Mouse Bioarrays

CodeLink Mouse Whole Genome Bioarray
The Mouse Whole Genome Bioarray offers comprehensive genome-wide gene expression analysis on a single bioarray.

CodeLink Mouse Focused Bioarrays

CodeLink Mouse Focused Bioarrays provide the researcher with subsets of the whole genome array to enable pathway and process specific research.

The arrays are built in a 16-assay format, allowing for lower sample volume and higher assay throughput.

Applied Microarrays will partner with you to create the required subset of interest.

In addition, we offer catalog Inflammation and ADME subsets which have been compiled in collaboration with expert researchers from that field

Fig. CodeLink Mouse Whole Genome Bioarrays exhibit excellent array- to-array signal intensity reproducibility. The plotted normalized probe signals from two independent CodeLink Mouse Whole Genome Bioarrays-each run with 10 µg of the same labeled brain cRNA target-are shown here. Between these two bioarrays, 99.6% of all probes with "good" signals were within two-fold of each other. The blue data points represent the concordantly "good" probes; the red data points represent the concordantly "absent" probes. The two-fold lines are shown in gray.

Fig. CodeLink Mouse Whole Genome Bioarrays exhibit excellent reproducibility within and across bioarray production batches as represented by median % CV. These data were generated using one brain cRNA sample on three replicate arrays from one production batch (within batch, blue), and one array from each of three production batches (across batch, red).

Specifications

Mice exhibit high similarity to many human biological pathways, making it a key model system for human physiological and toxicological studies in drug discovery applications. The probe sequences, representing well annotated, full length and partial mouse gene sequences, were designed based on sequences selected from the NCBI UniGene build #139, RefSeq database (April 1, 2004 release) and dbEST database (July 31, 2004 release).Well-annotated mRNA or coding sequences were chosen to ensure usefulness for a large range of applications in basic research, biotechnology, and drug development. Each sequence was carefully screened to ensure high-quality, specific probe design, and to reduce redundancy of gene targets. All of the probes designed to these sequences were functionally tested, and over 97% were functionally validated against 14 mouse tissues to ensure best representation of the gene and biologically relevant results.

product specifications

 

expression bioarrays

 

CodeLink Mouse Whole Genome Bioarray

 

CodeLink™ Mouse Whole Genome Bioarray has 34,967 discovery probes representing up to 36,142 unique transcripts. The transcript sequences were selected from the NCBI UniGene build #139, the RefSeq database (April 1, 2004 release), and the dbEST database (July 31, 2004 release). In addition to the discovery probe sequences, each bioarray contains positive and negative bacterial control probes for monitoring array performance. All oligonucleotide probes are 30 bases long.

Probe sets	Probes/gene	Redundancy	Dispense count
Number of mouse probes	34,967 unique sequences	1	34,967 probes
Positive bacterial control probes	5 probes/gene × 6 bacterial targets	30	300 probes
Negative bacterial control probes	32 negative control probes	32	320 probes
TOTAL			35,587 probes

Performance specifications

 

The following data are based on results from the final product verification studies conducted using a series of bioarrays and targets generated from mouse tissue.

Performance parameter	Performance requirement	Verified assay performance
Specificity	Discrimination > 2 base mismatches	Discrimination > 2 base mismatches
Sensitivity	1:1,024,000 mass ratio (bacterial spike)*	1:2,048,000 mass ratio (bacterial spike) with cRNA spiking at the cRNA level*, **
Dynamic range	Linear signal response over two orders of magnitude	Linear signal response over three orders of magnitude; 0.05 pM – 50 pM
Signal reproducibility (total CV)	< 20% total median CV	12.2% total median CV across production batches 7.3% median CV for all spots excluding spots below noise
Minimum detectable fold change	95% within two‐fold change for the same tissue	98.2% within two‐fold change for the same tissue for all good spots across different production batches
Input sample	0.1 µg – 2 µg total RNA	0.1 – 2.0 µg total RNA***

* mass ratio spiking of cRNA control spike into cRNA target

** the lowest level tested in verification experiments with cRNA spiking

*** generates sufficient amplified material for 1‐8 replicates depending on tissue and starting amount

Storage and handling conditions

 

Upon receipt, store CodeLink™ Mouse Whole Genome Bioarrays with desiccant at room temperature.

Safety precautions

 

 

Standard laboratory safety procedures should be followed when using this product. Safety glasses, a lab coat, and appropriate gloves should be worn at all times when in the laboratory.