Abcam ab1386 Mouse monoclonal [2D2] to Bax prediluted 6ml
钱先生 : (18621872635) (4000210588)
(联系我时,请说明是在来宝网上看到的,谢谢!)钱先生 : (18621872635) (4000210588)
(联系我时,请说明是在来宝网上看到的,谢谢!)Anti-Bax antibody [2D2], prediluted
参阅全部 Bax 抗体 (25) ...
Mouse monoclonal [2D2] to Bax, prediluted
This antibody recognizes a 211 kDa protein identified as bax protein. This antibody is highly specific to bax and does not cross react with bcl-2 alpha or bcl-X protein.
IHC-P, IHC-Frmore details
Reacts with
Human
Does not react with
Mouse, Rat
BALB/C mice were injected with a synthetic peptide amino acids 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
C-GSGEQPRGGGPTSS
Liquid
Store at +4°C. Do not freeze.
Preservative: 0.05% Sodium Azide
Constituents: Inert stabilizer
Monoclonal
2D2
IgG1
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Our Abpromise guarantee covers the use of ab1386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA, pH 8.0 prior to immunostaining.
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Belongs to the Bcl-2 family.
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.