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乐博现货供应美国ProSci凋亡相关--FLASH (CT) antibody
乐博现货供应美国ProSci凋亡相关--FLASH (CT) antibody
  • 乐博现货供应美国ProSci凋亡相关--FLASH (CT) antibody

乐博现货供应美国ProSci凋亡相关--FLASH (CT) antibody

产品报价:2950元

更新时间:2012/7/18 15:46:57

地:北京

牌:ProSci

号:2271

厂商性质: 生产型,贸易型,服务型,

公司名称: 北京乐博生物科技有限公司

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抗体信息如下:

Primary Antibodies

FLASH Antibody

Background

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed.

 

Additional Names

FLASH (CT), FLASH

FLASH Antibody

Description

Left: Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/ml.

Below: Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/ml.
 

Other Product Images

Source

FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human FLASH which differ from those of mouse by one amino acid.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

FLASH antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human FLASH (C-Terminus) Peptide (Cat. No. 2271P)

Application

FLASH antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 μg/ml. An approximate 220 kDa band can be detected.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 2271P - FLASH Peptide

Storage

FLASH antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

  1. Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity

H

Protein GI Number

5533376

Protein Accession Number

AF165161

Short Description

(CT) Homolog of CED4

This product belongs to the following categories:

  • Antibodies
  • Polyclonal Antibodies
  • Apoptosis Antibodies

Related Products

  • Apaf1 Antibody
    (Catalog No. 2013).
  • Caspase-8 Antibody
    (Catalog No. 3473).
  • FLASH Peptide
    (Catalog No. 2271P).
  • HeLa Cell Slide
    (Catalog No. 17-001).
  • HeLa Lysate
    (Catalog No. 1201).

References

  1. Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85
  2. Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3
  3. Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800)

 

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