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现在位置首页>实验试剂制品>抗体>抗体>Abcam ab13858 Rabbit polyclonal to MDC1 200ul
Abcam ab13858 Rabbit polyclonal to MDC1 200ul
Abcam ab13858 Rabbit polyclonal to MDC1 200ul
  • Abcam ab13858 Rabbit polyclonal to MDC1 200ul

Abcam ab13858 Rabbit polyclonal to MDC1 200ul

产品报价:询价

更新时间:2013/6/20 13:23:01

地:美国

牌:Abcam

号: ab13858

厂商性质: 生产型,贸易型,服务型,

公司名称: 上海盈东生物科技有限公司

产品关键词:

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Overview

Product name

Anti-MDC1 antibody
参阅全部 MDC1 抗体 (11) ...

Description

Rabbit polyclonal to MDC1

Tested applications

WBmore details

Cross reactivity

 

Reacts with

Human

Does not react with

Mouse, Rat

 

Immunogen

Synthetic peptide: RQESDKNGESASPERDRE , corresponding to amino acids 871-888 of Human MDC1.

RQESDKNGESASPERDRE

Positive control

Jurkat whole cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS

Concentration

 

  • 200 µl

 

Purity

Protein G purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
 

  • Datasheet PDF
  • Western blot - MDC1 antibody (ab13858)Western blot - MDC1 antibody (ab13858) image(enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

    WB: 1/200 - 1/1000. Dete...Read more →

    WB: 1/200 - 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 250 kDa).

Target

Function

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

Tissue specificity

Highly expressed in testis.

Sequence similarities

Contains 2 BRCT domains.
Contains 1 FHA domain.

Domain

Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.

Post-translational
modifications

Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.

Cellular localization

Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.

Information by UniProt

Alternative names

  • DKFZp781A0122 antibody
  • Homologue to Drosophila photoreceptor protein calphotin antibody
  • KIAA0170 antibody
see all

Database links

  • Entrez Gene: 9656 Human
  • GenBank: NP_055456 Human
  • Omim: 607593 Human
  • SwissProt: Q14676 Human
  • Unigene: 433653 Human
  • Unigene: 653495 Human

Anti-MDC1 antibody images:

Western blot - MDC1 antibody (ab13858)

Western blot - MDC1 antibody (ab13858)

 

 



Predicted band size : 250 kDa


Western blot analysis of MDC1 in 30 ugs of Jurkat cell lysate using ab 13858 at 1/1000 dilution.