MQ10.101 anti-PTPRR isoforms (clone 6A6)
产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ10.101
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2023/4/3 18:11:18
产地:美国
品牌:ImmunoPrecise
型号:MQ10.101
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)品名
单克隆小鼠
抗PTPRR蛋白亚型(PTPBR7、PTP-SL和
PTPPBS)γ免疫球蛋白,克隆6A6
货号。
MQ 10.101-100标准
批号。
TD10.101-0126-07
数量
100微克
版本:2012年2月1日
预期用途
本产品仅供研究使用。不用于
诊断或治疗程序。
本产品经测试可用于免疫印迹(IB),
免疫沉淀(IP)或免疫组织化学(IHC)
提供试剂
抗体已在10毫米铵中冻干
碳酸氢盐缓冲液。每小瓶含2毫克牛血清白蛋白。
同型
小鼠IgG2a
免疫原
重组PTP-SL-GST(NCBI登录号
BN000437,表达载体pGEX-2T),在大肠杆菌中表达。
特异性
特异性已在免疫印迹中测试(图
1) 是的。与富含纹状体的磷酸酶交叉反应
(步骤)。交叉反应性的附加试验
已经表演过了。
纯度
纯化蛋白A。
注意事项
一。对于专业用户。
2。就像从生物来源得到的任何产品一样,
应采用适当的处理程序。
三。该产品可用于不同的技术和
结合不同的样品类型和材料,
因此,每个实验室应验证
应用测试系统。
抗体的制备
?推荐抗体浓度:0.5 mg/ml
(当以0.5 mg/ml溶解时,BSA浓度将
为1%)。
?推荐溶剂;100 mM PBS或Tris HCl,pH 7.0
?建议添加叠氮化钠(高达0.05%)
用于长期储存。
?对于1%BSA中0.5 mg/ml抗体浓度,
溶于200微升缓冲液中。
注意:小心打开小瓶,因为抗体存在于
真空。
存储说明
溶解抗体并在2-8℃下保存。
稀释指南
产品的最佳工作稀释度尚未确定
决心。
除非实际测试系统的稳定性
已确定,建议稀释
使用前立即使用。
关联
蛋白质酪氨酸磷酸酶(PTPs)是
有助于确定时空平衡
酪氨酸磷酸化与非磷酸化之间
从而协调调节这些细胞反应
细胞外信号。Ptprr基因产生了4种不同的
神经磷酸酶,它们的胞间部分的长度和亚细胞定位不同。PTPBR7(72 kDa)是
受体样,PTP-SL(60kda)与膜相关
PTPPBSγ(42和37kda)是一种胞浆性磷酸酶。这个
抗体直接作用于这些蛋白质的共同部分。
图1:PTP-SL或STEP瞬时转染神经2a细胞
表达质粒,如车道上方所示。模拟转染细胞
作为阴性对照。裂解物直接用西药
用单克隆抗体6A6进行杂交分析。在PTP-SL车道:60 kDa PTP-SL,
在42和37 kDa时,两种PTPPBSγ亚型。
Product Name
Monoclonal Mouse
Anti-PTPRR protein isoforms (PTPBR7, PTP-SL and
PTPPBS)γ Immunoglobulin, clone 6A6
CAT No.
MQ 10.101-100
LOT No.
TD10.101-0126-07
Quantity
100 μg
Edition: February 1, 2012
Intended use
This product is for research use only. NOT for use in
diagnostic or therapeutic procedures.
This product is tested for use in immunoblotting (IB),
immunoprecipitation (IP) or immunohistochemistry (IHC)
Reagent provided
The antibody has been lyophilized in a 10 mM ammonium
bicarbonate buffer. Each vial contains 2 mg BSA.
Isotype
Mouse IgG2a
Immunogen
Recombinant PTP-SL-GST (NCBI accession number
BN000437, expression vector pGEX-2T), expressed in E.coli.
Specificity
Specificity has been tested in immunoblotting (figure
1). Crossreacts with the striatum enriched phosphatase
(STEP). Additional tests for cross reactivity have not
yet been performed.
Purity
Protein A purified.
Precautions
1. For professional users.
2. As with any product derived from biological sources,
proper handling procedures should be used.
3. The product may be used in different techniques and in
combination with different sample types and materials,
therefore each individual laboratory should validate the
applied test system.
Preparation of the antibody
? Recommended antibody concentration: 0.5 mg/ml
(when dissolved at 0.5 mg/ml, the BSA concentration will
be 1%).
? Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0
? Additional sodium azide ( up to 0.05%) is recommended
for long term storage.
? For a 0.5 mg/ml antibody concentration in 1% BSA,
dissolve in 200 μl buffer.
NOTE: Be careful opening the vial since the antibody resides in a
vacuum.
Storage instructions
Dissolve the antibody and store at 2-8oC.
Dilution guidelines
Optimum working dilutions of the product are not yet
determined.
Unless the stability in the actual test system has been
established, it is recommended to dilute the
product immediately before use.
Relevance
Protein tyrosine phosphatases (PTPs) are the enzymes that are
instrumental in determining the spatial and temporal balance
between the tyrosine phosphorylated and non-phosphorylated
targets, and thus coordinately regulate these cellular responses
to extracellular cues. The Ptprr gene gives rise to 4 different
neuronal phosphatases which differ in length of their Nterminal part and subcellular localization. PTPBR7 (72 kDa) is
receptor-like, PTP-SL (60 kDa) is membrane associated and
PTPPBSγ (42 and 37 kDa) is a cytosolic phoaphatase. This
antibody is directed to the common part of these proteins.
Figure 1: Neuro-2a cells were transiently transfected with PTP-SL or STEP
expression plasmids, as indicated above the lanes. Mock transfected cells were
included as negative controls. Lysates were directly subjected to Western
blot analysis using monoclonal antibodies 6A6. In the PTP-SL lane: at 60 kDa PTP-SL,
at 42 and 37 kDa two PTPPBSγ isoforms.