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现在位置首页>实验室原料试剂耗材>生化试剂>细胞生物学用试剂>现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶
现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶
现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶
  • 现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶

现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:Flexcell

号:Thermacol

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词: 三维细胞水凝胶   Thermacol   3D细胞培养水凝胶   现货   kit  

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现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶现货,Thermacol Kit 三维细胞水凝胶,3D细胞培养水凝胶

Flexcell? Thermacol? Kit


Collagen gel solution with rapid gelation at 37 C (see Fig. 1 below).
  • All components in one kit for creating a 3D cell-seeded bioartificial type I collagen gel (see Fig. 2 below).

  • Multiple applications including tissue engineering, migration studies, differentiation, chemotaxis, cell interactions, & matrix interactions.

  • Kit contains atelopeptide and telopetide-containing type I collagen, 5X MEM, fetal bovine serum, 1 M hepes, and 0.1 M NaOH.

  • Available in three sizes: mini, midi*, and maxi*.

*10% Savings (based on price of purchasing same number of mini kits individually)

Learn about Collagel?, another type I collagen hydrogel kit, here.


Applications of Thermacol?
  • 3D Cell Culture - culture cells in Collagel? to create a 3D cell-seeded type I collagen hydrogel.

  • Tissue Engineering - use Collagel? with a Tissue Train? culture plate to create bioartificial tissues.

  • Mechanical Load - culture cells in 3D then apply mechanical load with the Flexcell?Tension or Compression System.

  • Drug Discovery - culturing cells in a 3D environment better mimics in vivo conditions and thus provides a platform to study the effects of various pharmacological agents on cells in a more natural environment.

Read more about Tissue Engineering with a Flexcell? Culture System


Thermacol

Figure 1: Speed of gelation of 3D cell-seeded constructs made with Collagel? and Thermacol?.
Tissue Train

Figure 2: Bioartificial tissue development with Thermacol? and the Tissue Train? Culture System.

Relevant Tech Reports & Other Information
  • 100: Tissue Train? Culture System. A Method for Culture and Mechanical Loading of Cells in a Linear 3D Matrix

  • 104: Trapezoidal Trough Loader?. A Device for Fabricating 3-Dimensional Bioartificial Tissue Constructs

  • 107: ScanFlex? with XyFlex?. An Automated Method to Measure Gel Compaction in Three Dimensional Bioartificial Tissues

  • 113: Tissue Train? Anchor Options. Comparison between the non-woven nylon and the urethane polyester foam anchors.

  • 114: Collagel? and ThermaCol?. Collagen I Hydrogel Kits for 3D Cell Culture.

  • 207: Methods for Immunochemical Staining of Cells in Three Dimensional Bioartificial Tissues (BATS) 

  • Collagel? Product Information Sheet

  • Thermacol? Product Information Sheet

  • Circular Foam Tissue Train? Culture Plates Product Information

  • Linear Tissue Train? Culture Plates Product Information

  • Trapezoidal Tissue Train? Culture Plates Product Information

  • Flexcell? Transwell Holder Product Information 

  • Flexcell? Tissue Train? Culture System User Manual


Recent Publications with a Flexcell? Tissue Engineering Product

Applied stretch initiates directional invasion through the action of Rap1 GTPase as a tension sensor
Freeman SA, Christian S, Austin P, Iu I, Graves ML, Huang L, Tang S, Coombs D, Gold MR, Roskelley CD. J Cell Sci 130(1):152-163, 2017. doi: 10.1242/jcs.180612.

The myocardial regenerative potential of three-dimensional engineered cardiac tissues composed of multiple human iPS cell-derived cardiovascular cell lineages
Masumoto H, Nakane T, Tinney JP, Yuan F, Ye F, Kowalski WJ, Minakata K, Sakata R, Yamashita JK, Keller BB. Sci Rep 6:29933, 2016. doi: 10.1038/srep29933.

Duration and magnitude of myofascial release in 3-dimensional bioengineered tendons: effects on wound healing
Cao TV, Hicks MR, Zein-Hammoud M, Standley PR. Osteopath Assoc 115(2):72-82, 2015. doi: 10.7556/jaoa.2015.018.

Effects of physiologic mechanical stimulation on embryonic chick cardiomyocytes using a microfluidic cardiac cell culture model
Nguyen MD, Tinney JP, Ye F, Elnakib AA, Yuan F, El-Baz A, Sethu P, Keller BB, Giridharan GA. Anal Chem 87(4):2107-13, 2015. doi: 10.1021/ac503716z. Epub 2015 Feb 2.

