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现在位置首页>实验试剂制品>抗体>抗体>Abcam ab13854 Rabbit polyclonal to CXCR4 200ul
Abcam ab13854 Rabbit polyclonal to CXCR4 200ul
Abcam ab13854 Rabbit polyclonal to CXCR4 200ul
  • Abcam ab13854 Rabbit polyclonal to CXCR4 200ul

Abcam ab13854 Rabbit polyclonal to CXCR4 200ul

产品报价:询价

更新时间:2013/6/20 13:23:01

地:美国

牌:Abcam

号: ab13854

厂商性质: 生产型,贸易型,服务型,

公司名称: 上海盈东生物科技有限公司

产品关键词:

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Overview

Product name

Anti-CXCR4 antibody
参阅全部 CXCR4 抗体 (19) ...

Description

Rabbit polyclonal to CXCR4

Tested applications

WBmore details

Cross reactivity

 

Reacts with

Mouse, Rat, Human

 

Immunogen

Synthetic peptide: ILSKGKRGGHS, corresponding to amino acids 328-338 of Human CXCR4.

ILSKGKRGGHS

Positive control

Whole cell lysate from HeLa cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS

Concentration

 

  • 200 µl at 0.5mg/ml

 

Purity

Protein G purified

Purification notes

This antibody is affinity purified.

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
 

  • Datasheet PDF
  • Western blot - CXCR4 antibody (ab13854)Western blot - CXCR4 antibody (ab13854) image(enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

    WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

Target

Function

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.

Tissue specificity

Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.

Involvement in disease

Defects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Domain

The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.

Post-translational
modifications

Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.

Cellular localization

Cell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.

Information by UniProt

Alternative names

  • C-X-C chemokine receptor type 4 antibody
  • CD184 antibody
  • CD184 antigen antibody
see all

Database links

  • Entrez Gene: 7852 Human
  • Entrez Gene: 12767 Mouse
  • Entrez Gene: 60628 Rat
  • Omim: 162643 Human
  • SwissProt: P61073 Human
  • SwissProt: P70658 Mouse
  • SwissProt: O08565 Rat
  • Unigene: 593413 Human
see all

Anti-CXCR4 antibody images:

Western blot - CXCR4 antibody (ab13854)

Western blot - CXCR4 antibody (ab13854)

 

 



Predicted band size : 42 kDa


Western blot analysis of 15 µg of whole cell lysate from HeLa cells with ab13854 at 1 µg/ml detecting CXCR4.