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现在位置首页>实验试剂制品>核酸分析>核酸修饰>供应美国Expression Systems DNA及载体分子产品 Molecular Products DNA and Vectors
供应美国Expression Systems DNA及载体分子产品 Molecular Products DNA and Vectors
供应美国Expression Systems DNA及载体分子产品 Molecular Products DNA and Vectors
  • 供应美国Expression Systems DNA及载体分子产品 Molecular Products DNA and Vectors

供应美国Expression Systems DNA及载体分子产品 Molecular Products DNA and Vectors

产品报价:询价

更新时间:2019/8/22 13:54:40

地:美国

牌:Expression Systems

号: 91-200

厂商性质: 生产型,贸易型,服务型,

公司名称: 重庆渝时兴科技有限公司

产品关键词:

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BestBac 2.0, v-cath/chiA Deleted Baculovirus Cotransfection Kit

  • 91-200
  • Molecular Products DNA and Vectors
  • The BestBac 2.0, v-cath/chiA Deleted Baculovirus Cotransfection Kit Transfection kit contains everything you need to perform co-transfections with your transfer vector. Included in the kit are:

    BestBac 2.0 v-cath/chiA deleted DNA  2.5 ug
    pVL1393-lacZ Control Vector  5 ug     
    Transfection Medium 20 ml
    Expres2TR Transfection Reagent 0.75 ul

    The BestBac viral backbone is available in a form that is wild type at the cathepsin and chitinase locus and as a deletion mutant. The v-cath/chiA deletion removes a potentially detrimental protease and may reduce competition for resources for protein synthesis during late gene expression. The v-cath/chiA deletion can improve the integrity of your expressed protein resulting in increased functional protein yield.

    Expression Systems’ BestBac DNA provides a means for producing recombinant baculoviruses with efficiencies close to 100%. The principle of this technique lies in the production of baculovirus DNA which contains a deletion in an essential gene. Only co-transfection of insect cells with the viral DNA and a complementing transfer vector can reconstitute a viable virus.

    BestBac DNA is derived from the E2 clone of AcMNPV which has been alternately passaged in-vitro and in-vivo to ensure no loss of viral integrity.  The polyhedrin locus of BestBac has been modified to remove only the polyhedrin promoter and coding sequence therefore all polyhedrin gene locus-based baculovirus transfer vectors can be used to complement the BestBac deletion. Transfer vectors include pVL1392, pVL1393 and their derivatives.
  • 1.    Molecular Products DNA and Vectors   DNA及载体分子产品

Product #

Product

Code

Size

91-200

BestBac 2.0, v-cath/chiA Deleted Baculovirus  Cotransfection Kit

91-200

1 kit

91-002

BestBac 2.0, v-cath/chiA Deleted Linearized Baculovirus  DNA

91-002

2.5 ug

91-200

complete kit

91-030

pVL1392, 1393 Transfer Vector Set

91-030

1 set

95-020

Transfection Medium

95-020-20

20mL Bottle

95-020-50

50mL Bottle

95-020-100

100mL Bottle

91-001

BestBac 1.0, Linearized Baculovirus DNA

91-001

2.5 ug

91-100

BestBac 1.0, Baculovirus Cotransfection Kit

91-100

complete kit

91-100

1 kit