来宝网Logo

热门词:生物显微镜 水质分析仪 微波消解 荧光定量PCR 电化学工作站 生物安全柜

AED
现在位置首页>行业专用>生命科学仪器>DNA测序仪/基因分析仪>Light Scanner 快速基因突变扫描和基因分型的高通量检测系统
Light Scanner 快速基因突变扫描和基因分型的高通量检测系统
Light Scanner 快速基因突变扫描和基因分型的高通量检测系统
  • Light Scanner 快速基因突变扫描和基因分型的高通量检测系统

Light Scanner 快速基因突变扫描和基因分型的高通量检测系统

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:Biofire

号:Light Scanner

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

1237
访问人数
0
累计评论


王荣 : (18618101725) (18618101725)

(联系我时,请说明是在来宝网上看到的,谢谢!)


美国Biofire Diagnostics介绍:
美国Idaho公司是致力于研发生命科学领域中用于微生物鉴定和核酸分析的快速高效的分析仪器的高科技公司,

产品包括:

1、DNA扩增的RapidCycler PCR,

2、实时荧光定量PCR LightScanner 32,

3、高分辨率熔解曲线分析LightScanner96/384及HR-1,

3、突变检测和基因分型等。

Idaho公司在Mutation Discovery和Genotyping科研领域中具有世界顶尖科技的产品。此外,在水及食品安全检测领域具有领先的产品可科技。

Light Scanner 高分辨溶解曲线基因突变/基因分型检测系统是美国Idaho公司经过7年的潜心研发而商品化的世界上第

一台专门用于快速基因突变扫描和基因分型的高通量检测系统,5分钟即可完成96/384孔板样品突变检测,

尤其用于测序前的大规模突变筛查及基因分型工作,使测序的工作量降低为原来的1%。

此外LightScanner还可以检测到体细胞样品中比例低至0.1%的突变,特别适用于Cancer及流行病学大规模筛查。

美国Idaho公司高分辨基因突变扫描和基因分型分析系统.

LightScanner是第一台利用高分辨熔解曲线进行高通量(96或384孔PCR板系统)的基因突变扫描和基因分型分析的仪器。它具有极好的敏感性和特异性,其优势充分体现在温度控制和数据获取率。

适合高通量样品筛查项目。只需在PCR反应上加入LCGreen染色剂,样品经PCR扩增后将PCR板转放入LightScanner仪器中进行熔解曲线分析。


2012年Idaho Technology公司更名为BioFire Diagnostics

世联博研北京科技有限公司,是biofiredx中国区域总代理,签约代理:

1、Light Scanner 高分辨溶解曲线基因突变/基因分型检测系统

2、LightScanner 32 荧光定量PCR+HRM系统

3、DNA扩增的RapidCycler PCR,

4、实时荧光定量PCR LightScanner 32,

5、高分辨率熔解曲线分析LightScanner96/384及HR-1,

6、突变检测和基因分型等。

欢迎致电世联博研全国免费客服热线400-650-8506咨询、索取产品资料。

----------------------------------------------------------------------------------------------------------------------------------------------------------

高分辨率溶解曲线(高分辨溶解曲线突变检测)是理想的SNP检测手段:
快速、高通量—LightScanner 5分钟完成96/384孔板的PCR产物检测, 20000个SNP/天
准确、灵敏度高、特异性好—LightScanner100%准确性、特异性(<400bp);准确性96.4%和特异性99.1%(400~1kb)
重复性好—LightScanner重复性100%
费用低—LightScanner检测SNP每个样品的成本为1RMB
操作简便—LightScanner只需将PCR产物(96/384孔板)放入仪器内便可,软件自动基因分型

  • 仪器介绍

LightScanner® System

Mutation Discovery, Gene Scanning and Genotyping


The LightScanner is the first system to use Hi-Res Melting® (also known as High Resolution Melting, or HRM) analysis for mutation scanning and genotyping in 96- or 384-well plate system. Standardization is important for research, making the plate format preferable in many settings. It is compatible with nearly all thermal cyclers and qPCR machines.

