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现在位置首页>实验试剂制品>干细胞>干细胞分化> BI EndoGo™ XF Medium内皮细胞无血清培养基 05-400-1A

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BI EndoGo™ XF Medium内皮细胞无血清培养基 05-400-1A

价    格:询价

产    地:其他国家更新时间:2019/7/15 13:41:34

品    牌:BI型    号:05-400-1A

状    态:正常点击量:634

18253167905
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山东博科干细胞应用研究院有限公司

联 系 人:母经理

电     话:18253167905

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等     级: (第 5年)

性     质:生产型,贸易型,

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EndoGo? XF Medium                  货号:05-400-1A   规格:500 mL

EndoGo? XF Supplement Mix          货号:05-410-1-25   规格:2.5 mL  

BI提供一种新型的、无异源细胞动物组分的EC细胞培养体系,专为各种来源的血管内皮细胞的体外长期培养而研发设计。

内皮细胞或血管内皮是一薄层的专门上皮细胞,由一扁平细胞所组成的。它形成血管的内壁,是血管官腔内血液及其他血管壁(单层鳞状上皮)的接口。内皮细胞是沿着整个循环系统,由心脏至最小的微血管。


EndoGo? XF内皮细胞无血清培养基可用来自于血液和淋巴管的微脉管内皮细胞,还可以用于培养来在自于真皮、心、脏、肺、膀胱和脂肪组织内的内皮细胞。


EndoGo?XF与FBS-containing Medium扩增内皮细胞比较,Chemometec进行细胞技术与细胞活力测试。

HPMEC细胞在FBS-containing medium中培养传代只能传代到P4。


HDMEC (Human Dermal Microvascular Endothelial Cells) proliferation

HDMEC endothelial cells proliferation in EndoGo XF Media

HPMEC (Human Pulmonary Microvascular Endothelial Cells) proliferation

HPMEC endothelial cells proliferation in EndoGo XF Media


Cell counts and population doubling level (PDL) of HDMEC and HPMEC expanded for several passages in EndoGo? XF in comparison to commercial FBS-containing medium. Viable cells were counted using ChemoMetec Viability and Cell Count Assay.
*HPMEC did not survive P5 in the FBS-containing medium.

Macro vascular HUVEC (Human Umbilical Vein Endothelial Cells)

HUVEC endothelial cells

A

HUVEC endothelial cells morphology

B

HUVEC endothelial cells

C


Micro vascular HDMEC (Human Dermal Microvascular Endothelial Cells)

HDMEC endothelial cells

A

HDMEC endothelial cells morphology

B

HDMEC endothelial cells

C

Microvascular endothalial cells and macrovascular endothelial cells maintain classic cobblestone like morphology after expansion for several sequential passages with equal seeding (5000 cells/cm2) in EndoGo? XF +2% OTC human AB serum on hFN pre-coated dishes (A), expanded cells preserved endothelial cell features (EC markers expression) (B) and angiogenic potential to form capillary-like tubes (C).






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