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现在位置首页>实验试剂制品>蛋白纯化>磁珠及相关> Miltenyi CD34 MicroBead Kit 美天旎人CD34微珠试剂盒

商品编号:72096
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Miltenyi CD34 MicroBead Kit 美天旎人CD34微珠试剂盒

价    格:询价

产    地:德国更新时间:2019/7/15 13:41:34

品    牌:Miltenyi型    号:130-046-702

状    态:正常点击量:283

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山东博科干细胞应用研究院有限公司

联 系 人:母经理

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产品英文名称:Miltenyi CD34 MicroBeads, human 
产品中文名称:美天旎人CD34微珠试剂盒


Miltenyi CD34 MicroBead Kit 微珠试剂盒研究背景:
CD34抗原是一种单链跨膜糖蛋白表达于人类造血祖细胞,内皮祖细胞,血管内皮细胞,胚胎成纤维细胞,和一些细胞在胎儿和成人神经组织。在CD34微珠套件包直接结合微珠到CD34抗体对CD34表达细胞的磁性标记从外周血,脐带血,骨髓,血液分离收获,或分化的ES和iPS细胞。造血祖细胞,呈现在外周血中约0.05-0.2%的频率,在脐带血0.1-0.5%,并且在骨髓0.5-3%,可迅速而有效地丰富了。


美天旎人CD34微珠试剂盒产品介绍:
Components 
2 mL CD34 MicroBeads, human:
MicroBeads conjugated to monoclonal mouseanti-human CD34 antibodies (isotype: mouseIgG1).
2 mL FcR Blocking Reagent, human:
Human IgG.
or
10 mL CD34 MicroBeads, human:
MicroBeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype: mouseIgG1).
10 mL FcR Blocking Reagent, human:
Human IgG.
Capacity 
2 mL: For 2x10*9 total cells, up to 20 separations.
or
10 mL: For 10*10 total cells, up to 100 separations. 
Product format 
CD34 MicroBeads are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage 
Store protected from light at 2?8 °C. Do not freeze. The expiration date is indicated on the vial label.


Miltenyi CD34 MicroBead Kit 微珠试剂盒应用:
●正选择或细胞表达人CD34抗原。
●分离造血祖细胞。
●分离内皮祖细胞(EPCs)的。
●从分化的ES和iPS细胞培养中分离CD34+祖细胞
●在体外分化的研究。
●研究恶性血液病。

美天旎人CD34微珠试剂盒产品英文描述:
The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:
peripheral blood (PBMCs)
bone marrow1
leukapheresis product
cord blood3
differentiated ES and iPS cell lines.
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.
Isolated cells are suitable for various downstream applications, e.g., flow cytometric analysis, expansion, transduction10 or differentiation5 in culture, reprogramming3, gene expression analyses, or transplantation into humanized mice.






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