参阅全部 MDC1 抗体 (11) ...
Rabbit polyclonal to MDC1
Does not react with
Synthetic peptide: RQESDKNGESASPERDRE , corresponding to amino acids 871-888 of Human MDC1.
Jurkat whole cell lysate
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Protein G purified
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Western blot - MDC1 antibody (ab13858)(enlarge)
Our Abpromise guarantee covers the use of ab13858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 250 kDa).
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Highly expressed in testis.
Contains 2 BRCT domains.
Contains 1 FHA domain.
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
Target information above from: UniProt accessionQ14676The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MDC1 antibody (ab13858)
Predicted band size : 250 kDa
Western blot analysis of MDC1 in 30 ugs of Jurkat cell lysate using ab 13858 at 1/1000 dilution.