MQ1.201 anti-RPP20 (clone 1F11)
产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ1.201
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ1.201
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)品名
单克隆小鼠
抗hRPP20免疫球蛋白,克隆1F11
货号。
MQ 1.201-100号
批号。
TD1.201-0410-06号
数量
100微克
版本:2014年2月5日
预期用途
本产品仅供研究使用。不用于
诊断或治疗程序。
该产品经测试可用于酶联免疫吸附
检测(ELISA)、免疫印迹(IB)、免疫沉淀(IP)
或免疫荧光抗体分析(IFA)
提供试剂
抗体已在10毫米铵中冻干
碳酸氢盐缓冲液。每小瓶含2毫克牛血清白蛋白。
同型
小鼠IgG2a
免疫原
重组GST-hRPP20(NCBI加入号
AAC24113,表达载体pGEX-2T),在大肠杆菌中表达。
特异性
特异性已在ELISA中测试(图1)。在
免疫印迹法,在不太严格的洗涤条件下
还检测到约60kDa的弱带(图
2,右侧面板)。交叉反应性的附加试验尚未完成
已经完成了。
纯度
纯化蛋白A。
注意事项
一。对于专业用户。
2。就像从生物来源得到的任何产品一样,
应采用适当的处理程序。
三。该产品可用于不同的技术和
结合不同的样品类型和材料,
因此,每个实验室应验证
应用测试系统。
抗体的制备
?推荐抗体浓度:0.5 mg/ml
(当以0.5 mg/ml溶解时,BSA浓度将
为1%)。
?推荐溶剂;100 mM PBS或Tris HCl,pH 7.0
?建议添加叠氮化钠(高达0.05%)
用于长期储存。
?对于1%BSA中0.5 mg/ml抗体浓度,
溶于200微升缓冲液中。
注意:小心打开小瓶,因为抗体存在于
真空。
存储说明
溶解抗体并在2-8℃下保存。
稀释指南
酶联免疫吸附试验:1:(4000×F)–1:(6000×F)。
IB:1:(500×F)预测分子量:20kda。
其他应用程序:由于应用程序不同,您应该
确定产品的最佳工作稀释度
适合你的特殊需要。
有关倍增因子F的值,请参见小瓶上的标签。
除非实际测试系统的稳定性
已确定,建议稀释产品
立即使用。
关联
真核细胞的细胞核含有几类
RNA蛋白质复合物。小核
核糖核蛋白)参与前mRNAs的剪接
内含子切除)在核质中。RPP20是这样一个
复杂。SnoRNPs(小核仁核糖核蛋白)是
参与核仁中前核糖体RNA的成熟。
预rRNA处理和修改需要
snoRNA及其蛋白质成分(如Rpp20)
复合物。通常,snornp包含许多常见的
蛋白质,与其他同类型的鼻息共享
家族,在几个颗粒特异性蛋白质旁边。平均来说,
snoRNPs含有约6-10个蛋白质亚单位。
0个
0,2个
0,4个
0,6个
0,8个
1个
1,2个
1,4个
hRpp20 GST hRpp14 GST hRrp46-HIS10 GST BSA
抗原OD450
抗体
品名
单克隆小鼠
抗hRPP20免疫球蛋白,克隆1F11
货号。
MQ 1.201-100号
批号。
TD1.201-0410-06号
大小
100微克
版本:2014年2月5日
Product Name
Monoclonal Mouse
Anti-hRPP20 Immunoglobulin, clone 1F11
CAT No.
MQ 1.201-100
LOT No.
TD1.201-0410-06
Quantity
100 μg
Edition: February 5, 2014
Intended use
This product is for research use only. NOT for use in
diagnostic or therapeutic procedures.
This product is tested for use in enzyme-linked immunosorbent
assay (ELISA), immunoblotting (IB), immunoprecipitation (IP)
or immunofluorescent antibody analysis (IFA)
Reagent provided
The antibody has been lyophilized in a 10 mM ammonium
bicarbonate buffer. Each vial contains 2 mg BSA.
Isotype
Mouse IgG2a
Immunogen
Recombinant GST-hRPP20 (NCBI accession number
AAC24113, expression vector pGEX-2T), expressed in E.coli.
Specificity
Specificity has been tested in ELISA (figure 1). In
immunoblotting, under less stringent washing conditions, a
weaker band of approximately 60 kDa is also detected (figure
2, right panel). Additional tests for cross reactivity have not yet
been performed.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources,
proper handling procedures should be used.
3. The product may be used in different techniques and in
combination with different sample types and materials,
therefore each individual laboratory should validate the
applied test system.
Preparation of the antibody
? Recommended antibody concentration: 0.5 mg/ml
(when dissolved at 0.5 mg/ml, the BSA concentration will
be 1%).
? Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0
? Additional sodium azide (up to 0.05%) is recommended
for long term storage.
? For a 0.5 mg/ml antibody concentration in 1% BSA,
dissolve in 200 μl buffer.
NOTE: Be careful opening the vial since the antibody resides in a
vacuum.
Storage instructions
Dissolve the antibody and store at 2-8oC.
Dilution guidelines
ELISA: 1:(4000 x F) – 1:(6000 x F).
IB: 1:(500 x F) predicted molecular weight: 20 kDa.
Other applications: since applications vary, you should
determine the optimum working dilution of the product that
is appropriate for your specific need.
For the value of the multiplication factor F, see label on vial.
Unless the stability in the actual test system has been
established, it is recommended to dilute the product
immediately before use.
Relevance
The nuclei of eukaryotic cells contain several classes of small
RNA-protein complexes. SnRNPs (small nuclear
ribonucleoproteins) are involved in splicing of pre-mRNAs (the
excision of introns) in the nucleoplasm. RPP20 is part of such a
complex. SnoRNPs (small nucleolar ribonucleoproteins) are
involved in the maturation of pre-ribosomal RNA in the nucleoli.
Pre-rRNA processing and modification require both the
snoRNA and the protein constituents (such as Rpp20) of these
complexes. Generally, snoRNPs contain a number of common
proteins, which are shared with other snoRNPs of the same
family, next to several particle-specific proteins. On average,
snoRNPs contain about 6-10 protein subunits.
0
0,2
0,4
0,6
0,8
1
1,2
1,4
hRpp20-GST hRpp14-GST hRrp46-HIS10 GST BSA
Antigen OD450
Antibody
Product Name
Monoclonal Mouse
Anti-hRPP20 Immunoglobulin, clone 1F11
CAT No.
MQ 1.201-100
LOT No.
TD1.201-0410-06
Size
100 μg
Edition: February 5, 2014