SymCel代理,calScreener细胞能量代谢实时检测系统
产品报价:询价
更新时间:2023/4/3 18:11:18
产地:其他国家
品牌:瑞典SymCel
型号:calScreener
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2023/4/3 18:11:18
产地:其他国家
品牌:瑞典SymCel
型号:calScreener
厂商性质: 生产型,
公司名称: 世联博研(北京)科技有限公司
王荣 : (18618101725) (18618101725)
(联系我时,请说明是在来宝网上看到的,谢谢!)calScreener高灵敏微量热法无标记实时多通道细胞能量代谢监测系统 | ||||||||||||||||||||||||||||||
概述: 利用活体细胞热流测量监测代谢变化是一个成熟完善的技术。瑞典symcel利用其微量热量检测分析专利技术, 发明了业界第一款多通道、高灵敏、无需标记的CalScreener细胞代谢和生物能量实时连续监测分析系统,集高灵敏度和优异的基线稳定性于一身。 该系统可以进行包括细胞代谢分析、氧呼吸测定、药物代谢分析、线粒体有氧代谢和糖酵解等功能的细胞代谢和生物能力所有类型的变化。
线粒体是细胞中极为重要的细胞器。在生命体能量代谢过程中,除一部分能量用于ATP 合成外,其余则以热的 —病变癌症细胞热功率大于正常细胞热功率. calScreener采集连续的数据流,以促进动力学行为研究,比如细胞生长或者凋亡。连续读出方式更容易找到感兴趣的时间点用来测量细胞活动。 1.1生物利用度—你的化合物能够影响活体细胞吗? 2.1高产克隆鉴定 3.1研究过程早期识别毒性事件 4.1代谢监测 calPlate包括单个密封罩杯,用于保持培养细胞放置于一个恒温室装置中,且目标温度值设置精度在几千开尔文之内。 所有产生的热量将被转移至散热片,从而引起热电偶传感器信号正比例于热流通量。 因此,所测热量是独立于相关的模型系统或过程。我们拥有一个无标记、实时探测系统,可以应用于更广泛的生物系统检测领域。 比如制药生产中蛋白过表达。calScreener是基于培养细胞的热反应测量,且不依赖于任一具体或繁琐的试验配方。 由于无须添加底物或事先了解具体途径和靶目标,因此,将大幅地缩减实验结果的时间及获得实验数据的花费。 calScreener可以检测到代谢率变化,并提供一个可直接测量细胞过程的方法,特别是无需了解代谢途径的先验知识 或物质效应筛选的作用机理。 基于三维培养的球形矩阵可用于研究细胞反应和生物能量学。
细胞分析想法和必要条件。
calPlate设计符合标准的微量滴定板格式。系统先进的标准化设计风格很适合细胞生长与热量测量的两者需求。 calPlate系统不仅使用方便、节约成本,而且同时可最大限度地减少环境负荷。 calplate样品容器池适合粘壁细胞测试如肌肉、脂肪、肾脏、卵巢、肝脏等粘壁细胞。 48孔格式可同时对32个样本细胞以2×8位置进行测量,以便用作热量测量参考以提升测量灵敏度和性能。
并可同时观察所有试验过程的图形窗口,以及单项试验图的近视图。
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calScreener? is the first multi-channel calorimetric system specifically developed for cell based assays. Measurement of heat flow in living cells is a well established and proven technology to monitor changes in metabolic processes. calScreener? provides the researcher with a label-free detection tool with maximal versatility in assay type.
The real time continuous measurement provides important kinetic data for interpretation of cellular mechanisms and responses to treatment.
calScreener? is suited to drug development and metabolic research providing a true phenotype response where the total status of the cell metabolism is monitored.
calScreener? is furthersuitable for toxicology tests, process development and environmental monitoring.
calScreener? is uniquely suited to the emerging interest in monitoring the response and behaviour of three dimensional cell cultures. The label-free system is completely independent of sample morphology. Cellular response and bioenergetics can be studied in real-time for spherical and matrix based three dimensional cultures.
First Calorimeter designed for cell biological work
Label-free assay
Independent of cell morphology
Measures heat produced from cell metabolism
True PHENOTYPE response measured
Real-Time continuous measurement
Prior knowledge of pathways and specific target function not needed
Multiple compounds can be tested simultaneously for synergetic effects without knowledge of pathway interactions
Suitable for a wide range of applications
The versatility and speed makes calScreener? suitable for a wide range of metabolic assay applications. calScreener? detects changes in metabolic rate induced by addition of chemical- or biological compounds and provides a direct measurement of the resulting cellular processes. There is no need to have prior knowledge of metabolic pathways or mechanism of action to screen the effects of substances.
calScreener? Key Features:
48 well standard plate size adapted format
Cell type & cell morphology independent
Target independent
Medium throughput format
The calScreener? system from SymCel provides researchers with a novel tool to measure the cellular metabolic response in a native environment.
calScreener? is based on the measurement of thermogenic response in cultivated cells and is not dependent on any specific or cumbersome assay formulation. Since no addition of substrates or knowledge of the specific pathway or target is needed the time to results is greatly reduced as well as the cost for obtaining data.
