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高通量单细胞机械力特性微流控测试分析系统,单细胞微流体形变仪系统,单细胞微流控形变仪系统
高通量单细胞机械力特性微流控测试分析系统,单细胞微流体形变仪系统,单细胞微流控形变仪系统
  • 高通量单细胞机械力特性微流控测试分析系统,单细胞微流体形变仪系统,单细胞微流控形变仪系统

高通量单细胞机械力特性微流控测试分析系统,单细胞微流体形变仪系统,单细胞微流控形变仪系统

产品报价:询价

更新时间:2020/2/27 10:40:39

地:德国

牌:rszelltechnik

号:optical strecher

厂商性质: 生产型,贸易型,服务型,

公司名称: 世联博研(北京)科技有限公司

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单细胞牵张拉伸压缩形变与机械力特性测试分析系统

——基于微流控技术的单细胞机械力特性精准、高通量表征
Single cell mechanics - the easy way

背景:
单细胞水平的机械力特性表征,可以有效阐明细胞的功能和状态,揭示细胞的单体差异性,对于细胞的分化和病理研究,以及疾病的早期临床诊断和治疗具有非常重要的意义。 该系统基于微流控芯片的方式更适合单细胞样本的微环境精确控制、高通量定向操纵及多参数非特异性检测。
简介:
该单细胞高通量牵张与力学特性测试分析系统,是世界第一台用来高通量测量、分析单个悬浮细胞形变的设备。用来可视化研究讨论细胞力学性质与其功能之间的关系 该系统可以安装在任何相位差显微镜上的模块。温度稳定和激光安全。
单细胞牵张拉伸压缩形变与机械力特性测试分析系统  

单个细胞加力模式如下图:
由两束细胞无损激光夹持细胞进行牵拉或挤压,进而使单个细胞受到牵张拉伸或压缩力刺激。



系统亮点特性:

1 )可大量表征单细胞机械力特性、操作简便、样品消耗量小
该系统的微流控芯片具有与细胞直径良好相符性的微纳米级腔道,并能实现对微流体的精确控制,使其尤其适合单细胞机械特性研究分析,该微流控的高通量技术便于大量表征单细胞机械力特性、操作简便、样品消耗量小、集成和微型化程度高等优点,且在分析过程中单细胞悬浮高速流经检测区域,该连续流动态检测的特性极大提高了系统的通量。
2)高速对单个细胞进行形变,并进行机械特性高速表征,单细胞高通量流变
利用两素未聚焦光进行单细胞形变,并通过图形化微柱基地表征细胞的力特性,高速有效分析单细胞水平的机械特性, 高达300个细胞/小时.
3)非机械接触、无标记进行细胞捕捉和拉伸,确保细胞安全与细胞损伤最小化(Contact-free cell deformation)
利用光延伸器技术测试细胞机械特性能时,在非机械接触情况下细胞进行捕捉和拉伸,且不需要对激光进行聚焦,能实现细胞损伤最小化。优于AFM(原子力显微镜)和光镊
4)将光延伸器安全性与微流控高通量完美相结合,细胞机械特性测试分析安全而且高效
采用2个微流道来输送细胞,使两条光纤垂直分布于通道两侧并严格对准? ,单细胞随流体进入检测区域时,首先采用功率较低的光速捕获细胞,然后增加光速的功率使细胞发生形变。通过对细胞变形能力的分析,不仅能区分病变细胞和正常细胞,而且可以用于辨别转型特性和非转移特性的癌细胞。
5)自动化测量单细胞力属性和成像记录细胞形变记录
对应于用户定义的拉伸模式,细胞被自动传送到测量区域由CellStretcher模块控制所有组件和自动测量细胞;细胞形变由系统CCD相机自动记录,并由CellEvaluator自动提取记录显微图像形变数据,CellReporter可视化统计分析表征参数。在光学拉伸加载运行实验中,科研学者可专注于阐述实验结果
6)良好温控微环境罩



