N492
NB Basal Medium
Modified Chu/ Gamborg Basal Medium

 
Properties
Form:  Powder
Appearance:  White to Yellow Powder
Application:  Plant Tissue Culture
Solubility:  Water
Typical Working
4.10 g/L
Concentration:
Storage Temp:  2 ¨ 6!a  
Storage Temp of  Preparation of concentrated solutions is not recommended as insoluble
Stock Solution:  precipitates may form.
Other Notes:  Contains the macronutrients as described by Chu (1975) and the
micronutrients as described by Gamborg et al. (1968).
pH = 3.5 ¨ 4.5

 
Formula (mg/L)
Ammonium Sulfate  463                                                             Potassium Iodide    0.75
Boric Acid  3                                                                                   Potassium Nitrate   2830
Calcium Chloride, Anhydrous  125.33                                     Potassium Phosphate, Monobasic   400
Cobalt Chloride?6H2O  0.025                                                   Zinc Sulfate?7H2O    2
Cupric Sulfate?5H2O  0.025                                                      myo-Inositol   100
Na  EDTA?2H2O  37.26                                                              Nicotinic Acid (Free Acid)   1
Ferrous Sulfate?7H2O  27.8                                                      Pyridoxine?HCI   1
Magnesium Sulfate, Anhydrous  90.37                                    Thiamine?HCI    10
Manganese Sulfate?H2O  10
Molybdic Acid (Sodium Salt)?2H2O   0.25 

 
Application Notes
Plant Tissue Culture Tested