The label-free principle of obtaining data makes calScreener? suitable for a wide range of cell-science applications. calScreener? can be used a screening tool applicable to different biological systems. Any types of cultivated cells as well as yeast or bacterial cultures are possible to use. calScreener? measures thermal power which directly describes the metabolic rate. Both anabolic and catabolic processes are monitored.
The calorimetry principle is based on measuring the heat production changes induced by changes in the metabolism by chemical intervention of biological processes. Also other types of induced processes such as over-expression of proteins for pharmaceutical production, creates changes in the thermal output and can thus be monitored.
calScreener? can be used for several types of applications where changes in metabolic rate are expected e.g. compound screening, lead substance optimization, bioavailability studies, cellular toxicity studies, biopharmaceutical process optimization, and antibiotics development.
calScreener? is a complete assay solution with integration all the way from cell culture consumables to the measurement step and finally data analysis software.
The first multi-channel calorimeter designed for cell-biological work.
Measures direct heat flow from cell metabolism
Label-free assay – no radioactivity, antibodies, fluorescence
Minimal assay development needed
Minimize time and consumables for experiments
Real-time continuous kinetic data
Adaptable for a large number of different Cell Biology Applications
The new calScreener? enables all scientist access to advanced calorimetric monitoring with ease of use. The instrument is designed to use the calPlate? microtiter plate system for cell growth and assays.
Label free, real-time detection
32 simultaneous cell samples
Detection limit better than 50 nW; well within the limits since a typical thermal output from a culture of cells this size is in the range of 3 – 10 mW.
calPlate? system
calPlate? system is a tailor-made series of consumables for calScreener? to ensure correct performance of the system. The design of the calPlate? conforms to the standard micro titer plate format as sample containers. The advanced modular design of the system is developed to suit both the needs of cell growth as well as the requirements of calorimetric measurement. The calPlate? system is both easy to use, cost effective and at the same time minimizing the environmental load. The plastic system is suitable for tests where adhesive cells, i.e. muscle, fat, kidney, ovary, liver etc, are used. The 48 well format allows the simultaneous measurement of 32 cell-samples as 2 x 8 positions are used as a calorimetric reference for increased sensitivity and performance.
calView? data collection software
An in-house developed software with a user-friendly graphical interface. It allows the user to assign real-time data for each test well and view the progress of the all test graphs simultaneously as well as close-up view of individual test graphs. The software is designed to secure data traceability.
calResult? data analysis software
calResults? is based on the Origin Pro-software for scientific and engineering data analysis and data presentation. The data obtained in CalView is imported and analyzed in to the CalResults module for analysis. Customized methods can be developed and incorporated into the CalResult? application.
The 'cal' part of our product name is an identity label derived from calorimetry -pointing to the origin of the technology. 'cal' is also part of the word calor -Latin for heat.
The calScreener?, calPlate?, calView? & calResults? are all trademarks of Symcel Sverige AB 2010.
All types of changes in the cellular metabolism can be monitored since calScreener? gives a measurement of the combined changes in metabolic rate. A majority of cellular processes such as cell death and cell proliferation affects the cellular metabolism to a large degree. More subtle processes such as cell signaling also give rise to changes in metabolic turnover. The use of a calorimetric methodology to detect major, as well as, subtle cellular events is a well established methodology. The SymCel calScreener? is a high sensitivity, easy to use calorimeter designed for cell biological work, opening up a new set of opportunities for research scientists and drug development.
SymCel calScreener? uses no label –no additives.
This results in faster assay development and cost savings since there are no reagents involved. The assay maintains the native environment of the cell and errors introduced by additions of antibodies or other reagents are avoided. The use of heat production measurement to monitor cell metabolism is also a non-destructive method facilitating further downstream analysis of cells for e.g. RNA expression levels. There is no need to have prior knowledge of a specific drug-target or the involved pathways or reaction mechanism.
Many of the current available cell-biological analysis technologies depend on ‘end-point’ measurements where data is derived from a specific time point (e.g. reporter gene assays). calScreener? collects a continuous data-stream facilitating research of kinetic behavior such as cell growth or apoptosis. The continuous read-out makes it easier to find the interesting time-points for measuring cellular events.
The instrument was initially developed to analyze adherent eukaryotic cells but may be used for any cell type suspended or adherent, including bacterial cells and yeast.
