96T 小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒
产品报价:询价
更新时间:2017/2/10 9:20:19
产地:美国
品牌:
型号:96T
厂商性质: 生产型,贸易型,服务型,
公司名称: 厦门慧嘉生物科技有限公司
陈先生 : (18906011628) (0592-6020891)
(联系我时,请说明是在来宝网上看到的,谢谢!)产品报价:询价
更新时间:2017/2/10 9:20:19
产地:美国
品牌:
型号:96T
厂商性质: 生产型,贸易型,服务型,
公司名称: 厦门慧嘉生物科技有限公司
陈先生 : (18906011628) (0592-6020891)
(联系我时,请说明是在来宝网上看到的,谢谢!)
厦门慧嘉生物长期专业销售ELISA试剂盒\Santa \ Abcam \ Cst \ jackson \ Pierce \ Sigma \ Amresco \Qiagen \ Cayman \ abnova \ millipore \ invitrogen \ merk \ ebioscience \prospec \ peprotech细胞因子特价专供。等生物试剂产品。实验为大,诚信经营,为客户提供“最高质量的产品”和“最优质的服务:18906011628 0592-6020891 QQ: 1193952234 1048735792 或登陆http://www.biohj.com(向客服人员索取原版说明书)。欢迎广大老师来询!”
小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒
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检测范围: |
Request Information |
灵敏度: |
Request Information |
反应时间: |
1-3h |
所需样本体积: |
50-100ul |
检测波长: |
450 nm |
说明书: |
(向客服人员索取原版说明书) |
原理 : |
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured. |
特异性: |
This assay has high sensitivity and excellent specificity for detection of Rat ACHE. No significant cross-reactivity or interference between Rat ACHE and analogues was observed. |
精密度: |
Intra-assay Precision (Precision within an assay): CV%<8% |
样本搜集及储存: |
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. |
检测步骤: |
Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. |
结果计算: |
Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web. |