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现在位置首页>行业专用>生命科学仪器>生命科学仪器>MBT-24H,MBT-24F药品血脑屏障渗透性研究套件(由小分子到细胞)
MBT-24H,MBT-24F药品血脑屏障渗透性研究套件(由小分子到细胞)
MBT-24H,MBT-24F药品血脑屏障渗透性研究套件(由小分子到细胞)
  • MBT-24H,MBT-24F药品血脑屏障渗透性研究套件(由小分子到细胞)

MBT-24H,MBT-24F药品血脑屏障渗透性研究套件(由小分子到细胞)

产品报价:询价

更新时间:2019/4/2 12:09:54

地:德国

牌:pharma-cocell

号: MBT-24H,MBT-24F

厂商性质: 生产型,贸易型,服务型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

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BBB Kit? ( MBT-24H ) / ( MBT-24F )*
For Drug BBB Permeability Study ( from micromolecules to cells )



BBB Kit? (MBT-24) is a new in vitro model of the blood-brain barrier (BBB) made of primary cultures ofmonkey (Macaca irus) brain capillary endothelial cells, brain pericytes and astrocytes. BBB Kit? (MBT-24) with a polyethylene terephthalate (PT) membrane is a ready-to-use concept for your evaluations of BBB-permeability of drugs and drug candidates.
BBB Kit? (MBT-24H) is a 12-well format constructed in a 24-well Millicell? cell culture plate.
( *On-Demand Product - MBT-24F )


*Brain capillary endothelial cells are isolated from monkey (Macaca irus) brain, and brain pericytes and astrocytes are from rat (Wistar rat) brain.
BBB Kit? (MBT-24H) is constructed in 12 wells (B and C) with Millicell? Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell? plate. [ H = Half ]
*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit? in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our calculation of apparent permeability is Papp.
**Cells within BBB Kit? (MBT-24) are not clearly seen by microscopic observations.
***PT membrane hardly absorbs chemicals.

We deliver the complete set of BBB Kit? (MBT-24H) in frozen packaged with dry ice.
BBB Kit? can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit (MBT-24H) within 1 month.

BBB Kit ? (MBT-24H)
Contents:
  1. BBB Kit ? (MBT-24H)

  2. Thawing - and Incubation - Medium

  3. * Millicell? Hanging Cell Culture Insert  4
    (for Blank experiments)

货号品牌格式规格
价格
PCC-MBT-24HBBB Kit ?
(RBT-24H)
12 insertsSet25100元

* Millicell? is the Registered Trade Mark of Millipore Corporation.


Ready-To-Use BBB Kit ?

We deliver the complete set of BBB Kit ? in frozen packaged with dry ice. BBB Kit ? can be frozen as a whole and stored at -80℃. We guarantee to use BBB Kit ? (MBT-24H) within 1 month.
4 days prior to your experiment, you just thaw (de-freeze) your BBB Kit ? (MBT-24H) stored at -80℃ in your deep-freezer.


Amounts of solutions (to be added)
Thawing-solmedium (1)medium (2)  *
Blood-side200 μL300 μL300 μL
Brain-side1000 μL1200 μL1200 μL

* Recipe: medium 10% PDS / DMEM F-12
Dulbecco's Modified Eagle's Medium/ Nutrient Mixture F-12 Ham (DMEM F-12)
Fetal Bovine Plasma Derived Serum (PDS)10% (v/v)
Heparin100 μg/mL
basic fibroblast growth factor (bFGF)1.5 ng/mL
Insulin-Transferrin-sodium Selenite (ITS)insulin 5 μg/mL,
transferrin 5 μg/mL,
sodium selenite 5 ng/mL
Hydrocortisone500 nM
Gentamicin50 μg/mL

Expression of Tight Junctions (TJs) Proteins in BBB Kit? (MBT-24H)
claudin-5occludinZO-1

 Drug Permeability with BBB Kit? (MBT-24H)
permeability

Trans Endothelial Electrical Resistance (TEER) of BBB Kit ? (MBT-24H)
TEER in BBB Kit? (MBT-24H) reaches more than 150 Ω x cm2, and maintains a plateau up to 6 days. (Medium does not contain cAMP and its analogs.) TEER is measured at Day 3 after changing medium. BBB Kit? (MBT-24H) can be used from Day 4 to Day 6 after thawing.
【Equipment】


Insert Membrane Infomation
Insert of BBB Kit? (MBT-24H) (Polyethylene terephthalate membrance filter)
Millicell? Hanging Cell Culture Insert (#PISP 12R 48)
Technical data of the polyethylene terephthalate (PT )membrane Millicell? Insert (#PISP 12R 48)
Diameter:6.5 mm
Growth area:0.33 cm2
Membrane thickness:9 μm
Pore size:3.0 μm
Pore density:2 x 106 pores / cm2
Tissue culture treated:yes
Optical property:translucent
Cell visibility:poor
Volume inside insert:0.2 mL - 0.3 mL
Volume in plate:0.9 mL - 1.2 mL
Millicell? is the Registered Trade Mark of Millipore Corporation.


BBB Kit? ( MBT-24F )

BBB Kit? (MBT-24F) is constructed in 24 wells with Millicell? Hanging Cell Culture Insert (#PISP 12R 48) in a 24-well Millicell? plate. [ F = Full ]
*For evaluating the permeability of a molecule through the brain endothelial and pericytes layers of BBB Kit? in blood-to-brain direction, the molecule is applied to the upper compartment of the insert. Our Calculation of apparent permeability is Papp.
**Cells within BBB Kit? (MBT-24) are not clearly seen by microscopic observations.
***PT membrane hardly absorbs chemicals.

We deliver the complete set of BBB Kit? (MBT-24F) in frozen packaged with dry ice.
BBB Kit? can be frozon as a whole and stored at -80℃. We gurantee to use BBB Kit? (MBT-24F) within 1 month.

BBB Kit ? (MBT-24F)
Contents:
  1. BBB Kit ? (MBT-24F)

  2. Thawing - and Incubation - medium

货号品名格式规格价格
PCC-MBT-24F
(On-Demand Product)
BBB Kit ?
(MBT-24F)
24 insertsSet31000元