase Assay is based on an improved reagent to form a yellow colored product. The color intensity, measured at 412 nm, is proportionate to the enzyme activity in the sample.

APPLICATIONS  Direct assays of lipase activity in serum, plasma, saliva, urine and other biological samples.

KEY FEATURES  Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay. Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day.

KIT CONTENTS  15 mL Assay Buffer, 530 mg Color Reagent, 1 mL Reagent, 2 mL Calibrator.

STORAGE CONDITIONS This product is shipped at room temperature. Store all components at 4°C. Shelf life of at least 6 months.