产品简介:
DAPI,DAPI是一种可以穿透细胞膜的蓝色荧光染料,其最大激发波长为340nm,最大发射波长为488nm;DAPI和双链DNA结合后,最大激发波长为364nm,最大发射波长为454nm。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。
DAPI, 和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI,DAPI发射的蓝光,可以便捷地用于多重荧光分析中;因为很少有荧光能在DAPI和绿色荧光分子(例如荧光素和绿色荧光蛋白GFP;或者红色荧光染料,像Texas Red)产生重叠。除了标记细胞核素外,DAPI也可以用来检测细胞培养物中的支原体或者病毒DNA。DAPI dilactate与DAPI HCl盐相比具有更好的水溶性。
英文注释:
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.
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