热门词：生物显微镜 水质分析仪 微波消解 荧光定量PCR 电化学工作站 生物安全柜
产品名称 : 激酶测试盒 | EnzyChrom™ Kinase Assay Kit
分类名称 : 高效药物筛选
产品介绍 : 测定方法 快速荧光法（530nm/590nm）测定激酶的活性和高通量筛选激酶的抑制剂。
产品说明 激酶，又名磷酸转移酶，是酶类的一个大家族，能将磷酸基从高能供体分子ATP上转移到特定底物中。已知激酶参与调节大多数的细胞学过程。该家族的最主要成员是蛋白激酶。到目前为止，已经在人体中发现了518种激酶，高达30%的人体蛋白质都是由这些激酶调节的。激酶的巨大多样性及其在细胞信号转导中的重要作用，使其成为药物研发的理想靶点。本公司生产的激酶测试盒为检测激酶活性以及高通量筛选激酶抑制剂提供了一种简单、快捷的方法。这种基于微孔板的匀相检测法只需用一种工作试剂来培养激酶，通过酶解反应ADP将转化成ATP和丙酮酸盐，再采用荧光（530nm/590nm）检测法对其进行定量分析。
产品特点 安全：非放射性检测法。灵敏且准确：定量范围低至0.01 U/L激酶。匀相且简便：“混合-培养-测定”型检测法。整个检测过程只需加入一种工作试剂，并在室温下培养10分钟。稳定且适合高通量筛选：检测可耐受高达300 mM浓度的ATP和10%的二甲亚砜（DMSO）。采用96/384孔板检测，Z’系数通常> 0.6。采用高通量筛选液体处理系统易于自动化，每天可处理成千上万的样品。
适用范围 激酶活性的检测及激酶抑制剂的高通量筛选。ADP 测定：细胞和其它生物样品中的ADP浓度。
试剂盒规格
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试剂 A: 10 mL |
试剂 B: 10 mL |
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缓冲液: 25 mL |
标准品: 100 mL 3 mM ADP
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储存条件：所有试剂均在-20°C下保存，该品应在冰上运输。保质期至少6个月。
Description kinaseS, also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug developments.our EnzyChromTM Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorimetric (530nm/590nm) assay method.
Key features Safe. Non-radioactive assay.Sensitive and accurate. As low as 0.01 U/L kinase can be quantified.Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature.Robust and amenable to HTS: Assay can tolerate up to 300 mM ATP and 10% dimethylsulfoxide (DMSO). Z’ factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day.
Applications Kinase activity assays and HTS for kinase inhibitors.ADP determination in cells and other biological samples.
Kit Contents
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Reagent A: 10 mL |
Reagent B: 10 mL |
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Assay Buffer: 25 mL |
Standard: 100 mL 3 mM ADP
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Storage conditions: store all reagents at -20°C. This product is shipped on dry ice. Shelf life of at least 6 months.
