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抗体信息如下：

Primary Antibodies

ATF6 Antibody

Background

Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes (2-4). ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.

Additional Names

ATF6 (NT), Activating transcription factor 6

	Description Left: Western blot analysis of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 礸/ml. Below: Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 μg/ml.
	Other Product Images

Source

ATF6 antibody was raised against a 17 amino acid peptide from near the amino terminus of human ATF6.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

ATF6 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human ATF6 (N-Terminus) Peptide (Cat. No. 3683P)

Application

ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/ml.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3683P - ATF6 Peptide

Storage

ATF6 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1217 - MDA-MB-361 Cell Lysate
2. Cat. No. 1288 - A20 Cell Lysate

Species Reactivity

H, M

Protein GI Number

56786157

Protein Accession Number

NP_031374

Short Description

(NT) An ER signaling protein of the unfolded protein response pathway

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Signal Transduction Antibodies
• Cytokine Antibodies

Related Products

• A-20 Cell Slide
  (Catalog No. 17-208).
• A-20 Lysate
  (Catalog No. 1288).
• ATF6 Antibody
  (Catalog No. 3681).
• ATF6 Peptide
  (Catalog No. 3683P).
• IRE1p Antibody
  (Catalog No. 3655).
• MDA-MB-361 Lysate
  (Catalog No. 1217).
• XBP-1 Antibody
  (Catalog No. 3685).

References

1. Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2.
2. Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
3. Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
4. Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.

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