KET7001 *EliKine™ Mouse CCL2 ELISA Kit 小鼠趋化因子CCL2 ELISA定量试剂盒 北京现货

Product name：*EliKine? Mouse CCL2 ELISA Kit 小鼠趋化因子CCL2 ELISA定量试剂盒 北京现货

Reactivity：Mouse

Applications：ELISA

Application notes：

EliKine? Mouse CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL2 in samples. An antibody specific for CCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL2 is added to the wells. After washing, proprietary EliKine? Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Assay duration：

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

*EliKine? Mouse CCL2 ELISA Kit 小鼠趋化因子CCL2 ELISA定量试剂盒 北京现货

Kit components：

 Mouse CCL2 microplate
 Mouse CCL2 standard
 Mouse CCL2 detect antibody
 EliKine? Streptavidin-HRP
 Standard diluent
 Assay buffer
 HRP substrate
 Stop solution
 Wash buffer
 Plate covers.

Features & Benefits：

*EliKine? Mouse CCL2 ELISA Kit 小鼠趋化因子CCL2 ELISA定量试剂盒 北京现货 has high sensitivity and excellent specificity for detection of Mouse CCL2. No significant cross-reactivity or interference between Mouse CCL2 and analogues was observed.

Usage notes：

? Do not mix components from different kit lots or use reagents beyond the kit expiration date.
? Allow all reagents to warm to room temperature for at least 30 minutes before opening.
? Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid c   ontamination.
? Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
? Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight   hand shaking every 10 minutes.
? It is recommended that all samples and standards be assayed in duplicate or triplicate.

*EliKine? Mouse CCL2 ELISA Kit 小鼠趋化因子CCL2 ELISA定量试剂盒 北京现货

Storage instructions：

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Precautions：

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background：

CCL2 is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). This chemokine is produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa.CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. Expression of this proinflammatory chemokine is increased in atherosclerotic lesions, and inhibition of it expression or that of its receptor, CC chemokine receptor 2 (CCR2), reduces the extent of atheroma formation in hypercholesterolemic mice.