美国flexcell细胞组织流体切应力系统

简介：

flexcell str-4000，细胞流体切应力系统，为细胞提供各种流体应切力：稳流式切应力、脉冲式切应力、往返式切应力等，

是当今科研界最先进细胞流体切应力系统

Flexcell® Streamer® Fluid Shear Stress Device流体切应力系统
• 为细胞提供各种形式的流体切应力：稳流式切应力，脉冲式切应力或者往返式切应力。
• 在经过特殊基质蛋白包被的25mm x 75mm x 1.0mm 细胞培养载片上培养细胞。
• 计算机控制的蠕动泵可以调节切应力的大小，从0-35 dynes/cm.
•  
• 通过Osci-Flow液体控制仪提供往返式或者脉冲式的流体切应力。
• 检测细胞在液流作用下的排列反应。
• 设备易拆卸并可高温消毒。
• 可以在经过特殊包被的6个细胞培养载片上同时培养细胞。
• 提供两个液流脉冲阻尼器。
• 
  Streamer System产品包括：
• Streamer设备
• DELL笔记本式计算机
• 快拆接头及胶管
• 蠕动泵
• StreamSoft软件i>
• 2个液流脉冲阻尼器
• 12个细胞培养载片(Culture Slip)

产品详细:

International Corporation Flexcell® Fluid Shear Systems

Fluid Shear System
Fluid-induced shear stress occurs in every tissue in the body as a result of interstitial fluid movement. Tissue deformation by compression, tension or shear forces results in the movement of interstitial fluid around cells. Fluid movement acts as a transport vehicle for ions, proteins, carbohydrates and other molecules capable of movement within the matrix. As the fluid moves past cell membranes, a shear stress, t, is generated. If one assumes that laminar flow occurs through a parallel-plate flow chamber, fluid-induced shear stress values can be determined with the following formula: t = 6uQ/bh², where t is the shear stress in dyne/cm², u is the viscosity of the fluid in dynes/cm², Q is the flow rate in ml/s, b is the width of the flow channel in cm, and h is the height of the flow channel in cm.

The Streamer® is a parallel-plate flow system that is used to apply fluid-induced shear stress to cells grown in a monolayer. The system includes a six-chamber laminar flow device designed to hold 75 x 25 x 1 mm Culture Slips®. The Streamer® system can be used to apply laminar, pulsatile*, or oscillating* flow to cell in monolayer culture (Figure 3) and uses a computer-controlled peristaltic pump to regulate shear stress from 0-35 dynes/cm².

*When used in conjunction with the Osci-Flow® Flow Controller.

Flexcell® Osci-Flow® Flow Controller
 

A flow control system that gives the user the ability to regulate the frequency of oscillation or pulsatile patterns as well as shear stress levels applied to cells through StreamSoft™ and NI-DAQ software.

• Reverses fluid flow instantaneously.
• Reduces wear and tear on pumps and motors.
• Adapts to other perfusion systems.

The Flexcell® FlexFlow™ Shear Stress Device is a parallel plate laminar flow device designed to apply fluid shear stress and/or cyclic strain to cells in culture while providing a means for viewing cell activity under a microscope in real time. Like the StageFlexer®, the FlexFlow™ fits on the stage of a standard upright microscope.

For fluid flow on the micro and nanofluidic levels, Flexcell has the HiQ Flowmate Fluid Control System. The HiQ Flowmate® unit is a dual syringe pump that utilizes five separate modules: constant, continuous, ramped, oscillatory, and pulsatile flow to represent a variety of applications. In addition to applying fluid shear stress to cells in culture, this device can also be used for diluting, dispensing, & injecting; animal drug infusion and fluid withdrawal; mixing & proportioning fluids, among other applications.

FlexFlow™The FlexFlow™ is a parallel plate laminar flow device designed to apply fluid shear stress and/or cyclic strain to cells in culture while providing a means for viewing cell activity under a microscope in real time. The FlexFlow™ fits on the stage of a standard upright microscope.

Osci‐Flow®The Osci-Flow®

Osci‐Flow®The Osci-Flow® is a software-controlled valve-operated device designed to provide oscillatory or start-stop flow patterns to cells cultured in the Streamer®, FlexFlow™ or other perfusion devices. The Osci-Flow® device is able to regulate flow both in an oscillatory (flow reversal) manner and in a pulsatile (square wave) mode, where the flow is stopped and then restarted after a designated period of time. The StreamSoft™ software allows the user to regulate frequency of oscillation or pulsatile patterns, as well as shear stress levels applied to cells.

Why fluid shear stress

Fluid-induced shear stress occurs in every tissue in the body as a result of interstitial fluid movement. Tissue deformation by compression, tension or shear forces results in the movement of interstitial fluid around cells. Fluid movement acts as a transport vehicle for ions, proteins, carbohydrates and other molecules capable of movement within the matrix. As the fluid moves past cell membranes, a shear stress, τ, is generated. Applying stresses to cells in culture simulates the in vivo environment causing dramatic morphologic and biochemical responses.

