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现在位置首页>行业专用>生命科学仪器>生命科学仪器>体外血脑屏障渗透性研究套件PCC-MBT-24F,BBB Kit (RBT-24H)
体外血脑屏障渗透性研究套件PCC-MBT-24F,BBB Kit (RBT-24H)
体外血脑屏障渗透性研究套件PCC-MBT-24F,BBB Kit (RBT-24H)
  • 体外血脑屏障渗透性研究套件PCC-MBT-24F,BBB Kit (RBT-24H)

体外血脑屏障渗透性研究套件PCC-MBT-24F,BBB Kit (RBT-24H)

产品报价:询价

更新时间:2019/4/2 12:09:54

地:德国

牌:pharma-cocell

号: PCC-MBT-24F

厂商性质: 生产型,贸易型,服务型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

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MBT-24H, MBT-24F由小分子到细胞药品血脑屏障渗透性研究套件,美国flocel 体外血脑屏障动力学实验仪器设备装置
型号:MBT-24H
价格:请致电:010-67529703
品牌:日本pharmacocell和美国flocel


BBB Kit? ( MBT-24H ) / ( MBT-24F )*
For Drug BBB Permeability Study ( from micromolecules to cells )



BBB Kit? (MBT-24) is a new in vitro model of the blood-brain barrier (BBB) made of primary cultures ofmonkey (Macaca irus) brain capillary endothelial cells, brain pericytes and astrocytes. BBB Kit? (MBT-24) with a polyethylene terephthalate (PT) membrane is a ready-to-use concept for your evaluations of BBB-permeability of drugs and drug candidates.
BBB Kit? (MBT-24H) is a 12-well format constructed in a 24-well Millicell? cell culture plate.
( *On-Demand Product - MBT-24F )

*Brain capillary endothelial cells are isolated from monkey (Macaca irus) brain, and brain pericytes and astrocytes are from rat (Wistar rat) brain.
BBB Kit? (MBT-24H) is constructed in 12 wells (B and C) with Millicell? Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell? plate. [ H = Half ]
*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit? in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our calculation of apparent permeability is Papp.
**Cells within BBB Kit? (MBT-24) are not clearly seen by microscopic observations.
***PT membrane hardly absorbs chemicals.

We deliver the complete set of BBB Kit? (MBT-24H) in frozen packaged with dry ice.
BBB Kit? can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit (MBT-24H) within 1 month.

BBB Kit ? (MBT-24H)
Contents:
  1. BBB Kit ? (MBT-24H)

  2. Thawing - and Incubation - Medium

  3. * Millicell? Hanging Cell Culture Insert  4
    (for Blank experiments)


货号品牌格式规格
价格
PCC-MBT-24HBBB Kit ?
(RBT-24H)
12 insertsSet25100元

* Millicell? is the Registered Trade Mark of Millipore Corporation.


Ready-To-Use BBB Kit ?

We deliver the complete set of BBB Kit ? in frozen packaged with dry ice. BBB Kit ? can be frozen as a whole and stored at -80℃. We guarantee to use BBB Kit ? (MBT-24H) within 1 month.
4 days prior to your experiment, you just thaw (de-freeze) your BBB Kit ? (MBT-24H) stored at -80℃ in your deep-freezer.


Amounts of solutions (to be added)
Thawing-solmedium (1)medium (2)  *
Blood-side200 μL300 μL300 μL
Brain-side1000 μL1200 μL1200 μL

* Recipe: medium 10% PDS / DMEM F-12

Dulbecco's Modified Eagle's Medium/ Nutrient Mixture F-12 Ham (DMEM F-12)
Fetal Bovine Plasma Derived Serum (PDS)10% (v/v)
Heparin100 μg/mL
basic fibroblast growth factor (bFGF)1.5 ng/mL
Insulin-Transferrin-sodium Selenite (ITS)insulin 5 μg/mL,
transferrin 5 μg/mL,
sodium selenite 5 ng/mL
Hydrocortisone500 nM
Gentamicin50 μg/mL

Expression of Tight Junctions (TJs) Proteins in BBB Kit? (MBT-24H)
claudin-5occludinZO-1