Mechanical stress promotes maturation of human myocardium from pluripotent stem cell-derived progenitors
Ruan JL, Tulloch NL, Saiget M, Paige SL, Razumova MV, Regnier M, Tung KC, Keller G, Pabon L, Reinecke H, Murry CE. Stem Cells 33(7):2148-57, 2015. doi: 10.1002/stem.2036. Epub 2015 May 11.

Effects of intermittent and incremental cyclic stretch on ERK signaling and collagen production in engineered tissue
Schmidt JB, Chen K, Tranquillo RT. Cellular and Molecular Bioengineering 1-10, 2015. doi:10.1007/s12195-015-0415-6.

Mechanical stretch assays in cell culture systems
Tondon A, Haase C, Kaunas R. In: Handbook of Imaging in Biological Mechanics, ed. Neu CP, Genin GM. CRC Press: Boca Raton, 2015.

Degree of scaffold degradation influences collagen (re)orientation in engineered tissues
de Jonge N, Foolen J, Brugmans MC, S?ntjens SH, Baaijens FP, Bouten CV. Tissue Eng Part A 20(11-12):1747-57, 2014. doi: 10.1089/ten.TEA.2013.0517. 

Cyclic mechanical strain induces TGFβ1-signalling in dermal fibroblasts embedded in a 3D collagen lattice
Peters AS, Brunner G, Krieg T, Eckes B. Arch Dermatol Res 2014 Oct 28.

Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells
Tchao J, Han L, Lin B, Yang L, Tobita K. Sci Rep 4:6614, 2014. doi: 10.1038/srep06614.

Application of polarization-sensitive OCT and Doppler OCT in tissue engineering
Yang Y, Wimpenny I, Wang RK. In: Optical Techniques in Regnerative Medicine, edited by Morgan SP, Rose F, Matcher SJ. Taylor & Francis Group: Florida, p. 307-327, 2014.

Matrix rigidity activates Wnt signaling through down-regulation of Dickkopf-1 protein
Barbolina MV, Liu Y, Gurler H, Kim M, Kajdacsy-Balla AA, Rooper L, Shepard J, Weiss M, Shea LD, Penzes P, Ravosa MJ, Stack MS. J Biol Chem 288(1):141-51, 2013. doi: 10.1074/jbc.M112.431411. 

Dosed myofascial release in three-dimensional bioengineered tendons: effects on human fibroblast hyperplasia, hypertrophy, and cytokine secretion
Cao TV, Hicks MR, Campbell D, Standley PR. J Manipulative Physiol Ther 36(8):513-21, 2013. doi: 10.1016/j.jmpt.2013.07.004. 

Ablation of cardiac myosin-binding protein-C accelerates contractile kinetics in engineered cardiac tissue
de Lange WJ, Grimes AC, Hegge LF, Ralphe JC. J Gen Physiol 141(1):73-84, 2013. doi: 10.1085/jgp.201210837.

Cyclical strain modulates metalloprotease and matrix gene expression in human tenocytes via activation of TGFβ
Jones ER, Jones GC, Legerlotz K, Riley GP. Biochim Biophys Acta 1833(12):2596-2607, 2013. doi: 10.1016/j.bbamcr.2013.06.019.

Engineered human muscle tissue from skeletal muscle derived stem cells and induced pluripotent stem cell derived cardiac cells
Tchao J, Kim JJ, Lin B, Salama G, Lo CW, Yang L, Tobita K. International Journal of Tissue Engineering 2013 Article ID 198762, 15 pages, 2013. http://dx.doi.org/10.1155/2013/198762.

Combating adaptation to cyclic stretching by prolonging activation of extracellular signal-regulated kinase
Weinbaum JS, Schmidt JB, Tranquillo RT. Cellular and Molecular Bioengineering 6 (3):279-286, 2013.

Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix
Yang G, Rothrauff BB, Lin H, Gottardi R, Alexander PG, Tuan RS. Biomaterials 34(37):9295-306, 2013. doi: 10.1016/j.biomaterials.2013.08.054.

Gene expression profiles in engineered cardiac tissues respond to mechanical loading and inhibition of tyrosine kinases
Ye F, Yuan F, Li X, Cooper N, Tinney JP, Keller BB. Physiol Rep 1(5):e00078, 2013. doi: 10.1002/phy2.78.


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