单细胞牵张拉伸压缩形变与机械力特性测试分析系统


单细胞牵张拉伸压缩形变与机械力特性测试分析系统

单细胞牵张拉伸压缩形变与机械力特性测试分析系统

Publications

RS ZELLTECHNIK BROCHURES

The Optical Stretcher

OPTICAL STRETCHER TECHNOLOGY

Lincoln, B., Schinkinger, S., Travis, K., Wottawah, F., Ebert, S., Sauer, F., Guck, J., 2007. Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications. Biomed. Microdevices 9, 703–710. doi:10.1007/s10544-007-9079-x
Ebert, S., Travis, K., Lincoln, B., Guck, J., 2007. Fluorescence ratio thermometry in a microfluidic dual-beam laser trap. Opt. Express 15, 15493–15499. doi:10.1364/OE.15.015493
Jensen-McMullin, C., Lee, H.P., Lyons, E.R.L., 2005. Demonstration of trapping, motion control, sensing and fluorescence detection of polystyrene beads in a multi-fiber optical trap. Opt. Express 13, 2634–2642. doi:10.1364/OPEX.13.002634
Wottawah, F., Schinkinger, S., Lincoln, B., Ananthakrishnan, R., Romeyke, M., Guck, J., K?s, J., 2005. Optical Rheology of Biological Cells. Phys. Rev. Lett. 94, 098103. doi:10.1103/PhysRevLett.94.098103
Lincoln, B., Erickson, H.M., Schinkinger, S., Wottawah, F., Mitchell, D., Ulvick, S., Bilby, C., Guck, J., 2004. Deformability-based flow cytometry.Cytometry A 59A, 203–209. doi:10.1002/cyto.a.20050

THEORETICAL MODELS

Ananthakrishnan, R., Guck, J., Wottawah, F., Schinkinger, S., Lincoln, B., Romeyke, M., Kas, J., 2005. Modelling the structural response of an eukaryotic cell in the optical stretcher. Curr. Sci. 88.
B. Bareil, P., Sheng, Y., Chiou, A., 2006. Local scattering stress distribution on surface of a spherical cell in optical stretcher. Opt. Express 14, 12503–12509. doi:10.1364/OE.14.012503
Bareil, P.B., Sheng, Y., Chen, Y.-Q., Chiou, A., 2007. Calculation of spherical red blood cell deformation in a dual-beam optical stretcher. Opt. Express 15, 16029–16034. doi:10.1364/OE.15.016029
Boyde, L., Ekpenyong, A., Whyte, G., Guck, J., 2012. Comparison of stresses on homogeneous spheroids in the optical stretcher computed with geometrical optics and generalized Lorenz–Mie theory. Appl. Opt. 51, 7934–7944. doi:10.1364/AO.51.007934
Ekpenyong, A.E., Posey, C.L., Chaput, J.L., Burkart, A.K., Marquardt, M.M., Smith, T.J., Nichols, M.G., 2009. Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher. Appl. Opt. 48, 6344–6354. doi:10.1364/AO.48.006344
Teo, S.-K., Goryachev, A.B., Parker, K.H., Chiam, K.-H., 2010. Cellular deformation and intracellular stress propagation during optical stretching.Phys. Rev. E 81, 051924. doi:10.1103/PhysRevE.81.051924

CANCER RESEARCH AND DIAGNOSTICS

Kastl, L., Budde, B., Isbach, M., Rommel, C., Kemper, B., Schnekenburger, J., 2015. Optomechanical properties of cancer cells revealed by light-induced deformation and quantitative phase microscopy. pp. 952908–952908–6. doi:10.1117/12.2184764
Martin, M., Müller, K., Cadenas, C., Hermes, M., Zink, M., Hengstler, J.G., K?s, J.A., 2012. ERBB2 overexpression triggers transient high mechanoactivity of breast tumor cells. Cytoskeleton 69, 267–277. doi:10.1002/cm.21023
Fritsch, A., H?ckel, M., Kiessling, T., Nnetu, K.D., Wetzel, F., Zink, M., K?s, J.A., 2010. Are biomechanical changes necessary for tumour progression? Nat. Phys. 6, 730–732. doi:10.1038/nphys1800
Brunner, C., Niendorf, A., K?s, J.A., 2009. Passive and active single-cell biomechanics: a new perspective in cancer diagnosis. Soft Matter 5, 2171–2178. doi:10.1039/B807545J
Remmerbach, T.W., Wottawah, F., Dietrich, J., Lincoln, B., Wittekind, C., Guck, J., 2009. Oral Cancer Diagnosis by Mechanical Phenotyping. Cancer Res. 69, 1728–1732. doi:10.1158/0008-5472.CAN-08-4073
Martin, M., Mueller, K., Wottawah, F., Schinkinger, S., Lincoln, B., Romeyke, M., K?s, J.A., 2006. Feeling with light for cancer. p. 60800P–60800P–10. doi:10.1117/12.637899
Guck, J., Schinkinger, S., Lincoln, B., Wottawah, F., Ebert, S., Romeyke, M., Lenz, D., Erickson, H.M., Ananthakrishnan, R., Mitchell, D., K?s, J., Ulvick, S., Bilby, C., 2005. Optical Deformability as an Inherent Cell Marker for Testing Malignant Transformation and Metastatic Competence. Biophys. J. 88, 3689–3698. doi:10.1529/biophysj.104.045476

