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Urease  U4002
Urease  U4002
  • Urease  U4002

Urease U4002

产品报价:询价

更新时间:2024/3/25 11:30:40

地:上海

牌:Sigma-Aldrich

号:20ku

厂商性质: 生产型,贸易型,服务型,

公司名称: 翌圣生物科技(上海)股份有限公司

产品关键词:

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翌圣生物科技(上海)股份有限公司 : (13916792673) (400-6111-883)

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Urease, Type IX 尿素酶

from Canavalia ensiformis (Jack Bean)

Description

Urease is involved in purine metabolism and the urea cycle. It catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide:

Urea + H2O Urease CO2 + 2 NH3

Hydroxyurea is also a substrate of the enzyme.

Unit Definition:One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms. The major protein form has a molecular weight range of 440-480 kDa and two lesser forms have molecular weight ranges of 230-260 kDa and 660-740 kDa.

This product is supplied as a lyophilized powder.

General description: Subunit molecular weight: ~90,770

Composed of six subunits with total molecular weight: ~544,620

Precautions & Disclaimer

This product is for laboratory use only. Please consult with the Material Safety Data Sheet for information regarding the hazards and safe handling practices for this product.

Preparation Instructions

The enzyme is soluble in 0.2 M sodium phosphate buffer, pH 7.0, (10 mg/ml) yielding a clear to hazy solution. The following buffers have been shown not to inhibit urease activity: MES, HEPES, and CHES.

Storage/Stability

The recommended storage temperature is 2–8 °C.

Ordering Information

Description
Quantity
Price(RMB)
促销价(RMB)
Cat. no.
产地

Urease
20KU
579
368
U4002
Sigma

100KU
1801
1269
U4002
Sigma
 



 

翌圣生物科技(上海)股份有限公司

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手机:13916792673

联系人:翌圣生物科技(上海)股份有限公司

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传真:021-34615995

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