MQ2.103 anti-RRP4 (Clone 15B3)
产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ2.103
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ2.103
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)品名
单克隆小鼠
抗hRRP4免疫球蛋白,克隆15B3
货号。
MQ 2.103-100标准
很多。不。
TD2.103-0410-06号
数量
100微克
2012年2月1日版
预期用途
本产品仅供研究使用。不用于
诊断或治疗程序。
该产品经测试可用于酶联免疫吸附
检测(ELISA)、免疫印迹(IB)或免疫沉淀
(知识产权)。
提供试剂
抗体已在10毫米铵中冻干
碳酸氢盐缓冲液。每小瓶含2毫克牛血清白蛋白。
同型
小鼠IgG1
免疫原
重组GST-hRRP4(NCBI加入号
nmu 014285区域3-884,表达载体pGEX-4T3,
在大肠杆菌中表达。
RRP4的同义词;p7,EXOSC2,Rrp4p
特异性
特异性已在ELISA中测试(图1)。附加试验
因为交叉反应还没有进行。
纯度
纯化蛋白A。
注意事项
一。对于专业用户。
2。就像从生物来源得到的任何产品一样,
应采用适当的处理程序。
三。该产品可用于不同的技术和
结合不同的样品类型和材料,
因此,每个实验室应验证
应用测试系统。
抗体的制备
?推荐抗体浓度:0.5 mg/ml
(当以0.5 mg/ml溶解时,BSA浓度将
为1%)。
?推荐溶剂;100 mM PBS或Tris HCl,pH 7.0
?建议添加叠氮化钠(高达0.05%)
用于长期储存。
?对于1%BSA中0.5 mg/ml抗体浓度,
溶于200微升缓冲液中。
注意:小心打开小瓶,因为抗体存在于
真空。
存储说明
溶解抗体并在2-8℃下保存。
稀释指南
酶联免疫吸附试验:1:(4000×F)–1:(6000×F)。
IB:1:(500华氏度)。预测分子量:33kDa。
其他应用程序:由于应用程序不同,您应该
确定产品的最佳工作稀释度
适合你的特殊需要。
有关倍增因子F的值,请参见小瓶上的标签。
除非实际测试系统的稳定性
已确定,建议稀释产品
立即使用。
关联
胞外体,存在于细胞核和细胞质中
真核细胞是3'-5'外核糖核酸酶的复合物
至少包含九个核心组件。最近
主要通过酵母的分析证明
外显子是rRNA处理和sn(o)RNA的关键
生物成因。此外,它还参与了
处理不当的mrna。胞质外体
参与正常的mRNA转换和
含有富AU元素的固有不稳定mrna。
因此,外显子在RNA代谢中起着关键作用。
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hRpp20 GST hRrp4-HIS6牛血清白蛋白
Product Name
Monoclonal Mouse
Anti-hRRP4 Immunoglobulin, clone 15B3
CAT No.
MQ 2.103-100
LOT. No.
TD2.103-0410-06
Quantity
100 μg
Edition February 1, 2012
Intended use
This product is for research use only. NOT for use in
diagnostic or therapeutic procedures.
This product is tested for use in enzyme-linked immunosorbent
assay (ELISA), immunoblotting (IB) or immunoprecipitation
(IP).
Reagent provided
The antibody has been lyophilized in a 10 mM ammonium
bicarbonate buffer. Each vial contains 2 mg BSA.
Isotype
Mouse IgG1
Immunogen
Recombinant GST-hRRP4 (NCBI accession number
NM_014285 REGION 3-884, expression vector pGEX-4T3),
expressed in E.coli.
Synonyms for RRP4; p7, EXOSC2, Rrp4p
Specificity
Specificity has been tested in ELISA (figure 1). Additional tests
for cross reactivity have not yet been performed.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources,
proper handling procedures should be used.
3. The product may be used in different techniques and in
combination with different sample types and materials,
therefore each individual laboratory should validate the
applied test system.
Preparation of the antibody
? Recommended antibody concentration: 0.5 mg/ml
(when dissolved at 0.5 mg/ml, the BSA concentration will
be 1%).
? Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0
? Additional sodium azide ( up to 0.05%) is recommended
for long term storage.
? For a 0.5 mg/ml antibody concentration in 1% BSA,
dissolve in 200 μl buffer.
NOTE: Be careful opening the vial since the antibody resides in a
vacuum.
Storage instructions
Dissolve the antibody and store at 2-8oC.
Dilution guidelines
ELISA: 1:(4000 x F) – 1:(6000 x F).
IB: 1:(500 x F). Predicted molecular weight: 33 kDa.
Other applications: since applications vary, you should
determine the optimum working dilution of the product that
is appropriate for your specific need.
For the value of the multiplication factor F, see label on vial.
Unless the stability in the actual test system has been
established, it is recommended to dilute the product
immediately before use.
Relevance
The exosome, present in both the nucleus and cytoplasm of all
eukaryotic cells, is a complex of 3'-5' exoribonucleases
containing at least nine core components. Recently, it has been
demonstrated, mainly by analyses in yeast, that the nuclear
exosome is essential for rRNA processing and sn(o)RNA
biogenesis. Furthermore, it is involved in the degradation of
improperly processed mRNAs. The cytoplasmic exosome
participates in normal mRNA turnover and in the degradation of
inherently instable mRNAs that contain AU-rich elements.
Therefore, the exosome plays a key role in RNA metabolism.
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hRpp20-GST hRrp4-HIS6 BSA