MQR1.602 anti-modified mitrulline (clone 4CS)
产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQR1.602
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQR1.602
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)品名
单克隆小鼠抗修饰瓜氨酸
(AMC),免疫球蛋白,克隆C4S
货号。
MQR1.602-100号
批号。
13267个
数量
100微克
版本:2014年8月6日
第1页,共1页
预期用途
本产品仅供研究使用。不用于
诊断或治疗程序。
该产品经测试可用于酶联免疫吸附
ELISA法和Western Blot法。
提供试剂
抗体以PBS形式提供。
同型
小鼠IgG2a,k
免疫原
2,3修饰瓜氨酸肽-
丁二酮单肟和安替比林。
特异性
特异性已在ELISA(图1)和WB(图
2) 是的。识别经修饰的瓜氨酸蛋白
与2,3-丁二酮单肟和安替比林无关
邻近的氨基酸序列。
纯度
纯化蛋白A。
注意事项
一。对于专业用户。
2。就像从生物来源得到的任何产品一样,
应采用适当的处理程序。
三。该产品可用于不同的技术和
结合不同的样品类型和材料,
因此,每个实验室都应验证
应用测试系统。
抗体的制备
使用提供的抗体。
存储说明
储存于-80°C。
应用指南
酶联免疫吸附试验:0.08–0.5微克/毫升
白细胞:1微克/毫升
除非实际测试系统的稳定性
已确定,建议稀释产品
立即使用。
关联
此抗体用于检测瓜氨酸
含有蛋白质的。氨基酸瓜氨酸是由
精氨酸的翻译后修饰
肽基精氨酸脱氨酶(PADs;MQ16.201和
MQ16.203)。抗瓜氨酸抗体
类风湿中检测到蛋白质(如组蛋白)
关节炎患者。
图1:AMC免疫球蛋白(MQR1.602)的特异性,通过酶联免疫吸附试验测定。
在含有0.05%吐温-20和1%牛血清白蛋白的PBS中稀释至0.4微克/毫升的抗体
不同修饰的瓜氨酸肽及其未修饰肽的检测
Product Name
Monoclonal Mouse Anti-Modified Citrulline
(AMC), Immunoglobulin, clone C4S
CAT No.
MQR1.602-100
LOT No.
13267
Quantity
100 μg
Edition: August 6, 2014
Page 1 of 1
Intended use
This product is for research use only. NOT for use in
diagnostic or therapeutic procedures.
This product is tested for use in enzyme-linked immunosorbent
assay (ELISA) and Western Blot (WB).
Reagent provided
The antibody is supplied in PBS.
Isotype
Mouse IgG2a, κ
Immunogen
Citrulline-containing peptide modified with 2,3-
butanedione monoxime and antipyrine.
Specificity
Specificity has been tested in ELISA (figure 1) and WB (figure
2). Recognizes citrulline-containing proteins modified
with 2,3-butanedione monoxime and antipyrine regardless
of amino acid sequences.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources,
proper handling procedures should be used.
3. The product may be used in different techniques and in
combination with different sample types and materials,
therefore each individual laboratory should validate
the applied test system.
Preparation of the antibody
Use antibody as supplied.
Storage instructions
Store at -80°C.
Application guidelines
ELISA: 0.08 – 0.5 μg/ml
WB: 1 μg/ml
Unless the stability in the actual test system has been
established, it is recommended to dilute the product
immediately before use.
Relevance
This antibody is developed for the detection of citrulline
containing proteins. The amino acid citrulline is generated by
posttranslational modification of arginine by
peptidylarginine-deiminases (PADs; MQ16.201 and
MQ16.203). Antibodies directed to citrulline containing
proteins (e.g. histones) are detected in rheumatoid
arthritis patients.
Figure 1: Specificity of AMC Immunoglobulin (MQR1.602), determined by ELISA.
Antibody diluted to 0.4 μg/ml in PBS containing 0.05% tween-20 and 1% BSA was
tested on various modified citrulline-containing peptides and their non-modified