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MT-02-001-01,MT-02-001-04,CS-07-002,Rat liver microtissues formed by primary hepatocytes (96x),
MT-02-001-01,MT-02-001-04,CS-07-002,Rat liver microtissues formed by primary hepatocytes (96x),
  • MT-02-001-01,MT-02-001-04,CS-07-002,Rat liver microtissues formed by primary hepatocytes (96x),

MT-02-001-01,MT-02-001-04,CS-07-002,Rat liver microtissues formed by primary hepatocytes (96x),

产品报价:询价

更新时间:2019/4/2 12:09:54

地:美国

牌:insphero

号:MT-02-001-01,MT-02-0

厂商性质: 生产型,贸易型,服务型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

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3D InSight™ Rat Liver Microtissues for in-vitro liver toxicology

Delivered assay-ready for you to use

  • The only assay-ready co-culture model of primary hepatocytes with NPCs
  • Immuno-competent Kupffer-macrophages incorporated
  • Suitable for idiosyncratic toxicity and chronic toxicity with repeated dosing

 

Organotypic cell composition
3D rat-liver microtissues are generated by co-culture of primary rat hepatocytes and non-parenchymal cells (NPCs) to reflect the organotypic cell composition. Liver-derived endothelial cells surround the hepatocytes, building a physiological barrier between cell-culture medium and hepatocytes. Kupffer macrophages are incorporated between hepatocytes and are sensitive to external stimuli like bacteria-derived lipopolysaccharide (LPS). No bioartificial scaffold materials are used.

3D rat-liver microtissues: Delivered in Gravity-TRAP™ plates – ready for you to use

3D InSight Rat Liver Microtissues enable long-term toxicology in-vitro

 

Superior functionality
InSphero’s unique scaffold-free production technology assures an abundance of tight cell-cell contacts. As a result, the hepatocytes remain polarized, differentiated and active. With a viability of up to five weeks in culture, chronic toxicity testing at repeat dosing is finally possible. An intact bile-canaliculi network ensures proper clearance of bile-acids into the medium. This model is even capable of de-novo synthesis of bile acids.

Histology of a 3D rat liver microtissue spheroid with a bile-canaliculi marker in green Immunofluorescence staining with ICAM-1 (red) and DPPIV (green). DAPI is shown in blue. Provided by Seddik Hammad and Prof. Jan G. Hengstler, IfaDo Dortmund, Germany

 

Inflammation-mediated toxicity
Hepatocytes alone are often not sufficient to understand in-vivo toxicology. For instance, cases of idiosyncratic toxicity are often mediated by inflammation involving Kupffer macrophages. Because InSphero’s 3D cell culture technology allows for co-culture of different cell types, the 3D rat-liver microtissue contains viable and competent Kupffer cells, which enables to reproduce inflammation- mediated toxicity in vitro.

An exposure of the rat-liver microtissues shows inflammation-mediated, idiosyncratix toxicity A co-exposure of the rat-liver microtissues with the withdrawn drug Trovafloxacin (top) and an inflammagen (LPS) is identified as toxic, while the safe Levofloxacin (bottom) shows no toxicity.

 

Supplied to you ready-to-use
3D rat liver microtissues are supplied ready-to-use in the specially designed GravityTRAP™ plate. In this format automated plate readers can easily locate and analyse microtissues. In addition, the plate is designed to facilitate media changes and compound dosing during long term maintenance. The GravityTRAP™ plate is also suitable for standard luminescent and fluorescent assays, e.g. Promega ATP, LDH, GSH and apoptosis assays.

 

Ordering information

Catalog # Description
MT-02-001-01 Rat liver microtissues formed by primary hepatocytes (96x)
MT-02-001-04 Rat liver microtissues from primary hepatocytes, co-culture with nonparenchymal cells (96x)
CS-07-002 3D microtissue maintenance medium, for rat liver (500 mL)

Production runs every two weeks, special formulations upon request.