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现在位置首页>实验试剂制品>抗体>抗体>Abcam ab10530 Mouse monoclonal [FC82291] to Nucleophosmin 100ul

Abcam ab10530 Mouse monoclonal [FC82291] to Nucleophosmin 100ul

产品报价:¥询价

更新时间:2013/6/20 13:23:01访问人数:48 次

地:上海 上海

厂商性质:生产商牌:

公司名称: 上海盈东生物科技有限公司号:ab10530

产品关键词:

联系人:钱先生 (18621872635) (4000210588)

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Overview

 
 
 
 
 
 

Product name

Anti-NKCC1 antibody
See all NKCC1 products (5) ...

Description

Rabbit polyclonal to NKCC1

Specificity

This antibody reacts specifically with human 132 kDa NKCC1 protein.

Tested applications

IHC-FoFr, WB, IHC-Pmore details

Cross reactivity

 

Reacts with

Mouse, Rat, Human

 

Immunogen

Synthetic peptide derived from the N terminal region of human NKCC1.

Properties

 
 
 
 
 
 
 
  • Datasheet PDF
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791) image(enlarge)

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Neuroscience >> Neurotransmitter >> Transporters >> Other Transporters
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Cardiovascular >> Hypoxia >> Associated Proteins
 

Applications

Show applications key

Our Abpromise guarantee covers the use of ab59791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    IHC-FoFr

    IHC-FoFr: Use at an assa...Read more →

    IHC-FoFr: Use at an assay dependent dilution. PubMed: 21461058

  • ShowHide1 Image

    WB

    (1 Abreview)WB: 1/100 - 1/1000. Pred...Read more →

    WB: 1/100 - 1/1000. Predicted molecular weight: 130 kDa.

  • ShowHide1 Image

    IHC-P

    IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 5 µg/ml.

Target

 
 
 
 
 
Information by UniProt
 
 

Function

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

Tissue specificity

Expressed in many tissues.

Sequence similarities

Belongs to the SLC12A transporter family.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Membrane.

Alternative names

  • Basolateral Na-K-Cl symporter antibody
  • BSC antibody
  • BSC2 antibody
see all

Database links

  • Entrez Gene: 6558 Human
  • Entrez Gene: 20496 Mouse
  • Entrez Gene: 83629 Rat
  • Omim: 600840 Human
  • SwissProt: P55011 Human
  • SwissProt: P55012 Mouse
  • Unigene: 162585 Human
  • Unigene: 712970 Human
see all

Anti-NKCC1 antibody images:

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody (ab59791)

 

 

IHC image of ab59791 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Overview

Product name

Anti-VPS29 antibody
See all VPS29 products (4) ...

Description

Goat polyclonal to VPS29

Tested applications

WBmore details

Cross reactivity

 

Reacts with

Human

Predicted to work with

Mouse, Rat, Dog

 

Immunogen

Synthetic peptide: DDVKVERIEYKKP, corresponding to C terminal amino acids 170-182 of Human VPS29.

C-DDVKVERIEYKKP

Positive control

Human muscle and kidney extracts.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

 

 

 

  • 100 µg at 0.5mg/ml

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Primary antibody notes

VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
 

  • Datasheet PDF
  • Western blot - VPS29 antibody (ab10160)Western blot - VPS29 antibody (ab10160) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa.

Target

Function

Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).

Tissue specificity

Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.

Sequence similarities

Belongs to the VPS29 family.

Cellular localization

Cytoplasm. Membrane. Endosome membrane.

Information by UniProt

Alternative names

  • DC15 antibody
  • DC7 antibody
  • DKFZp564F0223 antibody
see all

Database links

  • Entrez Gene: 51699 Human
  • Entrez Gene: 56433 Mouse
  • Entrez Gene: 288666 Rat
  • Omim: 606932 Human
  • SwissProt: Q9UBQ0 Human
  • SwissProt: Q9QZ88 Mouse
  • SwissProt: B2RZ78 Rat
  • Unigene: 600114 Human
see all

Anti-VPS29 antibody images:

  Western blot - VPS29 antibody (ab10160)

Western blot - VPS29 antibody (ab10160)

 

 

Anti-VPS29 antibody (ab10160) at 0.1 µg/ml + Human Spleen lysate (RIPA buffer, 35µg total protein per lane)

Predicted band size : 20 kDa
Observed band size : 20 kDa


Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

上海盈东生物科技有限公司

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手机:18621872635

联系人:钱先生

电话:4000210588

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(联系我时,请说明是在来宝网上看到的,谢谢!)