Catalog Number

600264

Unit

0.01 mg

Price

$235.00

Format

Each vial contains 0.01 mg protein (liquid) in PBS pH7.4, 50% glycerol. Final concentration 0.1-1 mg/ml.

Description

Interleukin-12 beta (IL-12 beta) is a cytokine that can act as a growth factor for activated T and NK cells. IL-12 beta enhances the lytic activity of NK/lymphokine-activated killer cells, and stimulates the production of IFN-gamma by resting PBMC. IL-12 beta associates with IL23A to form IL-23, a heterodimeric cytokine which functions in innate and adaptive immunity. Defects in IL12 beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. Recombinant IL-12 beta comprises a 306 amino acid fragment (23-328) corresponding to the mature IL-12 beta chain and is expressed in E. coli with an amino-terminal hexahistidine tag.

Alternate Names

IL-12 beta; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2

Accession No.

P29460
 

MW

39.02 kDa

Sequence

His-Tag sequence not included: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Source

E. coli

Purity

Purity > 95% as determined by reducing and non-reducing SDS-PAGE, Bradford assay, Western blot and ELISA.

Application Notes

E, WB

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment.