Flocel体外三维动态血脑屏障系统

特点

• 基于流的三维细胞培养装置模式，能更逼真模拟人体血脑屏障真实环境；

• 可以做更准确的药代动力学和毒物学的研究

• 更清楚准确的反应了体内血脑屏障的特性

• 模拟重要的内皮细胞与星形胶质细胞之间的作用

• 用电力方法检测了血脑屏障的完整性

• 可以用真实的细胞做实验

• 比现存的静态模型更能形成紧密的连接

• 降低药物研发的成本

• 计算机控制灌流泵，智能地调整液流

1.2 配置及参数资料
TEER检测系统 CARTRIDGE底板
4个DIV-BBB 盒子 检测软件 电缆线
DIV-BBB? 管腔

疏水性毛细血管(Hydrophobic capillaries) Accurel PP Q3/2 (optionally pre-coated with ProNectin For Poly-D-Lysine)
经毛细管孔径： nominal 0.2um
中空纤维数量：19
腔内总体积 ： 0.0123in³ = 0.202cm³
腔内表面积 ： 2.09 in³= 13.5cm²
腔外体积 ：0.070 in³ = 1.15cm³
腔外中空纤维总面积： 3.50 in³ = 22.6cm²
跨细胞膜电阻测量（TEER 检测）系统
TEER检测系统为研发人员提供了一个快速、简便地评估血脑屏障完整性方法，此外，电阻抗值（＞1000 -cm²）也非常接近体内数值。TEER检测系统同时配带了所需检测软件、电线和衬板，可供4个DIV-BBB培养管腔同时使用。其中检测软件可以自定义设置泵的流速、频率、波形等参数。其它特性及优点见下表：

计算机控制的脉动泵
脉搏率和作用周期完全模拟体内血液动力学设计，贴近体内环境，试验准确，脉搏率可设置在30-120/分范围内。其特性和优点如下：

泵速： 30-120bpm
流速： 1-50ml/min
电源： TEER控制

系统组成部件：

• 白色系统底托（White plastic tray）

• 4 个通道体外细胞培养系统（4 DIV-BBB cartridges）及配套硅胶管

• 跨上皮细胞电阻测量系统（TEER Measurement System ）/泵控制电缆(Pump Control Cable)

• 4套125毫升培养基玻璃瓶（4 glass 125ml bottles）及4套瓶盖（4 bottle caps）

• 4套33毫米的储液杯及盖子 （4 33mm Reservoir Caps）

• 计算机控制的Quad程控脉冲式灌流泵(Flocel Qual Computer-controlled Perfusion System)及配套硅胶管

• 8个鲁尔锁栓 （8 male luer locks）

• 4个三通阀 （4 3-way stopcocks）

• USB接口线 （USB cable）

• 电源线 （Power supply cable）

• Flocel控制测量系统软件（Flocel System Installation Software）

• 用户使用手册 （User’s Manual）

Publications 文献

1.The role of shear stress in Blood-Brain Barrier endothelial physiology.
Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D.

2.>A dynamic in vitro BBB model for the study of immune cell trafficking into the central nervous system.
Cucullo L, Marchi N, Hossain M, Janigro D.

3.>Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow.Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N.

4.>Tobacco smoke: a critical etiological factor for vascular impairment at the blood-brain barrier.
Hossain M, Sathe T, Fazio V, Mazzone P, Weksler B, Janigro D, Rapp E, Cucullo L.

5.>Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies.
Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D.

6.>Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: a permeability study.
Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L.

7.>Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic drugs.
Cucullo L, Hossain M, Rapp E, Manders T, Marchi N, Janigro D.

8.>Drug delivery and in vitro models of the blood-brain barrier.
Cucullo L, Aumayr B, Rapp E, Janigro D.

9.Glycerophosphoinositol and dexamethasone improve transendothelial electrical resistance in an in vitro study of the blood-brain barrier.
Cucullo L, Hallene K, Dini G, Dal Toso R, Janigro D.

10.>Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis.
Marroni M, Kight KM, Hossain M, Cucullo L, Desai SY, Janigro D.

11.>A new dynamic in vitro model for the multidimensional study of astrocyte-endothelial cell interactions at the blood-brain barrier.
Cucullo L, McAllister MS, Kight K, Krizanac-Bengez L, Marroni M, Mayberg MR, Stanness KA, Janigro D.

12.>Mechanisms of endothelial survival under shear stress.
Desai SY, Marroni M, Cucullo L, Krizanac-Bengez L, Mayberg MR, Hossain MT, Grant GG, Janigro D.

Posters

1.A Dynamic Humanized drug resistant In Vitro Blood-Brain Barrier Model to assess the permeability of relevant CNS drugs

2.Development of a novel blood-brain barrier model by coupling Immortalized human brain endothelial cells with flow-based cell culture technologies

3.Glioblastoma Cell Line Metabolism Under Dynamic and Static Conditions: Effects on tumor growth and BBB integrity

4.Humanized in vitro blood-brain barrier models to screen for brain penetration of antiepileptic drugs

5.Effect of Shear stress on BBB endothelial cells: A proteomic study by 2-dimensional protein electrophoresis