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MQR2.3901	anti-SARS
MQR2.3901	anti-SARS
  • MQR2.3901	anti-SARS

MQR2.3901 anti-SARS

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:ImmunoPrecise

号:MQR2.3901

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词: human anti-seryl-tRNA syntheta   human seryl-tRNA synthetase   anti-SARS   MQR2.3901   MQR2.3901 anti-SARS  

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品名


单克隆人抗seryl-tRNA合成酶


免疫球蛋白


货号。


MQR 2.3901号


批号。


19047年


数量


100微克


版本:2019年9月11日


预期用途


本产品仅供研究使用。不用于诊断或


治疗程序。


该产品经测试可用于酶联免疫吸附试验


(ELISA)和免疫沉淀(IP)。


提供试剂


抗体以PBS形式提供。


同型


人IgG1-κ


免疫原


Seryl-tRNA合成酶。域:514的V2-A514。


特异性


特异性已在ELISA(图1)和IP-MS中测试。


纯度


纯化蛋白A。


免责声明


该抗体仅供研发使用。不用于诊断或


治疗程序。


注意事项


一。对于专业用户。


2。与任何来自生物源的产品一样


应使用处理程序。


三。该产品可用于不同的技术和


结合不同的样品类型和材料,


因此,每个实验室应验证


应用测试系统。


抗体的制备


使用提供的抗体。


储存/稳定性


储存于-20°C。首次使用后,储存于4°C。避免重复冻融循环。


应用指南


酶联免疫吸附试验:1:1000-1:5000


IP:2微克/毫升


其他用途:由于用途不同,最佳工作稀释度


应在适当的分析中测定产品的含量。


除非实际测试系统的稳定性已经确定,否则


建议在使用前立即稀释产品。


关联


催化丝氨酸与tRNA(Ser)的两步反应:


丝氨酸首先被ATP激活形成Ser AMP,然后转移到


tRNA(Ser)的接受端。也可能是氨基酰化物


tRNA(Sec)与丝氨酸形成酰化错误的tRNA L-seryl-tRNA(Sec),


将进一步转化为硒代半胱氨酸tRNA(Sec)。在


与VEGFA核心启动子结合并防止MYC结合


以及MYC的转录激活。招募SIRT2到VEGFA


启动子,促进‘Lys-16’(H4K16)组蛋白H4的脱乙酰化。


因此,抑制VEGFA的产生和血管新生


由VEGFA.1介导


图1:用酶联免疫吸附试验测定抗SARS的特异性(MQR2.3901)。抗体储备1.38 mg/ml)


在含有0.05%吐温-20和1%牛血清白蛋白的PBS中稀释后,对人血清trnasynthase(蓝色)和非靶蛋白(红色)进行测试




Product Name

Monoclonal Human anti-seryl-tRNA synthetase

Immunoglobulin

CAT No.

MQR 2.3901

LOT No.

19047

Quantity

100 μg

Edition: September 11, 2019

Intended use

This product is for research use only. NOT for use in diagnostic or

therapeutic procedures.

This product is tested for use in enzyme-linked immunosorbent assay

(ELISA) and immunoprecipitation (IP).

Reagent provided

The antibody is supplied in PBS.

Isotype

Human IgG1κ

Immunogen

Seryl-tRNA-synthetase. Domain: V2-A514 of 514.

Specificity

Specificity has been tested in ELISA (figure 1) and IP-MS.

Purity

Protein A purified.

Disclaimer

The antibody is for R&D use only. NOT for use in diagnostic or

therapeutic procedures.

Precautions

1. For professional users.

2. As with any product derived from biological sources, proper

handling procedures should be used.

3. The product may be used in different techniques and in

combination with different sample types and materials,

therefore each individual laboratory should validate the

applied test system.

Preparation of the antibody

Use antibody as supplied.

Storage/Stability

Store at -20°C. After first time use, store at 4°C. Avoid repeated freezethaw cycles.

Application guidelines

ELISA: 1:1000 – 1:5000

IP: 2 μg/ml

Other applications: since applications vary, optimum working dilution

of the product should be determined in the appropriate assay.

Unless the stability in the actual test system has been established, it is

recommended to dilute the product immediately before use.

Relevance

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction:

serine is first activated by ATP to form Ser-AMP and then transferred to

the acceptor end of tRNA(Ser). Is probably also able to aminoacylate

tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec),

which will be further converted into selenocysteinyl-tRNA(Sec). In the

nucleus, binds to the VEGFA core promoter and prevents MYC binding

and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA

promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16).

Thereby, inhibits the production of VEGFA and sprouting angiogenesis

mediated by VEGFA.1

Figure 1: Specificity of anti-SARS (MQR2.3901), determined by ELISA. Antibody stock 1.38 mg/ml)

diluted in PBS containing 0.05% tween-20 and 1% BSA was tested on human Seryl-tRNAsynthetase (in blue) and non-target protein (in red)