Pathways activated in response to applied mechanical load figure：

Apply fluid shear to cell swith laminar,pulsatile,oroscillating flow：

Culture Slips® Microscope Slides and Coverslips

 

• Culture Slips® are available in two sizes: 75 mm x 25 mm x 1.0 mm (for Streamer® or FlexFlow™), 75 mm x 24 mm x 0.2 mm (for FlexFlow™ only).
• The 25 mm x 75 mm x 1.0 mm Culture Slips® are rimmed with a 1.0 mm wide Teflon® border to help limit cell culture growth to the portion of the slip exposed to fluid flow.
• Available with covalently bound extra cellular matrix surfaces such as Collagen Type I, Elastin, Pronectin®, Laminin, Amino, as well as untreated.

StageFlexer® Membranes for the FlexFlow™

 

Flexible growth surfaces that are individually packaged free membranes for the StageFlexer® or FlexFlow™ dynamic culture products.

• Applications consistent with BioFlex® culture plates.
• Membrane is clear for direct viewing of cells.
• Membranes are in a closed SpillGuard® culture dish to maintain sterility even after outer packaging is opened.

美国Flexcellint国际公司，成立于1987年，该公司专注于细胞力学产品的设计和制造。以提供独特的体外细胞拉伸、压缩和剪切应力仪器设备以及配套的培养板、硅胶膜载片等耗材闻名于世。

世联博研将作为flexcell公司中国大陆独家代理商，为全国用户提供flexcell全系列产品， 详情致电全国免费客服电话：400-650-8506咨询索取资料。

更多关于世联博研介绍请访问公司网站：http://www.bio-goods.com

信箱：SLBY800@163.COM

联系人：王经理

电话：010-67529703，手机：13466675923、18618101725、13261877206

一、仪器部分:

• 1、FX-5000C细胞压力加载系统(flexcell FX5000 Compression system)

该系统对各种组织、三维细胞培养物提供压力加载，并能通过独特显微设备实时观察细胞在压力作用的反应。

• 2、FX-5000T细胞应力加载系统(Flexcell FX5000 Tension system)

该系统对二维细胞和三维细胞提供双轴向应力或单轴向应力加载，并能通过独特显微设备实时观察细胞在应力作用的反应。

• 3、Flex Jr. Tension显微应力加载系统(Flex Jr. Tension)

该系统对显微镜下的细胞提供双轴向应力或单轴向应力加载。

• 4、TissueTrain三维细胞组织培养系统(Flexcell Tissue® Train System)

该系统对生成在三维状态下的细胞进行静态或者周期性的应力刺激加载分析，并能通过独特显微设备实时观察细胞在机械力刺激下的反应。

• 5、STR-4000细胞流体切应力系统(Flexcell Fluid Shear Stress Device)

该系统为细胞提供各种形式的流体切应力：稳流式切应力，脉冲式切应力或者往返式切应力，并能通过独特显微设备实时观察细胞在机械力刺激下的反应。

• 6、应力/压力传导仪(FlexLink® FX-5000TFL/FX-5000CFL Tension or Compression)

• 7、flexcell自动扫描设备和XyFlEX计算软件(ScanFlex with XyFlex)

该系统记录三维人工组织中凝胶压实的过程自动、重复扫描。分辨率可达600pdi；可以设备扫描频率、次数；与FX-5000TT一起使用，可以记录三维细胞培养凝胶的压实动力学。存储的图像可以输入XyFlEX®程序进行凝胶面积的计算。

• 8、HiQFlowmate液体控系统(HIQ-4000-A/B/C HiQ Flowmate-Fluid Control System)

该系统双注射泵可以在微升、纳升、微微升水平上控制液体流。

• 9、OsciFlow_切应力模式控制器

• 10、SFlex_SFlexJr_显微应力加载系一号(StageFlexer StageFlexer Jr. Microscopy Tension Device)

• 11、FlexFlow显微切应力加载设备二号(StageFlexer StageFlexer Microscopy Tension Device)

• 12、STR-4000C 流体剪切系统W/O型电脑(Streamer Fluid Shear System w/o Computer)

• 13、STR-4000D Streamer Fluid Shear Body Only

• 14、Flexcell 储压泵(Pressure Reservoir)

• 15、FlexStop真空隔离阀

• 二、flexcell培养耗材部分:
• 1、TTPlate三维细胞培养板
• 2、BioFlex（flexcell BioFlex培养板）
• 5、UniFlex（flexcell双向应力培养板）
• 6、BioPress压力加载系统配套使用的BioPress培养板
• 7、细胞培养载片
• 8、圆形TTCircular三维细胞培养板
• 9、HI bioFlex培养板
• 10、SFMembrane_硅胶膜

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