 Drug Permeability with BBB Kit? (MBT-24H)
permeability


Trans Endothelial Electrical Resistance (TEER) of BBB Kit ? (MBT-24H)
TEER in BBB Kit? (MBT-24H) reaches more than 150 Ω x cm2, and maintains a plateau up to 6 days. (Medium does not contain cAMP and its analogs.) TEER is measured at Day 3 after changing medium. BBB Kit? (MBT-24H) can be used from Day 4 to Day 6 after thawing.
【Equipment】


Insert Membrane Infomation
Insert of BBB Kit? (MBT-24H) (Polyethylene terephthalate membrance filter)
Millicell? Hanging Cell Culture Insert (#PISP 12R 48)
Technical data of the polyethylene terephthalate (PT )membrane Millicell? Insert (#PISP 12R 48)
Diameter:6.5 mm
Growth area:0.33 cm2
Membrane thickness:9 μm
Pore size:3.0 μm
Pore density:2 x 106 pores / cm2
Tissue culture treated:yes
Optical property:translucent
Cell visibility:poor
Volume inside insert:0.2 mL - 0.3 mL
Volume in plate:0.9 mL - 1.2 mL
Millicell? is the Registered Trade Mark of Millipore Corporation.


BBB Kit? ( MBT-24F )

BBB Kit? (MBT-24F) is constructed in 24 wells with Millicell? Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell? plate. [ F = Full ]
*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit? in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our Calculation of apparent permeability is Papp.
**Cells within BBB Kit? (MBT-24) are not clearly seen by microscopic observations.
***PT membrane hardly absorbs chemicals.

We deliver the complete set of BBB Kit? (MBT-24F) in frozen packaged with dry ice.
BBB Kit? can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit? (MBT-24F) within 1 month.

BBB Kit ? (MBT-24F)
Contents:
  1. BBB Kit ? (MBT-24F)

  2. Thawing - and Incubation - medium


货号品名格式规格价格
PCC-MBT-24F
(On-Demand Product)
BBB Kit ?
(MBT-24F)
24 insertsSet31000元




此外,世联博研公司还独家代理美国flocel 体外血脑屏障动力学实验仪器设备装置


美国flocel 体外血脑屏障动力学实验仪器设备装置,DIV-BBB,动态血脑屏障系统,跨细胞膜电阻测量(TEER 检测)系统,体外动态血脑屏障测量分析仪
型号:DIV-BBB
价格:请致电:010-67529703
品牌:美国flocel

Flocel体外三维动态血脑屏障系统

Flocel体外动态血脑屏障系统系统.jpg

Flocel体外动态血脑屏障系统系统
特点

  • 基于流的三维细胞培养装置模式,能更逼真模拟人体血脑屏障真实环境;

  • 可以做更准确的药代动力学和毒物学的研究

  • 更清楚准确的反应了体内血脑屏障的特性

  • 模拟重要的内皮细胞与星形胶质细胞之间的作用

  • 用电力方法检测了血脑屏障的完整性

  • 可以用真实的细胞做实验

  • 比现存的静态模型更能形成紧密的连接

  • 降低药物研发的成本

  • 计算机控制灌流泵,智能地调整液流

1.2 配置及参数资料
TEER检测系统 CARTRIDGE底板
4个DIV-BBB 盒子 检测软件 电缆线
DIV-BBB?管腔


特性

优点

可控制腔内/非腔体积比
体型小,仅2.57cm长
电极与管腔一体化
成本低

与体内体积比相符
减少了试验所需细胞数目
TEER检测系统操作简单
一次性使用,不能重复使用

疏水性毛细血管(Hydrophobic capillaries) Accurel PP Q3/2 (optionally pre-coated with ProNectin For Poly-D-Lysine)
经毛细管孔径: nominal 0.2um
中空纤维数量:19
腔内总体积 : 0.0123in3 = 0.202cm3
腔内表面积 : 2.09 in3= 13.5cm2
腔外体积 :0.070 in3 = 1.15cm3
腔外中空纤维总面积: 3.50 in3 = 22.6cm2
跨细胞膜电阻测量(TEER 检测)系统
TEER检测系统为研发人员提供了一个快速、简便地评估血脑屏障完整性方法,此外,电阻抗值(>1000 -cm2)也非常接近体内数值。TEER检测系统同时配带了所需检测软件、电线和衬板,可供4个DIV-BBB培养管腔同时使用。其中检测软件可以自定义设置泵的流速、频率、波形等参数。其它特性及优点见下表:


