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现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips
现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips
  • 现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips

现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips

产品报价:询价

更新时间:2023/4/13 10:58:11

地:其他国家

牌:aimbiotech

号:AIM

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词: AIM 3D Cell Culture Chips   3D与2D细胞培养   3D versus 2D cell culture   aimbiotech   现货  

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Technology

3D versus 2D cell culture

In vitro 3D cell culture models have emerged as a bridge between traditional 2D cell culture models and in vivo animal models. A carefully designed 3D model provides more physiologically relevant data for cell proliferation, morphology, dose response to drugs/toxins and gene expression profiles. This is because biological mechanisms that occur in 3D differ significantly from 2D culture in ways more consistent with in vivo observations [1-3]. The topographically complex 3D microenvironment accurately reflects in vivo conditions than a hard & flat plastic surface. Cell adhesion, cellular structure, effector transport and mechanotransduction are also substantially different in 3D systems [4]. This enables users to design experiments that previously could not be performed in conventional 2D assays, at a fraction of the cost of in vivo animal models. 
  1. Pampaloni, F., E.G. Reynaud, and E.H.K. Stelzer, The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol, 2007. 8(10): p. 839-845.

  2. Antoni, D., et al., Three-Dimensional Cell Culture: A Breakthrough in Vivo. International Journal of Molecular Sciences, 2015. 16(3): p. 5517.

  3. Ravi, M., et al., 3D Cell Culture Systems: Advantages and Applications. Journal of Cellular Physiology, 2015. 230(1): p. 16-26.

  4. Baker, B.M. and C.S. Chen, Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues. Journal of Cell Science, 2012. 125(13): p. 3015-3024.

PUBLICATIONS
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Confocal image of angiogenic sprouts

Microfluidic devices for cell culture

Using microfluidic technologies for 3D cell culture brings additional benefits:
  • Microfluidic devices require small volumes of culture media and small quantities of cells, leading to reduced running costs. Studies can be conducted in cases where the cell source is limited (e.g. clinical samples) 

  • Microfluidic devices have low space requirements given their small footprints, making it possible to scale up experimental throughput

  • Compartmentalisation of cells into different channels/zones & live cell imaging analysis enable experimental designs with spatiotemporal elements

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The underside of a microfluidic chip showing the size of its channels (250 microns deep). The chip is 25mm wide, measured from the edges shown above.

Key features of AIM microfluidic chips

  • Long working regions that are easily injectable with hydrogel, with low risk of leakage

  • Gas permeable bottom laminate ensures accurate reflections of incubator conditions (normoxic or hypoxic)

  • Multicellular co-culture, with meaningful organization into models of biological systems

  • Control over chemical gradients and flow across the gel region and/or within the media channels



Novel posts enable easy gel filling & long working regions

AIM 3D Cell Culture Chips utilize a patented approach with a novel post design in conjunction with optimized post spacing & channel height. This allows hydrogels to be contained within gel channels-during the hydrogel filling process, with little risk of leaking into adjacent channels. The DAX-1 chip, for instance, has a 10.5mm long gel region. 
The air-liquid interface is substantially flat & uniform, with minimal occurrence of concave (under-filled) or convex (over-filled) interfaces. As the hydrogel is caged within the gel channel, the meniscus that usually obstructs phase contrast imaging is also absent. In short, AIM chips make it easy for users to cast hydrogels for 3D cell culture, and provide excellent optical clarity for various imaging techniques.
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Gas permeable laminate

AIM 3D Cell Culture Chips are fabricated with gas permeable laminates to ensure that oxygen tension in each chip correctly reflects incubator conditions. Users have the flexibility of setting up normoxic or hypoxic culture conditions.
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Multicellular culture made possible, with meaningful organization into models of biological systems

The multi-channel design of AIM 3D Cell Culture Chips enables the co-culture of different cell types in distinct compartments in the device, yet allowing paracrine signalling between cell types to take place. The movement of cells between different channels (or within an individual channel) can be easily observed & tracked. 
The growth and/or migration of cells within gel can often cause gel shrinkage or degradation. This problem is mitigated by the use of posts in AIM chips. The posts help to stabilize the gel and increase cell culture duration before the matrix collapses.

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Control over chemical gradients & interstitial flow

A chemical concentration gradient can easily be created across the porous 3D hydrogel by using a higher concentration of the chemical in a channel and allowing diffusion to take place.  This feature is very useful for studies where directional cues of effectors are critical, including angiogenesis, cell migration and neurite guidance. 
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Illustration of a chemical gradient
The interstitial flow across the 3D hydrogel can be controlled by setting up a pressure gradient between the flanking channels. This can be achieved by having a larger media volume in one media channel than the other, or by setting shear flow regimes that establish a pressure differential.  
AIM chips enable users to control shear flow in media channels with/without creating a pressure gradient across the gel channel.  Shear flows are typically set by connecting the chip to a standard syringe pump through accessory connectors.
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Interstitial and shear flows

Selected Publications

  • Vickerman V, Blundo J, Chung S and Kamm RD. Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging  Lab Chip, 2008, 8, 1468–1477,   DOI: 10.1039/b802395f

  • Farahat W, Wood L, Zervantonakis I, Schor A, Ong S, Neal D, Kamm RD, and Asada H. Ensemble Analysis of Angiogenic Growth in Three-Dimensional Microfluidic Cell Cultures  PLoS ONE 7(5): e37333. doi:10.1371/journal.pone.0037333, May 2012.

  • Shin Y, Han S, Jeon JS, Yamamoto K, Zervantonakis IK, Sudo R, Kamm RD and Chung S. Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels.  Nature Prot, 7(7):1247-1259, 2012, PMID: 22678430

现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips现货,3D versus 2D cell culture,3D与2D细胞培养,AIM 3D Cell Culture Chips