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全内反射荧光显微镜,Total Internal Reflection Fluorescence Microscopy
全内反射荧光显微镜,Total Internal Reflection Fluorescence Microscopy
  • 全内反射荧光显微镜,Total Internal Reflection Fluorescence Microscopy

全内反射荧光显微镜,Total Internal Reflection Fluorescence Microscopy

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:tirf-labs

号:TIRFM显微镜

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

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1. TIRF MICROSCOPY

TIRF Labs offers a family of TIRF Microscopy systems with different geometries:

prism-based, lightguide-based, and objective-based TIRFM. Each geometry has its own

set of advantages and limitations. For example, p-TIRFM provides the best signal-to

background ratio and is well-suited for in vitro studies with closed flow cell surrounding

the TIRF area, but is too difficult to implement p-TIRFM with open perfusion chamber on

an inverted microscope. lg-TIRFM and o-TIRFM are better-suited for live cell studies with

open perfusion chambers. Both p- and lg-TIRFM are factory-aligned systems with highly

reproducible intensity of the evanescent wave; they are well-suited for multicolor TIRF

experiments (see Application Notes at www.tirf-labs.com). o-TIRFM requires alignment,

experiences irreproducible intensity of the evanescent wave, but allows to vary the angle

of incidence, and hence, the depth of penetration. All three TIRFM geometries efficiently

reject background fluorescence and scatter and are capable of super sensitive detection,

down to single molecules. Selecting of geometry and particular configuration of TIRF

system depends on your application. TIRF Labs will help you in this process. Please read

the brochure “Compare TIRF Geometries” and TIRFM application notes, and email or call

us for our recommendation for TIRF geometry and specific configuration


1.1. p-TIRFM - prism-based Total Internal Reflection Fluorescence Microscopy. p-TIRFM is

a geometry, which provides the best signal-to-background ratio. TIRF Labs offers p-TIRFM flow

system with closed flow cell and advanced fluidics, which is well-suited for handling small liquid

aliquots, down to sub-microliter volumes. p-TIRFM can be used

with dry, water-, and oil-immersion objectives. It uses silica

prism and silica or glass slides. p-TIRFM is a factory aligned

system supplied as an add-on accessory for rapid installation on

inverted microscopes. p-TIRFM fits into 128 mm x 85 mm

window that holds 96-well SBS plate. It can be used with Ludl

and Prior motorized stages, as well as manual XY-translation

stages. Base model is supplied with one of the illuminators: 405 nm, 450 nm, 532 nm, or 630 nm

installed at the p-TIRFM platform. Multicolor illuminators are available as options.

1.2.1 lg-TIRFM-170 - lightguide-based Total Internal Reflection Fluorescence Microscopy

is a geometry, which exhibits excellent signal-to-background ratio, close to that of p-TIRFM

geometry. Similar to p-TIRFM, the excitation light does not travel through the emission channel,

providing to lg-TIRFM signal-to-background ratio an order of magnitude better than that for o-

TIRFM. lg-TIRFM is a factory-aligned system with fixed angles and reproducible intensity of the

evanescent wave, which is well-suited for multicolor TIRFM experiments. lg-TIRFM can be used