特性

优点

多频率下检测电阻抗

描述电极的电阻和电容

低电压,Max=60毫伏

限制对屏障的潜在破坏作用

自动检测多个管腔

可同时连接4个盒子

检测软件可自定义设置泵的流速、频率、波形等参数

检测电阻抗,记录数据

USB连接

台式或者便携式电脑均可连接

Flocel体外动态血脑屏障系统系统
计算机控制的脉动泵
脉搏率和作用周期完全模拟体内血液动力学设计,贴近体内环境,试验准确,脉搏率可设置在30-120/分范围内。其特性和优点如下:


特性

优点

4个管腔的流速和泵的速度可分别控制,

允许4个独立试验

外部24电压墙与电源连接

减少培养箱中高温

接受来自TEER检测系统的指定程序

不需要额外的USB接口

泵速: 30-120bpm
流速: 1-50ml/min
电源: TEER控制

系统组成部件:

  • 白色系统底托(White plastic tray)

  • 4 个通道体外细胞培养系统(4 DIV-BBB cartridges)及配套硅胶管

  • 跨上皮细胞电阻测量系统(TEER Measurement System )/泵控制电缆(Pump Control Cable)

  • 4套125毫升培养基玻璃瓶(4 glass 125ml bottles)及4套瓶盖(4 bottle caps)

  • 4套33毫米的储液杯及盖子 (4 33mm Reservoir Caps)

  • 计算机控制的Quad程控脉冲式灌流泵(Flocel Qual Computer-controlled Perfusion System)及配套硅胶管

  • 8个鲁尔锁栓 (8 male luer locks)

  • 4个三通阀 (4 3-way stopcocks)

  • USB接口线 (USB cable)

  • 电源线 (Power supply cable)

  • Flocel控制测量系统软件(Flocel System Installation Software)

  • 用户使用手册 (User’s Manual)

Publications 文献

1.The role of shear stress in Blood-Brain Barrier endothelial physiology.
Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D.

2.>A dynamic in vitro BBB model for the study of immune cell trafficking into the central nervous system.
Cucullo L, Marchi N, Hossain M, Janigro D.

3.>Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow.Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N.

4.>Tobacco smoke: a critical etiological factor for vascular impairment at the blood-brain barrier.
Hossain M, Sathe T, Fazio V, Mazzone P, Weksler B, Janigro D, Rapp E, Cucullo L.

5.>Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies.
Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D.

6.>Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: a permeability study.
Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L.

7.>Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic drugs.
Cucullo L, Hossain M, Rapp E, Manders T, Marchi N, Janigro D.

8.>Drug delivery and in vitro models of the blood-brain barrier.
Cucullo L, Aumayr B, Rapp E, Janigro D.

9.Glycerophosphoinositol and dexamethasone improve transendothelial electrical resistance in an in vitro study of the blood-brain barrier.
Cucullo L, Hallene K, Dini G, Dal Toso R, Janigro D.

10.>Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis.
Marroni M, Kight KM, Hossain M, Cucullo L, Desai SY, Janigro D.

11.>A new dynamic in vitro model for the multidimensional study of astrocyte-endothelial cell interactions at the blood-brain barrier.
Cucullo L, McAllister MS, Kight K, Krizanac-Bengez L, Marroni M, Mayberg MR, Stanness KA, Janigro D.

12.>Mechanisms of endothelial survival under shear stress.
Desai SY, Marroni M, Cucullo L, Krizanac-Bengez L, Mayberg MR, Hossain MT, Grant GG, Janigro D.

Posters

1.A Dynamic Humanized drug resistant In Vitro Blood-Brain Barrier Model to assess the permeability of relevant CNS drugs

2.Development of a novel blood-brain barrier model by coupling Immortalized human brain endothelial cells with flow-based cell culture technologies

3.Glioblastoma Cell Line Metabolism Under Dynamic and Static Conditions: Effects on tumor growth and BBB integrity

4.Humanized in vitro blood-brain barrier models to screen for brain penetration of antiepileptic drugs

5.Effect of Shear stress on BBB endothelial cells: A proteomic study by 2-dimensional protein electrophoresis