MQ13.101 anti-citrullinated fibrinogen (clone 3D1)
产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ13.101
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ13.101
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)品名
抗瓜氨酸纤维蛋白原单克隆小鼠
免疫球蛋白,3D1克隆
货号。
MQ13.101-100号
批号。
13252个
大小
100微克
版本:2020年1月15日
预期用途
本产品仅供研究使用。不用于诊断或
治疗程序。
来自免疫精确抗体(欧洲)B.V.的许可证
需要在研究领域之外使用。
该产品经测试可用于酶联免疫吸附试验
(ELISA)、免疫印迹(IB)或免疫组织化学(IHC)。
提供试剂
抗体已在10毫米碳酸氢铵中冻干
缓冲区。
同型
小鼠IgG1
免疫原
去离子小鼠纤维蛋白原肽。
特异性
特异性已在ELISA中测试(图1)。
与去离子的人纤维蛋白原发生交叉反应。
交叉反应性的附加试验尚未进行。
纯度
纯化蛋白A。
注意事项
一。对于专业用户。
2。与任何来自生物源的产品一样
应使用处理程序。
三。该产品可用于不同的技术和
结合不同的样品类型和材料,
因此,每个实验室应验证
应用测试系统。
抗体制备
?推荐抗体浓度:0.5 mg/ml
?推荐溶剂;PBS或100毫米Tris HCl,pH 7.0
?建议长期添加叠氮化钠(高达0.05%)
定期储存。
?对于0.5 mg/ml抗体浓度,溶于200微升
缓冲区。
注意:小心打开小瓶,因为抗体存在于
真空。
存储说明
对于长期储存,将冻干批次保持在-20°C
溶解后在2-8°C下储存。为了延长储存时间,添加叠氮化钠
至0.05%
稀释指南
酶联免疫吸附试验:1:2000–1:4000。
IB:1:100–1:500。
IHC:1:100–1:500。
其他应用程序:由于应用程序不同,您应该确定
适合您的产品的最佳工作稀释度
具体需要。
关联
纤维蛋白原是肝脏产生的一种蛋白质,有助于止血
帮助血栓形成。
纤维蛋白原通过
炎症关节中的肽基精氨酸脱氨酶
患类风湿性关节炎。
瓜氨酸虽然是一种氨基酸,但在
蛋白质合成,因为它不是由DNA编码的,但是有几种蛋白质是
已知含有瓜氨酸。通常含有瓜氨酸的蛋白质
残余物包括髓鞘碱性蛋白(MBP)、丝蛋白和一些
组蛋白,而其他蛋白质,如纤维蛋白和波形蛋白可以得到
在细胞死亡和组织炎症时被清除。
类风湿关节炎患者通常(至少80%)会发展成
对含有瓜氨酸的蛋白质的免疫反应。尽管
这种免疫反应的起源尚不清楚,检测到
与含有瓜氨酸的蛋白质或肽反应的抗体是
现在成为诊断类风湿的重要帮助
关节炎。
10个
-3 10个
-210个
-110个
0 10个
10.0分
0.5分
1.0条
1.5瓜氨酸纤维蛋白原的检测
浓度抗体(μg/ml)
450nm处外径
图1:抗去离子小鼠纤维蛋白原免疫球蛋白3D1克隆的特异性
(MQ13.101),用ELISA测定。在含有0.05%吐温-20和1%的PBS中稀释的抗体
在兔PAD2(sigma;cat no P4874)脱氨小鼠纤维蛋白原上检测BSA
Product Name
Monoclonal Mouse anti-Citrullinated Fibrinogen
Immunoglobulin, clone 3D1
CAT No.
MQ13.101-100
LOT No.
13252
Size
100 μg
Edition: January 15, 2020
Intended use
This product is for research use only. NOT for use in diagnostic or
therapeutic procedures.
A license from ImmunoPrecise Antibodies (Europe) B.V. is
required for use outside the research field.
This product is tested for use in enzyme-linked immunosorbent assay
(ELISA), immunoblotting (IB), or Immunohistochemistry (IHC).
Reagent provided
The antibody has been lyophilized in a 10 mM ammonium bicarbonate
buffer.
Isotype
Mouse IgG1
Immunogen
Deiminated murine fibrinogen peptide.
Specificity
Specificity has been tested in ELISA (figure 1).
Crossreacts with deiminated human fibrinogen.
Additional tests for cross reactivity have not yet been performed.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources, proper
handling procedures should be used.
3. The product may be used in different techniques and in
combination with different sample types and materials,
therefore each individual laboratory should validate the
applied test system.
Preparation ofthe antibody
? Recommended antibody concentration: 0.5 mg/ml
? Recommended solvent; PBS or 100mM Tris-HCl, pH 7.0
? Additional sodium azide (up to 0.05%) is recommended for long
term storage.
? For a 0.5 mg/ml antibody concentration, dissolve in 200 μl
buffer.
NOTE: Be careful opening the vial since the antibody resides in a
vacuum.
Storage instructions
For long term storage keep lyophilized batch at -20°C
After dissolving store at 2-8°C. For prolonged storage add sodium azide
to 0.05%
Dilution guidelines
ELISA: 1:2000 – 1:4000.
IB: 1:100 – 1:500.
IHC: 1:100 – 1:500.
Other applications: since applications vary, you should determine the
optimum working dilution of the product that is appropriate for your
specific need.
Relevance
Fibrinogen is a protein produced by the liver which helps stop bleeding
by helping blood clots to form.
Fibrinogen gets deiminated (conversion from arginin to citrullin) by
Peptidyl Arginine Deiminase (PAD) in inflamed joints in patients that
develop rheumatoid arthritis.
Citrulline, while being an amino acid, is not built into proteins during
protein synthesis, as it is not coded for by DNA, yet several proteins are
known to contain citrulline. Proteins that normally contain citrulline
residues include myelin basic protein (MBP), filaggrin, and several
histone proteins, while other proteins, like fibrin and vimentin can get
deiminated during cell death and tissue inflammation.
Patients with rheumatoid arthritis often (at least 80% of them) develop
an immune response against proteins containing citrulline. Although
the origin of this immune response is not known, detection of
antibodies reactive with citrulline containing proteins or peptides is
now becoming an important help in the diagnosis of rheumatoid
arthritis.
10
-3 10
-2 10
-1 10
0 10
1 0.0
0.5
1.0
1.5Detection of citrullinated fibrinogen
Concentration antibody (μg/ml)
OD at 450nm
Figure 1: Specificity of Anti-Deiminated Murine fibrinogen Immunoglobulin, clone 3D1
(MQ13.101), determined by ELISA. Antibody diluted in PBS containing 0.05% tween-20 and 1%
BSA was tested on rabbit PAD2 (sigma; cat no P4874) deiminated murine fibrinogen