MQR2.2201 anti-SMARCA4

品名

单克隆人抗SWI/SNF相关基质结合肌动蛋白依赖性染色质调节因子

a4亚型B免疫球蛋白

货号。

MQR 2.2201号

批号。

18283年

数量

100微克

版本：2019年9月4日

预期用途

本产品仅供研究使用。不用于诊断或

治疗程序。

该产品经测试可用于酶联免疫吸附试验

（ELISA）和免疫沉淀（IP）。

提供试剂

抗体以PBS形式提供。

同型

人IgG1-κ

免疫原

SWI/SNF相关基质相关肌动蛋白依赖性调节因子

染色质a4亚型B。结构域：1451-1580，1647，溴多胺。

特异性

特异性已在ELISA（图1）和IP-MS中测试。

纯度

纯化蛋白A。

免责声明

该抗体仅供研发使用。不用于诊断或

治疗程序。

注意事项

一。对于专业用户。

2。与任何来自生物源的产品一样

应使用处理程序。

三。该产品可用于不同的技术和

结合不同的样品类型和材料，

因此，每个实验室应验证

应用测试系统。

抗体的制备

使用提供的抗体。

储存/稳定性

储存于-20°C。首次使用后，储存于4°C。避免重复冻融循环。

应用指南

酶联免疫吸附试验：1:1000-1:5000

IP:2微克/毫升

其他用途：由于用途不同，最佳工作稀释度

应在适当的分析中测定产品的含量。

除非实际测试系统的稳定性已经确定，否则

建议在使用前立即稀释产品。

关联

参与选择性基因的转录激活和抑制

染色质重塑（DNA核小体拓扑的改变）。

SWI/SNF染色质重塑复合物的组成

关键酶活性，改变染色质结构

ATP依赖的核小体内的DNA组蛋白接触

态度。CREST-BRG1复合物的成分，一种多蛋白

调节启动子激活的复合物

钙依赖性阻遏复合物的释放和募集

一种激活剂复合物。在静息神经元中，c-FOS的转录

启动子被SMARCA4依赖的

磷酸-RB1-HDAC阻遏复合物。钙内流时，RB1是

钙调磷酸酶脱磷，导致

阻遏复合物。同时，还增加了

通过嵴依赖机制向启动子皱缩

导致转录激活。CREST-BRG1复合物也结合在一起

对NR2B启动子和NR2B的活性依赖性诱导

表达包括HDAC1的释放和CREBBP的募集。

属于神经祖细胞特异性染色质重塑

复合物（npBAF复合物）与神经元特异性染色质

重塑复合体（nBAF复合体）

Product Name

Monoclonal human Anti-SWI/SNF-related matrixassociated actin-dependent regulator of chromatin

a4 isoform B Immunoglobulin

CAT No.

MQR 2.2201

LOT No.

18283

Quantity

100 μg

Edition: September 4, 2019

Intended use

This product is for research use only. NOT for use in diagnostic or

therapeutic procedures.

This product is tested for use in enzyme-linked immunosorbent assay

(ELISA) and immunoprecipitation (IP).

Reagent provided

The antibody is supplied in PBS.

Isotype

Human IgG1κ

Immunogen

SWI/SNF-related matrix-associated actin-dependent regulator of

chromatin a4 isoform B. Domain: 1451-1580 of 1647, bromodomain.

Specificity

Specificity has been tested in ELISA (figure 1) and IP-MS.

Purity

Protein A purified.

Disclaimer

The antibody is for R&D use only. NOT for use in diagnostic or

therapeutic procedures.

Precautions

1. For professional users.

2. As with any product derived from biological sources, proper

handling procedures should be used.

3. The product may be used in different techniques and in

combination with different sample types and materials,

therefore each individual laboratory should validate the

applied test system.

Preparation of the antibody

Use antibody as supplied.

Storage/Stability

Store at -20°C. After first time use, store at 4°C. Avoid repeated freezethaw cycles.

Application guidelines

ELISA: 1:1000 – 1:5000

IP: 2 μg/ml

Other applications: since applications vary, optimum working dilution

of the product should be determined in the appropriate assay.

Unless the stability in the actual test system has been established, it is

recommended to dilute the product immediately before use.

Relevance

Involved in transcriptional activation and repression of select genes by

chromatin remodeling (alteration of DNA-nucleosome topology).

Component of SWI/SNF chromatin remodeling complexes that carry

out key enzymatic activities, changing chromatin structure by altering

DNA-histone contacts within a nucleosome in an ATP-dependent

manner. Component of the CREST-BRG1 complex, a multiprotein

complex that regulates promoter activation by orchestrating the

calcium-dependent release of a repressor complex and the recruitment

of an activator complex. In resting neurons, transcription of the c-FOS

promoter is inhibited by SMARCA4-dependent recruitment of a

phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is

dephosphorylated by calcineurin, which leads to release of the

repressor complex. At the same time, there is increased recruitment of

CREBBP to the promoter by a CREST-dependent mechanism, which

leads to transcriptional activation. The CREST-BRG1 complex also binds

to the NR2B promoter, and activity-dependent induction of NR2B

expression involves the release of HDAC1 and recruitment of CREBBP.

Belongs to the neural progenitors-specific chromatin remodeling

complex (npBAF complex) and the neuron-specific chromatin

remodeling complex (nBAF complex)