Flocel体外三维动态血脑屏障系统
特点
1.2 配置及参数资料 TEER检测系统 CARTRIDGE底板 4个DIV-BBB 盒子 检测软件 电缆线 DIV-BBB?管腔 特性 | 优点 | 可控制腔内/非腔体积比 体型小,仅2.57cm长 电极与管腔一体化 成本低 | 与体内体积比相符 减少了试验所需细胞数目 TEER检测系统操作简单 一次性使用,不能重复使用 |
疏水性毛细血管(Hydrophobic capillaries) Accurel PP Q3/2 (optionally pre-coated with ProNectin For Poly-D-Lysine) 经毛细管孔径: nominal 0.2um 中空纤维数量:19 腔内总体积 : 0.0123in3 = 0.202cm3 腔内表面积 : 2.09 in3= 13.5cm2 腔外体积 :0.070 in3 = 1.15cm3 腔外中空纤维总面积: 3.50 in3 = 22.6cm2 跨细胞膜电阻测量(TEER 检测)系统 TEER检测系统为研发人员提供了一个快速、简便地评估血脑屏障完整性方法,此外,电阻抗值(>1000 -cm2)也非常接近体内数值。TEER检测系统同时配带了所需检测软件、电线和衬板,可供4个DIV-BBB培养管腔同时使用。其中检测软件可以自定义设置泵的流速、频率、波形等参数。其它特性及优点见下表: 特性 | 优点 | 多频率下检测电阻抗 | 描述电极的电阻和电容 | 低电压,Max=60毫伏 | 限制对屏障的潜在破坏作用 | 自动检测多个管腔 | 可同时连接4个盒子 | 检测软件可自定义设置泵的流速、频率、波形等参数 | 检测电阻抗,记录数据 | USB连接 | 台式或者便携式电脑均可连接 |
计算机控制的脉动泵 脉搏率和作用周期完全模拟体内血液动力学设计,贴近体内环境,试验准确,脉搏率可设置在30-120/分范围内。其特性和优点如下:
特性 | 优点 | 4个管腔的流速和泵的速度可分别控制, | 允许4个独立试验 | 外部24电压墙与电源连接 | 减少培养箱中高温 | 接受来自TEER检测系统的指定程序 | 不需要额外的USB接口 |
泵速: 30-120bpm 流速: 1-50ml/min 电源: TEER控制 系统组成部件:白色系统底托(White plastic tray) 4 个通道体外细胞培养系统(4 DIV-BBB cartridges)及配套硅胶管 跨上皮细胞电阻测量系统(TEER Measurement System )/泵控制电缆(Pump Control Cable) 4套125毫升培养基玻璃瓶(4 glass 125ml bottles)及4套瓶盖(4 bottle caps) 4套33毫米的储液杯及盖子 (4 33mm Reservoir Caps) 计算机控制的Quad程控脉冲式灌流泵(Flocel Qual Computer-controlled Perfusion System)及配套硅胶管 8个鲁尔锁栓 (8 male luer locks) 4个三通阀 (4 3-way stopcocks) USB接口线 (USB cable) 电源线 (Power supply cable) Flocel控制测量系统软件(Flocel System Installation Software) 用户使用手册 (User’s Manual)
应用文献
Publications
The role of shear stress in Blood-Brain Barrier endothelial physiology. Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D. A dynamic in vitro BBB model for the study of immune cell trafficking into the central nervous system. Cucullo L, Marchi N, Hossain M, Janigro D. Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow. Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N. Tobacco smoke: a critical etiological factor for vascular impairment at the blood-brain barrier. Hossain M, Sathe T, Fazio V, Mazzone P, Weksler B, Janigro D, Rapp E, Cucullo L. Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies. Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D. Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: a permeability study. Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L. Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic drugs. Cucullo L, Hossain M, Rapp E, Manders T, Marchi N, Janigro D. Drug delivery and in vitro models of the blood-brain barrier. Cucullo L, Aumayr B, Rapp E, Janigro D. Glycerophosphoinositol and dexamethasone improve transendothelial electrical resistance in an in vitro study of the blood-brain barrier. Cucullo L, Hallene K, Dini G, Dal Toso R, Janigro D. Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis. Marroni M, Kight KM, Hossain M, Cucullo L, Desai SY, Janigro D. A new dynamic in vitro model for the multidimensional study of astrocyte-endothelial cell interactions at the blood-brain barrier. Cucullo L, McAllister MS, Kight K, Krizanac-Bengez L, Marroni M, Mayberg MR, Stanness KA, Janigro D. Mechanisms of endothelial survival under shear stress. Desai SY, Marroni M, Cucullo L, Krizanac-Bengez L, Mayberg MR, Hossain MT, Grant GG, Janigro D.
PostersA Dynamic Humanized drug resistant In Vitro Blood-Brain Barrier Model to assess the permeability of relevant CNS drugs Development of a novel blood-brain barrier model by coupling Immortalized human brain endothelial cells with flow-based cell culture technologies Glioblastoma Cell Line Metabolism Under Dynamic and Static Conditions: Effects on tumor growth and BBB integrity Humanized in vitro blood-brain barrier models to screen for brain penetration of antiepileptic drugs Effect of Shear stress on BBB endothelial cells: A proteomic study by 2-dimensional protein electrophoresis
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