STEM CELL RESEARCH

Ekpenyong, A.E., Whyte, G., Chalut, K., Pagliara, S., Lautenschlaeger, F., Fiddler, C., Paschke, S., Keyser, U.F., Chilvers, E.R., Guck, J., 2012.Viscoelastic Properties of Differentiating Blood Cells Are Fate- and Function-Dependent. Plos One 7, e45237. doi:10.1371/journal.pone.0045237
Galle, J., Bader, A., Hepp, P., Grill, W., Fuchs, B., Kas, J.A., Krinner, A., MarquaB, B., Muller, K., Schiller, J., Schulz, R.M., von Buttlar, M., von der Burg, E., Zscharnack, M., Loffler, M., 2010. Mesenchymal Stem Cells in Cartilage Repair: State of the Art and Methods to monitor Cell Growth, Differentiation and Cartilage Regeneration. Curr. Med. Chem. 17, 2274–2291. doi:10.2174/092986710791331095
Maloney, J.M., Nikova, D., Lautenschlager, F., Clarke, E., Langer, R., Guck, J., Van Vliet, K.J., 2010. Mesenchymal Stem Cell Mechanics from the Attached to the Suspended State. Biophys. J. 99, 2479–2487. doi:10.1016/j.bpj.2010.08.052
Lautenschl?ger, F., Paschke, S., Schinkinger, S., Bruel, A., Beil, M., Guck, J., 2009. The regulatory role of cell mechanics for migration of differentiating myeloid cells. Proc. Natl. Acad. Sci. 106, 15696–15701 doi:10.1073/pnas.0811261106

IMMUNE SYSTEM

Man, S.M., Ekpenyong, A., Tourlomousis, P., Achouri, S., Cammarota, E., Hughes, K., Rizzo, A., Ng, G., Wright, J.A., Cicuta, P., Guck, J.R., Bryant, C.E., 2014. Actin polymerization as a key innate immune effector mechanism to control Salmonella infection. Proc. Natl. Acad. Sci. 201419925 doi:10.1073/pnas.1419925111

