产品报价：询价
更新时间：2023/4/19 15:26:00
产地：德国
品牌：pharma-cocell
型号：RBT-24F
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| BBB Kit? ( RBT-24H ) / ( RBT-24F )*,体外血脑屏障模型套件 |
| 型号:RBT-24H,RBT-24F |
| 品牌:pharmacocell |
BBB Kit? ( RBT-24H ) / ( RBT-24F )* BBB Kit? (RBT-24) is a new in vitro model of the blood-brain barrier (BBB) made of primary cultures of rat (Wistar rat) brain capillary endothelial cells, brain pericytes and astrocytes. BBB Kit? (RBT-24) with a polyethylene terephthalate (PT) membrane is a ready-to-use concept for your evaluations of BBB-permeability of drugs and drug candidates. BBB Kit? (RBT-24H) is a 12-well format constructed in a 24-well Millicell? cell culture plate.( *On-Demand Product - RBT-24F ) BBB Kit? (RBT-24H) is constructed in 12 wells (B and C) with Millicell? Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell? plate. [ H = Half ]*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit? in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our calculation of apparent permeability is Papp.**Cells within BBB Kit? (RBT-24) are not clearly seen by microscopic observations. ***PT membrane hardly absorbs chemicals. We deliver the complete set of BBB Kit? (RBT-24H) in frozen packaged with dry ice. BBB Kit? can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit (PRBT-24H) within 1 month. BBB Kit ? (RBT-24H)Contents:BBB Kit ? (RBT-24H)Thawing - and Incubation - MediumMillicell? Hanging Cell Culture Insert 4(for Blank experiments)货号品牌规格价格PCC-RBT-24HBBB Kit ?(RBT-24H)12 insertsSet17100元 Ready-To-Use BBB Kit ?We deliver the complete set of BBB Kit ? in frozen packaged with dry ice. BBB Kit ? can be frozen as a whole and stored at -80℃. We guarantee to use BBB Kit ? (RBT-24H) within 1 month.4 days prior to your experiment, you just thaw (de-freeze) your BBB Kit ? (RBT-24H) stored at -80℃ in your deep-freezer.Amounts of solutions (to be added)Thawing-solmedium (1)medium (2) *Blood-side200 μL300 μL300 μLBrain-side1000 μL1200 μL1200 μL* Recipe: medium 10% PDS / DMEM F-12Dulbecco's Modified Eagle's Medium/ Nutrient Mixture F-12 Ham (DMEM F-12)Fetal Bovine Plasma Derived Serum (PDS)10% (v/v)Heparin100 μg/mLbasic fibroblast growth factor (bFGF)1.5 ng/mLInsulin-Transferrin-sodium Selenite (ITS)insulin 5 μg/mL, transferrin 5 μg/mL, sodium selenite 5 ng/mLHydrocortisone500 nMGentamicin50 μg/mL Trans Endothelial Electrical Resistance (TEER) of BBB Kit ? (RBT-24)TEER in BBB Kit? (RBT-24) reaches more than 150 Ω x cm2, and maintains a plateau up to 6 days. (Medium does not contain cAMP and its analogs.) TEER is measured at Day 3 after changingmedium. BBB Kit? (RBT-24) can be used from Day 4 to Day 6 after thawing.【Equipment】 Insert Membrane InfomationInsert of BBB Kit? (RBT-24H) (Polyethylene terephthalate membrance filter)Millicell? Hanging Cell Culture Insert (#PISP 12R 48)Technical data of the polyethylene terephthalate (PT )membrane Millicell? Insert (#PISP 12R 48)Diameter:6.5 mmGrowth area:0.33 cm2Membrane thickness:9 μmPore size:3.0 μmPore density:2 x 106 pores / cm2Tissue culture treated:yesOptical property:translucentCell visibility:poorVolume inside insert:0.2 mL - 0.3 mLVolume in plate:0.9 mL - 1.2 mLMillicell? is a Registered Trade Mark of Millipore Corporation. BBB Kit? ( RBT-24F ) BBB Kit? (RBT-24F) is constructed in 24 wells with Millicell? Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell? plate. [ F = Full ]*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit? in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our Calculation of apparent permeability is Papp.**Cells within BBB Kit? (RBT-24) are not clearly seen by microscopic observations. ***PT membrane hardly absorbs chemicals. We deliver the complete set of BBB Kit? (RBT-24F) in frozen packaged with dry ice. BBB Kit? can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit (RBT-24F) within 1 month. BBB Kit ? (RBT-24F)Contents:BBB Kit ? (RBT-24F)Thawing - and Incubation - medium货号品名格式规格价格PCC-RBT-24F(On-Demand Product)BBB Kit ?(RBT-24F)24 insertsSet31000元 |
