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现在位置首页>行业专用>生命科学仪器>细胞培养系统>Z® RP Technology ,Zellwerk 全自动三维细胞及组织培养系统
Z® RP Technology ,Zellwerk 全自动三维细胞及组织培养系统
Z® RP Technology ,Zellwerk 全自动三维细胞及组织培养系统
  • Z® RP Technology ,Zellwerk 全自动三维细胞及组织培养系统

Z® RP Technology ,Zellwerk 全自动三维细胞及组织培养系统

产品报价:询价

更新时间:2019/4/2 12:09:54

地:德国

牌:Zellwerk

号:Z® RP Technology

厂商性质: 生产型,贸易型,服务型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

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The Z® RP cell- and tissue culturing systems establish a unique technological platform for fast and controlled expansion of adherent cells. Complying with GLP, GCP, GTP and GMP standards Z® RP culturing systems enable users to grow cells in very high densities, inducing tissue-like organisation of cells embedded in their own extracellular matrix.
Zellwerk's Z® RP technology opens up completely new perspectives for regenerative medicine being the ideal tool for expansion and harvesting of primary cells and stem cells. The scope of therapeutical applications also includes tissue engineering and even production of complete implants made from colonized scaffolds. Moreover, the compact Z® RP cultivation system allows cost-efficient production of recombinant biopharmaceuticals, vaccines, vectors e. c. Researchers profit from the variability in culturing configurations speeding up development projects significantly.

Z® RP enables advanced...

  • fast and safe expansion of adherent cells
  • three-dimensional high density cultivation in cell-specific extracellular matrix
  • expansion and harvesting of viable primary cells for therapeutical applications
  • expansion of stem cells with control of differentiation status
  • colonization of scaffolds for implant generation
  • long-term culturing of product secreting cell
  • efficient production of recombinant proteins and vaccines
  • production of excellently glycosylated proteins
     
A Z® RP cultivation system comprises of Z® RP Bioreactor, Z® RP GMP Breeder and Z® RP Control Unit. Conducted in the sterile environment of a Z® RP GMP Breeder manifold types of cell and tissue culturing processes are feasible.
Z® RP bioreactors are easy to assemble and handle. They are usually operated in perfusion mode and host large amounts of cells in very small volumes. The centrepiece is a magnetic coupled rotating axis mounted with the cell- or tissue carrier of choice exposing cells to medium and overlay alternately.
From highly porous Sponceram® discs to implant scaffolds all kinds of supports can be installed in a Z® RP bioreactor giving rise to a vast variety of culturing options. In all configurations best possible aeration and feeding is guaranteed. The gentle rotational motion stimulates cells and tissues to adhere and proliferate fast without being stressed by shear forces. Cell populations stay viable and express large amounts of extracellular matrix. Three-dimensional high density cultivation can be extended to many months without loosing viability or expression productivity. Harvest of adherent cells is easily achieved employing specific rotation programs in combination with detaching solutions.
Alternative rotating bed for implant blanks
A Z® RP GMP Breeder is an innovative supply system combined with a laminar flow functionality. The sterile and temperature controlled workroom protects the Z® RP bioreactor from contamination and enables the user to conduct all necessary manipulations - e. g. preparation of cell and tissue samples, inoculation, change of medium container, tube change, sensor exchange, cell harvesting - in a sterile and closely controlled environment.
The Z® RP control unit ensures safe operation of all cell- and tissue culturing processes conducted in Z® RP bioreactors and Z® RP GMP Breeders. System configurations and process parameters are easily accessible by the integrated touch screen. All relevant cultivation data are logged and evaluated on a personal computer with our implemented standard software according to GMP standards
一种新型的细胞及组织工程技术…Z®RP 全自动三维细胞及组织培养系统建立了一种独特的,快速可控的扩增贴壁细胞的技术平台。遵照GLP, GCP, GTP 和GMP标准,Z® RP全自动三维细胞及组织培养系统可让用户实现极高密度的细胞培养,并可诱导出类似于组织、可植入细胞外基质的细胞组织。
 
德国Zellwerk公司的Z®RP技术为再生医学领域开创了全新的视野:即作为扩增并获得原代细胞和干细胞的理想工具。治疗应用的范围也包括组织工程学,甚至于生产由植入支架衍生而来的完整的植入体。此外,在紧凑的Z®RP培养系统下,遵循成本效益用以生产重组的生物制药,疫苗,载体等。研究者们将从这种培养配置的变型中获益,从而极大加快了实验项目的发展。
 