BASIC RESEARCH

Schmidt, B.U.S., Kie?ling, T.R., Warmt, E., Fritsch, A.W., Stange, R., K?s, J.A., 2015. Complex thermorheology of living cells. New J. Phys. 17, 073010. doi:10.1088/1367-2630/17/7/073010
Chan, C.J., Ekpenyong, A.E., Golfier, S., Li, W., Chalut, K.J., Otto, O., Elgeti, J., Guck, J., Lautenschl?ger, F., 2015. Myosin II Activity Softens Cells in Suspension. Biophys. J. 108, 1856–1869. doi:10.1016/j.bpj.2015.03.009
Gladilin, E., Gonzalez, P., Eils, R., 2014. Dissecting the contribution of actin and vimentin intermediate filaments to mechanical phenotype of suspended cells using high-throughput deformability measurements and computational modeling. J. Biomech. 47, 2598–2605. doi:10.1016/j.jbiomech.2014.05.020
Maloney, J.M., Vliet, K.J.V., 2014. Chemoenvironmental modulators of fluidity in the suspended biological cell. Soft Matter. doi:10.1039/C4SM00743C
Warmt, E., Kie?ling, T.R., Stange, R., Fritsch, A.W., Zink, M., K?s, J.A., 2014. Thermal instability of cell nuclei. New J. Phys. 16, 073009. doi:10.1088/1367-2630/16/7/073009
Gyger, M., Stange, R., Kiessling, T.R., Fritsch, A., Kostelnik, K.B., Beck-Sickinger, A.G., Zink, M., Kaes, J.A., 2014. Active contractions in single suspended epithelial cells. Eur. Biophys. J. Biophys. Lett. 43, 11–23. doi:10.1007/s00249-013-0935-8
Seltmann, K., Fritsch, A.W., K?s, J.A., Magin, T.M., 2013. Keratins significantly contribute to cell stiffness and impact invasive behavior. Proc. Natl. Acad. Sci. 201310493. doi:10.1073/pnas.1310493110
Maloney, J.M., Lehnhardt, E., Long, A.F., Van Vliet, K.J., 2013. Mechanical fluidity of fully suspended biological cells. Biophys. J. 105, 1767–1777. doi:10.1016/j.bpj.2013.08.040
Kie?ling, T.R., Stange, R., K?s, J.A., Fritsch, A.W., 2013. Thermorheology of living cells—impact of temperature variations on cell mechanics. New J. Phys. 15, 045026. doi:10.1088/1367-2630/15/4/045026
Kie?ling, T.R., Herrera, M., Nnetu, K.D., Balzer, E.M., Girvan, M., Fritsch, A.W., Martin, S.S., K?s, J.A., Losert, W., 2013. Analysis of multiple physical parameters for mechanical phenotyping of living cells. Eur. Biophys. J. 42, 383–394. doi:10.1007/s00249-013-0888-y
Paschke, S., Weidner, A.F., Paust, T., Marti, O., Beil, M., Ben-Chetrit, E., 2013. Technical advance: Inhibition of neutrophil chemotaxis by colchicine is modulated through viscoelastic properties of subcellular compartments. J. Leukoc. Biol. 94, 1091–1096. doi:10.1189/jlb.1012510
Chalut, K.J., H?pfler, M., Lautenschl?ger, F., Boyde, L., Chan, C.J., Ekpenyong, A., Martinez-Arias, A., Guck, J., 2012. Chromatin decondensation and nuclear softening accompany Nanog downregulation in embryonic stem cells. Biophys. J. 103, 2060–2070. doi:10.1016/j.bpj.2012.10.015
Matthews, H.K., Delabre, U., Rohn, J.L., Guck, J., Kunda, P., Baum, B., 2012. Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression. Dev. Cell 23, 371–383. doi:10.1016/j.devcel.2012.06.003
Mauritz, J.M.A., Esposito, A., Tiffert, T., Skepper, J.N., Warley, A., Yoon, Y.-Z., Cicuta, P., Lew, V.L., Guck, J.R., Kaminski, C.F., 2010. Biophotonic techniques for the study of malaria-infected red blood cells. Med. Biol. Eng. Comput. 48, 1055–1063. doi:10.1007/s11517-010-0668-0
Rusciano, G., 2010. Experimental analysis of Hb oxy–deoxy transition in single optically stretched red blood cells. Phys. Med. 26, 233–239. doi:10.1016/j.ejmp.2010.02.001

AGING PROCESSES

Schulze, C., Wetzel, F., Kueper, T., Malsen, A., Muhr, G., Jaspers, S., Blatt, T., Wittern, K.-P., Wenck, H., K?s, J.A., 2010. Stiffening of Human Skin Fibroblasts with Age. Biophys. J. 99, 2434–2442. doi:10.1016/j.bpj.2010.08.026

VESICLES

Delabre, U., Feld, K., Crespo, E., Whyte, G., Sykes, C., Seifert, U., Guck, J., 2015. Deformation of phospholipid vesicles in an optical stretcher. Soft Matter. doi:10.1039/C5SM00562K
Solmaz, M.E., Sankhagowit, S., Biswas, R., Mejia, C.A., Povinelli, M.L., Malmstadt, N., 2013. Optical stretching as a tool to investigate the mechanical properties of lipid bilayers. Rsc Adv. 3, 16632–16638. doi:10.1039/c3ra42510j
Solmaz, M.E., Biswas, R., Sankhagowit, S., Thompson, J.R., Mejia, C.A., Malmstadt, N., Povinelli, M.L., 2012. Optical stretching of giant unilamellar vesicles with an integrated dual-beam optical trap. Biomed. Opt. Express 3, 2419–2427. doi:10.1364/BOE.3.002419

TECHNICAL ADVANCES

Grosser, S., Fritsch, A.W., Kie?ling, T.R., Stange, R., K?s, J.A., 2015. The lensing effect of trapped particles in a dual-beam optical trap. Opt. Express 23, 5221–5235. doi:10.1364/OE.23.005221
Bellini, N., Bragheri, F., Cristiani, I., Guck, J., Osellame, R., Whyte, G., 2012. Validation and perspectives of a femtosecond laser fabricated monolithic optical stretcher. Biomed. Opt. Express 3, 2658–2668. doi:10.1364/BOE.3.002658
Bellini, N., Vishnubhatla, K.C., Bragheri, F., Ferrara, L., Minzioni, P., Ramponi, R., Cristiani, I., Osellame, R., 2010. Femtosecond laser fabricated monolithic chip for optical trapping and stretching of single cells. Opt. Express 18, 4679–4688. doi:10.1364/OE.18.004679