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抗体信息如下：

Primary Antibodies

Caspase-4 Antibody

Background

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer抯 disease (5).

Additional Names

Caspase-4 (NT), ICH-2, ICE _rel II , Mih1

	Description Left: Western blot analysis of caspase-4 in Ramos cells with caspase-4 antibody at (A) 0.5 and (B) 1 μgg/ml. Below: Immunohistochemical staining of mouse spleen using caspase-4 antibody at 2 μg/ml.
	Other Product Images

Source

Caspase-4 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

Caspase-4 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human Casp-4 / ICH-2 / ICE rel II / Mih1 (N-Terminus) Peptide (Cat. No. 3451P)

Application

Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 μg/ml. Depending on cell lines or tissues used, other cleavage products may be observed.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3451P - Casp-4 Peptide

Storage

Caspase-4 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1225 - Ramos Cell Lysate
2. Cat. No. 1406 - Mouse Spleen Tissue Lysate

Species Reactivity

H, M

Protein GI Number

886050

Protein Accession Number

AAA86890

Short Description

(NT) a caspase induced by ER stress

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Alzheimer's Disease Antibodies
• Cancer Antibodies
• Neurobiology Antibodies

Related Products

• Apaf-1 Monoclonal Antibody
  (Catalog No. PM-2015).
• Apaf-1 Monoclonal Antibody
  (Catalog No. PM-2016).
• Caspase-1 Antibody
  (Catalog No. 3459).
• Caspase-4 Antibody
  (Catalog No. 3453).
• Caspase-4 Peptide
  (Catalog No. 3451P).
• Multiple Species Tissue Array - Spleen Tissue
  (Catalog No. 10-900-MSTA).
• Ramos Lysate
  (Catalog No. 1225).
• Spleen Lysate
  (Catalog No. 1406).

References

1. Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2. Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
3. Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
4. Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1 β-converting enzyme family of cysteine proteases. J. Biol. Chem. 1995; 270:15250-6.

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