Using a suitable cell-model calScreener? is suitable for toxicology applications. The most common experiment would involve the comparison of the metabolic response of a novel compound with different know toxicological standards.
calScreener? is currently being tested by a select number of labs to obtain user feedback and peer-reviewed scientific publications. The goal is to be able to offer a fully commercial product by Q2 2013
Measuring the metabolic activities in living organisms is a well established science. In 1784, Antoine Laurent Lavoisier and Pierre Simon de Laplace cleverly devised the first calorimetric device, using heat to measure chemical and physical changes. Calorimeters have evolved to become a modern tool for the advancement of science. Large volume single channel calorimeters have found wide spread applications in the industry, mainly in material, chemical and pharmaceutical companies.
SymCel is introducing the first calorimeter developed specifically for cell-based assays suitable for both advanced metabolic research as well as drug discovery and development applications.
calScreener? technology is valid for monitoring changes in biological processes caused by physical, chemical or biological stimuli. Changes in metabolic activity will cause changes in heat dissipated from the cell, tissue or organism.
Depending on the biological process involved different kinetic behaviors are anticipated. The graphs below are idealized examples of the different heat output over time from different cellular processes.
Drug development
Bioavailability – Are your compounds able to affect living cells?
Target validation
Hit validation; rapid assessment of effect on cells
Rapid filtering of hit compounds with in-built toxicity testing
Lead selection
Protein Production
Identification of High-producing Clones
Optimization of Culture Conditions
Toxicology
Identify toxicological events at early stage in the discovery process
Basic Research
Metabolic monitoring
Proliferation assays
Other
calScreener? is not limited to these few applications. The application areas are limited only by the imagination of the scientist. We strongly encourage you to discuss with us your label-free cell-assay ideas and requirements.
Biological processes caused by physical, chemical or biological stimuli in which metabolic changes are anticipated are all valid for the analysis.
The calPlate? containing the individual sealed cups holding the cell culture are placed in a thermostatic chamber set at the target temperature with a precision within thousands of a Kelvin.
The cups rest upon a heat-flux detecting sensor, the thermopile. The sensor is attached to a heat-sink with a large mass compared to the cell-culture cups. All heat produced is transferred to the heat-sink giving rise to a signal in the thermopile sensor proportional to the heat-flow.
The measured heat is thus independent of the model system or the process involved. We have a label free, real-time, detection system applicable to a wide range of biological applications
Below are some publication examples of biological processes and applications where heat measurements have been conducted using calorimetric equipment, including measurement of basic cellular responses such as cell proliferation, cell death (apoptosis) and cell signaling.
Apoptosis
Apoptotic processes are manifested by a typical heat pattern when DNA is fragmented
Bermudez, J., P. Backman, et al. (1992). “Microcalorimetric evaluation of the effects of methotrexate and 6-thioguanine on sensitive T-lymphoma cells and on a methotrexate-resistant subline.” Cell Biophys. 20(2-3): 111-23.
Wallen-?hman, M., P. L?nnbro, et al. (1993). “Antibody-induced apoptosis in a human leukemia cell line is energy dependent: thermochemical analysis of cellular metabolism.”Cancer Letters 75(2): 103-9.
Roig, T. and J. Bermudez (1995). “Microcalorimetric evaluation of the effect of combined chemotherapeutic drugs.” Biochim Biophys Acta. 1244(2-3): 283-90.
Bluthnerhassler, C., M. Karnebogen, et al. (1995). “Influence of Malignancy and Cyctostatic Treatment on Microcalorimetric Behavior of Urological Tissue Samples and Cell-Cultures.”Thermochimica Acta 251: 145-154.
Thermogenesis
B?ttcher, H. and P. Fürst (1996). “Microcalorimetric and biochemical investigations of thermogenesis and metabolic pathways in human white adipocytes.” Int J Obes Relat Metab Disord. 20(9): 874-81.
Hinz, W., B. Faller, et al. (1999). “Recombinant human uncoupling protein-3 increases thermogenesis in yeast cells.” FEBS Lett. 448(1): 57-61.
Growth
Feng, Y., S. F. Luo, et al. (1997). “Study on the thermosensitivity of a tumor cell by microcalorimetry.” Thermochimica Acta 303(2): 203-207.
Andlid, T., L. Blomberg, et al. (1999). “Characterization of Saccharomyces cerevisiae CBS 7764 isolated from rainbow trout intestine.” Systematic and Applied Microbiology 22(1): 145-155.
Barros, N., S. Feijoo, et al. (2001). “Interpretation of the metabolic enthalpy change, DHmet, calculated for microbial growth reactions in soils.” Journal of Thermal Analysis and Calorimetry 63(2): 577-588.
Dejean, L., O. Bunoust, et al. (2002). “Control of growth yield of yeast on respiratory substrate by mitochondrial content.” Thermochimica Acta 394(1-2): 113-121.