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现在位置首页>实验试剂制品>抗体>离子通道抗体>Alomone现货促销 Anti-Calcium Sensing Receptor (extracellular) ACR-004
Alomone现货促销 Anti-Calcium Sensing Receptor (extracellular) ACR-004
Alomone现货促销 Anti-Calcium Sensing Receptor (extracellular) ACR-004
  • Alomone现货促销 Anti-Calcium Sensing Receptor (extracellular) ACR-004

Alomone现货促销 Anti-Calcium Sensing Receptor (extracellular) ACR-004

产品报价:5135元

更新时间:2013/6/17 16:49:51

地:其他国家

牌:Alomone

号: ACR-004

厂商性质: 生产型,贸易型,服务型,

公司名称: 北京乐博生物科技有限公司

产品关键词:

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General information

The Calcium-Sensing Receptor (CaSR) is a member of subfamily C of theG-protein-coupled receptor (GPCR) superfamily. As its primary role, theCaSR is a key player in calcium homeostasis and is expressed in tissuesinvolved in calcium metabolism, such as parathyroid cells and kidney.1,2

The expression of CaSR was also described in other cell types andtissues, such as neurons, keratinocytes, and pancreas. However, the roleof CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elementsin the CaSR gene, it was suggested that vitamin D might regulate theexpression of CaSR.3
 
CaSR activation is followed by a rapid, transient increase in thecytosolic calcium concentration, resulting from mobilization of calciumfrom thapsigargin-sensitive intracellular stores, as well as anincreased calcium influx through voltage-insensitive calcium channelslocated at the cell membrane.4

CaSR regulates cellular processes such as proliferation, apoptosis, anddifferentiation under both normal and pathologic conditions.5

Recent studies demonstrated the expression in human colon epithelium ofCaSR, which regulates proliferation and differentiation. In coloncarcinomas,lower levels of expression were found in compared to normalcolon tissue.

Alomone Labs is pleased to offer a highly specific antibody directedagainst an extracellular epitope at the N-terminus of human CaSR. Anti-Calcium SensingReceptor (extracellular) antibody (#ACR-004) can be used in western blot analysis andimmunocytochemistry applications, and recognizes CaSR in human, rat andmouse samples.

Applications
Specifications
References
Product Citation

Western blot
Western blot analysis of Colo205 (lanes 1and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates:
1,2,5. Anti-Calcium Sensing Receptor (extracellular) antibody (#ACR-004), (1:200).
3,4,6. Anti-Calcium Sensing Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Immunocytochemistry
Expression of CaSR in C6 cells 
Immunocytochemical staining of paraformaldehyde-fixed and permeabilized C6 (rat brain glioma) cells. A. Staining using Anti-Calcium Sensing Receptor (extracellular) antibody (#ACR-004), (1:50), followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using Hoechst 33342. C. Merged images of panels A and B.
Indirect flow cytometry
Indirect Flow cytometry analysis of live Jurkat (human acute T cell leukemia) cells:
___ Unstained cells.
___ Cells + Anti-Calcium Sensing Receptor (extracellular) antibody (#ACR-004), (10 µg/5x105 cells).