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compucyte Enhanced Blue Cube
compucyte Enhanced Blue Cube
  • compucyte Enhanced Blue Cube

compucyte Enhanced Blue Cube

产品报价:询价

更新时间:2023/4/3 18:11:18

地:美国

牌:compucyte

号:250-1207-000

厂商性质: 生产型,

公司名称: 世联博研(北京)科技有限公司

产品关键词:

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激光扫描共焦显微镜(laser scanning confocal microscope,LSCM),也被称为激光扫描细胞仪(laser scanning cytometer,LSC)。自20世纪70年代问世以来很快得到迅速发展,成为分子细胞生物学的新一代研究工具。激光扫描共焦显微镜在显微镜基础上配 置激光光源,扫描装置,共扼聚焦装置和检测系统,整套仪器均由计算机自动控制,专用软件监控和执行各组件之间的切换。生物医学应用中LSCM的显微镜以倒置显微镜为主,便于活细胞检测。与普通光镜和荧光显微镜相比有一些明显的优点,其中主要有:

Enhanced Blue Cube

The Enhanced Blue Cube allows scans with the 405nm (Violet Diode) and 488nm (Argon) lasers to be performed in a single pass, halving the scan time required for assays using both Violet and Argon laser-excited dyes.

 

详请访问:http://www.compucyte.com/Enhanced%20Blue%20Cube-RevB-3.pdf

 

 

美国CompuCyte Corporation 是一家未公开上市公司,总部位于马萨诸塞州剑桥。该公司是开发用于生命科学研究、药物安全、毒物学研究以及临床试验的细胞分析仪器的领导者。该公司的 iCyte(R)、iCys(R) 和 iColor(TM) 血细胞计数器使细胞与组织样本的定量流式图像分析成为可能。

CompuCyte develops Laser Scanning Cytometry products for cell-based and tissue analysis, using advances in optics, computer technology, and imaging techniques. CompuCyte’s products provide life scientists, pharmaceutical researchers, and clinical laboratory professionals with unique high-information content analysis and reporting capabilities for discovery and objective characterization of the biophysical and biochemical cellular processes that define the normal and disease states.

Founded by Dr. Louis Kamentsky, internationally recognized for his pioneering work in the field of flow cytometry, CompuCyte’s Laser Scanning Cytometry provides life science researchers with the ability to undertake high-content analysis of cell and tissue specimens on a solid substrate, not feasible with any other product.

CompuCyte ’s platform technology, Laser Scanning Cytometry, has applications in basic life science research, drug discovery, and molecular pathology. The Company’s products based on this technology include the iCyte™ Automated Imaging Cytometer, the iCys™ Research Imaging Cytometer, the LSC® Laser Scanning Cytometer, and our revolutionary new development, the iColor™ Fluoro-Chromatic Imaging Cytometer. When combined with iBrowser™ Data Integration Software and the iNovator Application Development Toolkit, CompuCyte’s family of instruments provide unmatched flexibility in specimen formats and automation options, combined with analytical performance (quantitative cell-based analysis) and specimen visualization.

iCyte, using an inverted microscope platform, automates higher-volume, high-content cellular analysis performed on a variety of carriers, including microtiter plates and microscope slides. When combined with the optional robotic specimen loader, iCyte allows full walk-away analysis in areas of research that call for processing large numbers of samples, such as drug toxicity or biomarker discovery often performed on multiple tissue slides or with tissue microarrays.
iCys allows specimen visualization through its fully-configured microscope along with both laser scanning and brightfield visualization capabilities. iCys is quickly becoming the analytic tool of choice for life science research.
iColor combines the advantages of flow cytometry, fluorescence image analysis, and immunohistochemistry in one unique system. iColor allows simultaneous analysis of both fluorescent and chromatic staining in the same specimens, opening new applications to the power of quantitative analysis.
The Company’s prestigious customer base includes the top scientific and clinical research institutions (both governmental and universities), leading biotechnology and pharmaceutical companies, and the most highly regarded hospitals and reference laboratories worldwide.

(1)普通显微镜使用的光源为卤素灯,光谱范围宽,成象时样品上每个照光点均
会受到色差影响以及由照射光引起的散射和衍射的干扰,影响成象质量。LSCM的光源为激光,是单色性好的平行光,基本消色差,成象聚焦后焦深 小,纵向分辨率高,可无损伤地对样品作不同深度的层扫描和荧光强度测量。
(2)普通荧光显微镜分辨率低,显示的图象结构为多层面的图象迭加,结构不够
清晰。LSCM结构上采用双针孔(pinhole)装置,形成物象共扼的独特设计(如图2-2所示)。激光通过聚光镜焦平面上针孔形成点光源经 物镜在焦平面上对样品进行逐点扫描,样品上每个照射点,反射后经过物镜折射到像焦平面的探测针孔处成像,经空间滤波后,有效地抑制同焦平面上非测量光点形 成的杂散荧光和样品的不同焦平面发射来的干扰荧光。每个像点被光电倍增管(PMT)或冷电感藕合器件(cCCD)探测器接收。因为光学系统物象共扼,只有物镜焦平面上的点经 针孔空间滤波才能形成光点图象,扫描后可得到信噪比极高的光学横断面,分辨率比普通光学显微镜提高1.4倍。LSCM能以0.1um的步距沿轴向对细胞进 行分层扫描,得到一组光学切片,不同焦平面的光学切片经三维重建后能得到样品的三维立体结构,这种功能被形象地称为"显微CT"。
(3)LSCM的高灵敏度、高分辨率和高放大倍数,减少了光淬灭的影响,提供了
普通光学显微镜无法显示的结构信息,并适用于达到毫秒级的快速变化检测。
LSCM最常用的功能是荧光检测、三维重建和显微操作等。其中荧光检测覆盖的内容极为广泛,通过多种荧光探针或荧光连接抗体,可对细胞内离子、 pH值、各种蛋白质分子进行动态测定。另外利用激光扫描还可以对细胞进行特殊操作,例如光刀切割法(cookie-cutter) 作粘附细胞分选(adhered cell sorting),能杀灭不需要的细胞,保留所选细胞亚群继续培养;激光光陷阱技术(又称为光镊技术)对目标细胞进行非接触式的捕获和固定,并进行精确操 作;激光作为光子刀可以用来完成细胞膜瞬间打孔以及对线粒体、溶酶体、染色体和神经元突起的切割等显微细胞外科手术。