The LightScanner is unsurpassed in its temperature control for plate-based systems. It also has the highest data quality. With superb sensitivity and specificity, this instrument is designed to meet the needs of high-throughput scanning projects. Simply add LCGreen® Plus dye to the initial PCR reaction– then transfer the PCR plate to the LightScanner for melting. Intuitive analysis software groups the results and

Benefits from a Complete Product Solution

Instrument

 

Chemistry

  Results in less than five minutes   Optimized Master Mix for superior PCR performance for Hi-Res Melting applications
  Hi-Res Melting is non-destructive allowing samples to be recovered for further analysis

Support

Software

  Obtain quick assistance through phone and online help desks   Software automatically analyses each sample and defines the standards and variants
  Work directly with highly experienced scientists   Simple data management tools
  Primer design software
 

LightScanner High Resolution Melting Analysis SoftwareLightScanner Software
 

Our comprehensive software package provides both assay design and data analysis modules. The primer design module streamlines assay design for Hi-Res Melting. The analysis software features Call-IT®, a patented scientific method where the software critically analyses each sample, defines and groups wild-types and variants. This software also provides users with simple data management tools.

 

 

High Resolution Melting References

The LightScanner Hi-Res Melting system has been used for over five years and has resulted in approximately 200 peer-reviewed publications, making it the most highly published Hi-Res Melting instrument. Here are a few select references for your review.

  1. De Koeyer D, Douglass K, Murphy A, Whitney S, Nolan L, Song Y, De Jong W (2010). Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato. Mol Breeding. 25, 67–90.

  2. Rolf H.A.M. Vossen, Emmelien Aten, Anja Roos, Johan T. den Dunnen. (2009). High-Resolution Melting Analysis (HRMA) - More than just sequence variant screening Human Mut. 30, 860-866.

  3. Parant JM, George SA, Pryor R, Wittwer CT, Yost HJ. (2009). A rapid and efficient method of genotyping zebrafish mutants. Dev Dyn. 238,3168-74.

  4. van der Stoep N, van Paridon CDM, Janssens T, Krenkova P, Stambergova A, Macek M, Matthijs G, Bakker E. (2009). Diagnostic guidelines for high-resolution melting curve (HRM) analysis: An interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. Human Mut. 30, 899-909.

  5. Aten E, White SJ, Kalf ME, Vossen RH, Thygesen HH, Ruivenkamp CA, Kriek M, Breuning MH, den Dunnen JT.(2008). Methods to detect CNVs in the human genome. Cytogenet Genome Res. 123, 313-21.

  6. Audrezet MP, Dabricot A, Le Marechal C, Ferec C. (2008). Validation of high-resolution DNA melting analysis for mutation scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. J Mol Diagn. 10, 424-34.

  7. C. Voegele, S.V. Tavtigian, D. de Silva, Cuber Thomas, F. Le Calvez-Kelm. (2007). A Laboratory Information Management System (LIMS) for a high throughput genetic platform aimed at candidate gene mutation screening. Bioinformatics. 23, 2504-2506.

LightScanner and Computer Specifications

Instrument Specifications
Size (w x d x h) 7.8” (20 cm) x 16.5” (42 cm) x 31.8” (81 cm)
Weight 25 kg / 55 lbs
Voltage Input 100-240 VAC, +/-10%, 50-60 Hz with automatic detection and configuration, 2.5 A
Wattage 800 W maximum including computer and monitor.
Cooling time 95° to 60 °C (non-linear)
~ 180 seconds at ambient temperature 25 °C
Heating rate 0.1 °C per second
Single run time 15 minutes, including cool down
Certifications CE, UL (for Electrical Safety)

Sample Handling and Filter System
Sample format Standard 96- or 384-well trays
Reaction volume 5-100 µl
Sample temperature range 40° to 100 °C
Excitation filter 470 +/- 20 nm
Emission filters 510 and 600 nm

Computer and Software
LightScanner Software Accurately performs mutation scanning and genotyping by Hi-Res Melting curve analysis
Computer Pentium-based PC with Windows XP Professional, 17”- 1280 x 1024 graphics monitor and software.
Networking Ethernet connection allows export of data in standard spreadsheet format compatible with LIMS software.
 

Ordering Information
LightScanner System
Includes instrument, computer, software and Start-up Kit
 
96-Well Instrument
384-Well Instrument
LSCN-ASY-0011
LSCN-ASY-0001