The Qubit® 2.0 Fluorometer is an easy-to-use, analytical instrument designed to work seamlessly with the Qubit® assays (previously known as Quant-iT™ assays) for DNA, RNA, and protein quantitation. The integrated design of the instrument and assays results in the Qubit® 2.0 Fluorometer being far more sensitive and accurate than UV absorbance; this helps avoid repetition of experiments due to inaccurate nucleic acid or protein measurements. It is ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots, and protein activity assays.
With the Qubit® 2.0 Fluorometer you get:
- A platform designed to provide highly accurate and specific measurement of DNA, RNA, or protein
- State-of-the-art optical components
- Sophisticated data analysis
- An intuitive user interface
- Small sample volume requirement (1–20 µl)
- Small bench footprint
Obtain More Accurate Results than with UV Absorbance
The Qubit® 2.0 Fluorometer is far more sensitive and accurate than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA. According to the MIQE Publication Guidelines (Ref 1), the preferred method for quantifying RNA uses fluorescent RNA-binding dyes. With the Qubit® Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit® assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.