Z® RP 培养系统由 Z®RP 生物反应器,Z®RP GMP工作仓和Z®RP 控制系统组成。 Z®RP GMP在繁育器无菌环境的调控下,多种类型的细胞组织培养过程都是可行的。
 
 
Z® RP 技术使得以下几方面更为先进:
安全快速的扩增贴壁细胞
在细胞特异性基质(圆盘形陶瓷培养载体)中进行三维的细胞高密度培养
扩增并获得可用于治疗的有活性的原代细胞
控制分化状态扩增干细胞
为细胞的植入扩增培养三维骨架
长期培养分泌细胞
高效生产重组蛋白和疫苗
生产优质的糖蛋白
 
Z ® RP 生物反应器
紧凑,灵活的培养且有益细胞…Z®RP生物反应器易于组装和使用。它们通常是在灌注模式下运行,以大量细胞聚集于极小空间的形式为主。中间装置是一个磁耦合旋转轴,与细胞组织载体装在一起,载体可有选择的使细胞暴露于介质,也可使其交替重叠。从高度多孔Sponceram®圆盘载体到植入支架,各种各样的支持物都可以安装在 Z®RP 生物反应器上,这就产生了多种多样的培养选择。在所有配置中尽可能最佳的给氧和供应养料是有保障的。温和的旋转运动使得细胞不会因为外加剪切力的影响而受惊,从而促进细胞及组织的粘附和快速增殖。细胞群体能保持活力并可表达大量的细胞外基质。三维高密度培养可以延长至数月,而生存力和表达产率不会降低。采用特殊的旋转程序结合使用分离溶液,收集获得贴壁细胞也很容易。
 
Z ® RP GMP 工作仓
良好的生产规范…Z®RP工作仓是一种符合GMP标准的带有流层功能的创新供应系统。无菌且温度可控的工作室保护 Z® RP 生物反应器免受污染,使用户可以进行所有必要的操作,例如细胞组织样品的制备,接种,介质容器的更换,管件的更换,传感器的更换,细胞收集等都可在无菌且严密可控的环境下进行。
 
Z ® RP 控制单元
可控且可记录的细胞组织培养…Z®RP控制单元确保在 Z®RP 生物反应器和 Z® RP GMP 工作仓指导下,所有细胞组织培养过程的安全运行。系统配置和过程参数易于通过集成触摸屏获得。所有相关的培养数据可被记录,并可经遵循 GMP 标准的标准执行软件在个人电脑上进行评估。
 
SPONCERAM ® 细胞培养载体
三维高密度细胞培养… Sponceram® 陶瓷载体是植入和扩增所有贴壁细胞的理想支持物。设计为高度多孔圆盘状的 Sponceram® 载体拥有极高的表面。Sponceram®的吸收特性诱导,甚至可在接种过程中诱导载体表面上细胞的分离。随后,锚固和扩增细胞进展快速,并导致最小化滞后阶段的出现。细胞培养在这种情况下,逐渐形成了形态,分化,长寿和生产力等方面的生理特性。
Sponceram® HA 支架是部分或全部可生物矿化的,代表着软骨细胞,成骨细胞和各种祖细胞的理想支持物。接种于 Sponceram® 载体上的产品分泌细胞株形成三维,像组织般层次稠密的细胞群。即使在多月缺乏蛋白质供给的情况下,大量的具有良好后处理模式样品的重组蛋白质也可被生产出来。

 

Microscopic image of “von Kossa” stained human umbilical cord stem cells at the end of cultivation and a blank value

左半部分是植入Sponceram®载体的人脐带干细胞在培养结束后,经Von Kossa染色后的显微照片;右半部分是空白Sponceram®载体染色对照

 

Microscopic image of “Alizarin red” stained human umbilical cord stem cells at the end of cultivation and a blank value

左半部分是植入Sponceram®载体的人脐带干细胞培养结束后,经Alizarin red染色后的显微照片;右半部分是空白Sponceram®载体染色对照

Microscopic image of umbilical cord stem cells, adhered on Sponceram® carrier discs shortly after seeding
植入Sponceram®载体培养的脐带干细胞粘附在该载体表面,经Giemsa染色后的显微照片

Microscopic image of adhered CHO cells (Giemsa stained) on a Sponceram® structure 24 h after seeding
植入Sponceram®载体培养24小时后的CHO细胞,经Giemsa染色后的显微照片