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MQR2.2401	anti-DHTKD1
MQR2.2401	anti-DHTKD1
  • MQR2.2401	anti-DHTKD1

MQR2.2401 anti-DHTKD1

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:ImmunoPrecise

号:MQR2.2401

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词: human dehydrogenase E1 and tra   anti-DHTKD1   MQR2.2401   MQR2.2401 anti-DHTKD1   Human IgG1  

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品名


单克隆人抗脱氢酶E1和


含蛋白1的转酮醇酶结构域


免疫球蛋白


货号。


MQR 2.2401号


批号。


18285年


数量


100微克


版本:2019年9月4日


预期用途


本产品仅供研究使用。不用于诊断或


治疗程序。


该产品经测试可用于酶联免疫吸附试验


(ELISA)和免疫沉淀(IP)。


提供试剂


抗体以PBS形式提供。


同型


人IgG1-κ


免疫原


脱氢酶E1和含蛋白1的转酮醇酶结构域。


域名:45-919/919。


特异性


特异性已在ELISA(图1)和IP-MS中测试。


纯度


纯化蛋白A。


免责声明


该抗体仅供研发使用。不用于诊断或


治疗程序。


注意事项


一。对于专业用户。


2。与任何来自生物源的产品一样


应使用处理程序。


三。该产品可用于不同的技术和


结合不同的样品类型和材料,


因此,每个实验室应验证


应用测试系统。


抗体的制备


使用提供的抗体。


储存/稳定性


储存于-20°C。首次使用后,储存于4°C。避免重复冻融循环。


应用指南


酶联免疫吸附试验:1:1000-1:5000


IP:2微克/毫升


其他用途:由于用途不同,最佳工作稀释度


应在适当的分析中测定产品的含量。


除非实际测试系统的稳定性已经确定,否则


建议在使用前立即稀释产品。


关联


2-氧戊二酸脱氢酶复合物催化


2-氧戊二酸转化为琥珀酰辅酶A和二氧化碳。它包含


三种酶组分的多拷贝:2-氧戊二酸


脱氢酶(E1)、二氢脂酰胺琥珀酰转移酶(E2)和


脂酰胺脱氢酶(E3)(按相似性)。1


图1:ELISA测定抗DHTKD1(MQR2.2401)的特异性。抗体储备0.44


在含有0.05%吐温-20和1%牛血清白蛋白的PBS中稀释的mg/ml)在脱氢酶E1上进行试验


以及含有蛋白质1(蓝色)和非靶蛋白(红色)的转酮醇酶结构域




Product Name

Monoclonal Human anti-dehydrogenase E1 and

transketolase domain containing protein 1

Immunoglobulin

CAT No.

MQR 2.2401

LOT No.

18285

Quantity

100 μg

Edition: September 4, 2019

Intended use

This product is for research use only. NOT for use in diagnostic or

therapeutic procedures.

This product is tested for use in enzyme-linked immunosorbent assay

(ELISA) and immunoprecipitation (IP).

Reagent provided

The antibody is supplied in PBS.

Isotype

Human IgG1κ

Immunogen

Dehydrogenase E1 and transketolase domain containing protein 1.

Domain: 45-919 of 919.

Specificity

Specificity has been tested in ELISA (figure 1) and IP-MS.

Purity

Protein A purified.

Disclaimer

The antibody is for R&D use only. NOT for use in diagnostic or

therapeutic procedures.

Precautions

1. For professional users.

2. As with any product derived from biological sources, proper

handling procedures should be used.

3. The product may be used in different techniques and in

combination with different sample types and materials,

therefore each individual laboratory should validate the

applied test system.

Preparation of the antibody

Use antibody as supplied.

Storage/Stability

Store at -20°C. After first time use, store at 4°C. Avoid repeated freezethaw cycles.

Application guidelines

ELISA: 1:1000 – 1:5000

IP: 2 μg/ml

Other applications: since applications vary, optimum working dilution

of the product should be determined in the appropriate assay.

Unless the stability in the actual test system has been established, it is

recommended to dilute the product immediately before use.

Relevance

The 2-oxoglutarate dehydrogenase complex catalyzes the overall

conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains

multiple copies of three enzymatic components: 2-oxoglutarate

dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and

lipoamide dehydrogenase (E3) (By similarity).1

Figure 1: Specificity of anti-DHTKD1 (MQR2.2401), determined by ELISA. Antibody stock 0.44

mg/ml) diluted in PBS containing 0.05% tween-20 and 1% BSA was tested on Dehydrogenase E1

and transketolase domain containing protein 1 (in blue) and non-target protein (in red)